Fine structural aspects of vascular invasion of the tibial epiphyseal plate of growing rats

1968 ◽  
Vol 69 (1) ◽  
pp. 1-17 ◽  
Author(s):  
R.K. Schenk ◽  
J. Wiener ◽  
D. Spiro
Keyword(s):  
Vascular Invasion ◽  
Epiphyseal Plate ◽  
Growing Rats ◽  
Tibial Epiphyseal ◽  
Structural Aspects

scholarly journals CARTILAGE RESORPTION IN THE TIBIAL EPIPHYSEAL PLATE OF GROWING RATS

1967 ◽  
Vol 34 (1) ◽  
pp. 275-291 ◽  
Author(s):  
Robert K. Schenk ◽  
David Spiro ◽  
Joseph Wiener
Keyword(s):  
Endothelial Cells ◽  
Surface Membrane ◽  
Active Surface ◽  
Epiphyseal Plate ◽  
Electron Microscopic ◽  
Growing Rats ◽  
Ruffled Border ◽  
Tibial Epiphyseal ◽  
Cytoplasmic Processes ◽  
Epiphyseal Plates

An electron microscopic study of the tibial epiphyseal plates of growing rats reveals that the resorption of unmineralized and mineralized cartilage occurs by two different mechanisms. During resorption the unmineralized transverse cartilaginous walls between chondrocytes are invaded by capillary sprouts. At the resorption zone, numerous cytoplasmic processes derived primarily from the perivascular cells and, to a lesser extent, from the endothelial cells of the sprouts penetrate and appear to lyse the unmineralized transverse cartilaginous walls. Hydrolases released from the degenerating chondrocytes and/or capillary sprouts may also participate in this process. The second resorption mechanism involves the mineralized longitudinal cartilaginous septa. Resorption of these septa is mediated by chondroclasts whose fine structure is identical with that of osteoclasts. The active surface of the chondroclasts has a ruffled border. The surface membrane of the chondroclasts is relatively smooth on either side of the ruffled border and lies in direct apposition with the underlying mineralized cartilage. This observation suggests that the microenvironment in the zone of resorption may be maintained by the neighboring unruffled surfaces of the chondroclasts, which thus seal off and segregate the active portions of these cells.

MICROCHEMICAL ANALYSIS OF HUMAN TIBIAL GROWTH CARTILAGE IN VARIOUS FORMS OF DWARFISM

1972 ◽  
Vol 69 (4) ◽  
pp. 659-688 ◽  
Author(s):  
V. Stanescu ◽  
R. Stanescu ◽  
J. A. Szirmai
Keyword(s):  
Growth Plate ◽  
Chondroitin Sulphate ◽  
Epiphyseal Plate ◽  
Molecular Characteristics ◽  
Growth Disorders ◽  
Normal Children ◽  
Collagen Concentration ◽  
Vitamin D Resistant Rickets ◽  
Pooled Samples ◽  
Tibial Epiphyseal

ABSTRACT Microchemical determinations of glycosaminoglycans and collagen were preformed in isolated histological zones from sections of tibial epiphyseal plate biopsies obtained from children with growth disorders (pituitary dwarfism, congenital myxoedema, Turner's syndrome, Noonan's syndrome, mucopolysaccharidosis type VI, vitamin D resistant rickets and achondroplasia). Alternate sections were used for histochemical localization of glycosaminoglycans and proteins. The values were compared with those found in comparable zones of the growth plate from normal children of the same age. The chondroitin sulphate concentration (% of defatted dry wt.) in the normal epiphyseal plate increased from the resting zone towards the proliferating/hypertrophic zone; collagen exhibited a reverse trend. In some of the pathological biopsies the concentration of chondroitin sulphate was slightly decreased whereas that of collagen was slightly increased. A marked increase in the collagen concentration was found in achondroplasia. The solubility profiles of the cetylpyridinium complexes of the chondroitin sulphate fraction showed three main peaks with slight but characteristic differences in the various zones of the normal cartilage plate. Significant shifts in the proportion of these peaks were observed in several pathological biopsies, indicating possible deviations from the normal molecular characteristics of the chondroitin sulphate. Analysis of the main chondroitin sulphate fraction, obtained from pooled samples of normal tibial growth plate after fractionation on the macroscale, indicated that all three peaks contained both chondroitin-4 sulphate and chondroitin-6 sulphate and that they probably differed in their molecular weight.

