The effects of denervation on the localization of &karsi028;5-3β-hydroxysteroid dehydrogenase activity in the rat ovary during pregnancy

1977 ◽  
Vol 97 (3) ◽  
pp. 286-290 ◽  
Author(s):  
Hubert W. Burden ◽  
I. Irvin ◽  
E. Lawrence, jr
Keyword(s):  
Dehydrogenase Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Rat Ovary

scholarly journals A HISTOCHEMICAL METHOD FOR THE DEMONSTRATION OF 20α-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN RAT OVARIES

1964 ◽  
Vol 12 (9) ◽  
pp. 670-673 ◽  
Author(s):  
KÁROLY BALOGH
Keyword(s):  
Enzyme Activity ◽  
Corpus Luteum ◽  
Dehydrogenase Activity ◽  
Present Method ◽  
Hydroxysteroid Dehydrogenase ◽  
Microscopic Level ◽  
Corpora Lutea ◽  
Endocrine Glands ◽  
Progesterone Metabolism ◽  
Rat Ovary

20α-Hydroxysteroid dehydrogenase activity was localized histochemically in the corpus luteum of the rat by using Nitro-BT as an indicator. Intensive enzyme activity was obseryed in the corpus luteum cells, especially during involution. The placenta and corpora lutea of pregnancy failed to reveal enzyme activity during the last week of gravidity. Other tissues, including endocrine glands, liver and kidneys were also negative. The Present method offers a possibility to identify the sites of progesterone metabolism in the rat ovary at the microscopic level.

CHANGES IN STEROID CONCENTRATION IN THE OVARIES OF IMMATURE RATS TREATED WITH PREGNANT MARE SERUM GONADOTROPHIN AND HUMAN CHORIONIC GONADOTROPHIN

1977 ◽  
Vol 75 (1) ◽  
pp. 43-48 ◽  
Author(s):  
S. BAUMINGER ◽  
B. ECKSTEIN ◽  
H. R. LINDNER
Keyword(s):  
Dehydrogenase Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Immature Rat ◽  
Immature Rats ◽  
Rat Ovary ◽  
Pregnant Mare Serum Gonadotrophin

The concentrations of testosterone, progesterone and 20α-hydroxypregn-4-en-3-one (20α-OHP) were measured in the ovaries of immature rats in which ovulation was induced by treatment with pregnant mare serum gonadotrophin (PMSG) and, 48 h later, with human chorionic gonadotrophin (HCG). The concentration of testosterone in the tissue increased significantly 48 h after treatment with PMSG, reached a peak 4 h after the administration of HCG and declined to the basal level 4 h later. Increases in the levels of progesterone and 20α-OHP were observed 4 h after the administration of HCG. Whereas the level of 20α-OHP continued to rise during the subsequent 30 h, progesterone levels declined near the presumed time of ovulation (12 h after administration of HCG). It is concluded that 20α-hydroxysteroid dehydrogenase activity is present in the immature rat ovary before ovulation and that an increase in the production of testosterone in the ovaries of rats treated with PMSG and HCG precedes increased production of progesterone and 20α-OHP in these ovaries.

THE INFLUENCE OF HAEMOGLOBIN-S AND G6PD DEFICIENCY ON THE ACTIVITY OF THE 17β-HYDROXYSTEROID DEHYDROGENASE OF INTACT HUMAN ERYTHROCYTES

1974 ◽  
Vol 75 (4) ◽  
pp. 793-800
Author(s):  
A. O. Sogbesan ◽  
O. A. Dada ◽  
B. Kwaku Adadevoh
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Sex Steroids ◽  
G6pd Deficiency ◽  
Incubation Medium ◽  
Hydroxysteroid Dehydrogenase ◽  
G6pd Activity ◽  
Reverse Transformation ◽  
Oxidative Transformation ◽  
Haemoglobin S

ABSTRACT The 17β-hydroxysteroid dehydrogenase activity in intact erythrocytes of Nigerian patients, in particular with regard to haemoglobin genotypes and G6PD* activity was studied. The G6PD activity of the erythrocyte did not affect the oxidative transformation of testosterone to androstenedione and of oestradiol to oestrone. The reduction (reverse transformation) was inhibited in G6PD-deficient erythrocytes but this inhibition was offset by the addition of 0.025 m glucose to the incubation medium. The per cent oxidation transformation of testosterone was higher in Hb-AA than in Hb-SS erythrocytes. It is suggested that the differences may be a result of either lower enzyme activity in the Hb-SS erythrocytes or of differences in the uptake and possibly binding of sex steroids by intact Hb-SS and Hb-AA erythrocytes.

ACUTE ENZYME HISTOCHEMICAL CHANGES IN THE ZONA GLOMERULOSA OF THE RAT ADRENAL CORTEX

1968 ◽  
Vol 59 (3) ◽  
pp. 508-518
Author(s):  
J. D. Elema ◽  
M. J. Hardonk ◽  
Joh, Koudstaal ◽  
A. Arends
Keyword(s):  
Peritoneal Dialysis ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Adrenal Cortex ◽  
Isocitrate Dehydrogenase ◽  
Hydroxysteroid Dehydrogenase ◽  
Zona Glomerulosa ◽  
Acute Changes ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Histochemical Changes

ABSTRACT Acute changes in glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activity in the zona glomerulosa of the rat adrenal cortex were induced by peritoneal dialysis with 5 % glucose. Although less clear, the activity of 3β-ol-hydroxysteroid dehydrogenase also seemed to increase as well. No changes were seen in the activity of succinate dehydrogenase. Dialysis with 0.9 % NaCl had no effect on any of the enzymes investigated. The possible significance of these observations is discussed.

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