Expression of Bcl-2 protein in the epiphyseal plate cartilage and trabecular bone of growing rats

1997 ◽  
Vol 108 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Ying Wang ◽  
Renée Toury ◽  
Michelle Hauchecorne ◽  
N. Balmain
Keyword(s):  
Trabecular Bone ◽  
Epiphyseal Plate ◽  
Growing Rats

Human placental lactogen inhibits growth without changing serum levels of IGF-1 in rats: an apparent specific action of the hormone

1992 ◽  
Vol 127 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Mimi H Chiang ◽  
Charles S Nicoll
Keyword(s):  
Epiphyseal Plate ◽  
Skeletal Growth ◽  
Female Rats ◽  
Placental Lactogen ◽  
Total Serum ◽  
Inhibitory Effects ◽  
Growth Inhibitory ◽  
Growth Promoting ◽  
Tibial Epiphyseal ◽  
Plate Width

Previous work in our laboratory has shown that the internal environment of rats has reduced growth-promoting activity during the second half of gestation and this condition is associated with resistance to the anabolic effects of GH. The placenta appears to be responsible for this condition but injections of estradiol plus progesterone into virgin females did not mimic it. Accordingly, it seemed worthwhile to test the effects of a placental lactogen (PL) for possible growth inhibitory effects. In the present study the effects of human (h)PL on skeletal growth in young female rats and on the growth of embryonic tissue transplants under their kidney capsules were investigated. Human (h) and bovine (b) GH, and ovine prolactin (oPRL) were also tested to determine whether the results obtained with hPL were specific. Twice daily subcutaneous injections of a high dose of hPL (10mg/day), but not of oPRL (5 mg/day) for 7 days inhibited both host tail growth and tibial epiphyseal plate width, and growth of whole 10-day embryo transplants. Injections of hGH at 1 mg/day for 8 days significantly increased host skeletal growth and growth of 12-day embryonic head transplants; at the same dose, neither bGH nor oPRL affected growth of the embryonic heads or of the host tibial epiphyseal plate width, but the bGH increased host tail growth. By contrast, the 1 mg/day dose of hPL significantly reduced the host's tibial epiphyseal plate width, tail growth, and transplant growth; lower doses of hPL (10 and 100 μg/day) were also inhibitory. Although all the hormone treatments increased total serum IGF-1 levels in the females, none of them had a significant effect when compared to saline injected control animals. Thus, the growth-inhibitory effects of hPL treatment appear to be specific to that hormone and they are not mediated by depression of serum IGF-1 levels. If these effects of hPL are mimicked by one or more of the rodent PLs, then the reduced growth-promoting activity and resistance to GH action that occurs in pregnant rats could be due to the rat PLs. These results indicate that in addition to having glucose-sparing effects in the mother, PLs could promote fetal growth by inhibiting growth of maternal tissues, which would thus spare other metabolites, such as amino acids and vitamins, for the conceptus.

Biochemical Events During Stapling of the Proximal Tibial Epiphyseal Plate in Pigs

1987 ◽  
Vol &NA; (218) ◽  
pp. 283???289 ◽  
Author(s):  
J. HERWIG ◽  
A. SCHMIDT ◽  
H. H. MATTHIAB ◽  
H. KLEEMANN ◽  
E. BUDDECKE
Keyword(s):  
Epiphyseal Plate ◽  
Tibial Epiphyseal
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