THE MICRO-ANATOMY OF THE SMALLEST BILIARY PATHWAYS IN MOUSE LIVER TISSUE

1961 ◽  
Vol 46 (1-2) ◽  
pp. 1-24 ◽  
Author(s):  
W.Th. Daems
Keyword(s):  
Liver Tissue ◽  
Mouse Liver ◽  
Micro Anatomy

Evidence that interaction of hepatocytes with the collecting (hepatic) veins triggers position-specific transcription of the glutamine synthetase and ornithine aminotransferase genes in the mouse liver

1991 ◽  
Vol 11 (12) ◽  
pp. 6050-6058
Author(s):  
F C Kuo ◽  
J E Darnell
Keyword(s):  
Blood Flow ◽  
Carbon Tetrachloride ◽  
Glutamine Synthetase ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Hepatic Veins ◽  
Surgical Removal ◽  
Ornithine Aminotransferase ◽  
Regenerating Liver ◽  
Portal Veins

We previously demonstrated that glutamine synthetase (GS) and ornithine aminotransferase (OAT) mRNAs are expressed in the mouse liver acinus preferentially in pericentral hepatocytes, that is, those immediately surrounding terminal central veins (A.L. Bennett, K.E. Paulson, R.E. Miller, and J.E. Darnell, Jr., J. Cell Biol. 105:1073-1085, 1987, and F.C. Kuo, W.L. Hwu, D. Valle, and J.E. Darnell, Jr., Proc. Natl. Acad. Sci. USA, in press). We now show that hepatocytes surrounding large collecting hepatic veins but not portal veins also express these two mRNAs. The pericentral hepatocytes are the most distal hepatocytes with respect to acinar blood flow, whereas this is not necessarily the case for hepatocytes next to the large collecting hepatic veins. This result implies that it is contact with some hepatic venous element which signals positional expression. In an effort to induce conditions that change relationships between hepatocytes and blood vessels, regenerating liver was studied. After surgical removal of two-thirds or more of the liver, there was no noticeable change in GS or OAT expression in the remaining liver tissue during regeneration. However, treatment with carbon tetrachloride (CCl4), which specifically kills pericentral hepatocytes, completely removed GS- and OAT-containing cells and promptly halted hepatic transcription of GS. Repair of CCl4 damage is associated with invasion of inflammatory and scavenging cells, which remove dead hepatocytes to allow regrowth. Only when hepatocytes resumed contact with pericentral veins were the pretreatment levels of OAT and GS mRNA and high levels of GS transcription restored.

scholarly journals CHANGES OF 8-OXO-2'-DEOXYGUANOSINE LEVEL IN MOUSE LIVER CELLS DNA IN CASE OF ACUTE TOXIC STRESS

2016 ◽  
Vol 11 (6) ◽  
pp. 68-74
Author(s):  
N. V. Marmiy ◽  
D. S. Esipov
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Pathological Change ◽  
Liver Cells ◽  
Antioxidant Systems ◽  
Toxic Stress ◽  
Laboratory Mice ◽  
Short Term ◽  
Initial Injection

The changes of the 8-oxo-2'-deoxyguanosine (8-oxo-dG)/dG ratio in the DNA of laboratory mice hepatocytes under the influence of toxic stress were studied. It was shown that the injection of carbon tetrachloride causes the growth of 8-oxo-dG level. A rapid increase in the level of 8-oxo-dG in DNA occurs during the first day of the experiment at short-term toxic stress. Subsequently, 48 hours after the initial injection, the level of 8-oxo-dG decreases to the control values. This change in the value of the biomarker can be attributed to the activation of the reparative and antioxidant systems. The subsequent injection results again in an increase of 8-oxo-dG level, and the latter only increases thereafter. This reflects the exhaustion of the reparative potential of the organism and accompanies the progress of inflammation and pathological change of the liver tissue.

scholarly journals Sizing lipid droplets from adult and geriatric mouse liver tissue via nanoparticle tracking analysis

2018 ◽  
Vol 410 (16) ◽  
pp. 3629-3638 ◽  
Author(s):  
Katherine A. Muratore ◽  
Charles P. Najt ◽  
Nicholas M. Livezey ◽  
James Marti ◽  
Douglas G. Mashek ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Mouse Liver ◽  
Lipid Droplets ◽  
Nanoparticle Tracking Analysis

Development of an Ion-Pair Reverse-Phase Liquid Chromatographic/Tandem Mass Spectrometry Method for the Determination of an 18-Mer Phosphorothioate Oligonucleotide in Mouse Liver Tissue

2005 ◽  
Vol 11 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Anthony T. Murphy ◽  
Patricia Brown-Augsburger ◽  
Rosie Z. Yu ◽  
Richard S. Geary ◽  
Stefan Thibodeaux ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Ion Pair ◽  
Tandem Mass ◽  
Phosphorothioate Oligonucleotide ◽  
Reverse Phase ◽  
Discovery Research

A quantitative method for the determination of a partially modified, 2′-ribose alkoxy 18-mer phosphorothioate oligonucleotide in liver tissue has been developed. A liquid:liquid extraction, ion-pair reverse phase chromatographic separation and tandem mass spectrometry were used to achieve a quantitation range of 125 to 10,000 ng g−1 mouse liver tissue. A total cycle time of 5 min was obtained while maintaining separation of three potential impurities. Separations were performed using a Discovery RP-Amide C16, 100 × 2 mm column packed with 5 μm particles. The separation was facilitated by the use of triethylamine (TEA) and hexafluoroisopropanol (HFIP) as ion-pair agents. The method has subsequently been used for the determination of other phosphorothioate oligonucleotides in support of discovery research.

Functional liver tissue engineering by an adult mouse liver-derived neuro-glia antigen 2-expressing stem/progenitor population

2017 ◽  
Vol 12 (1) ◽  
pp. e190-e202 ◽  
Author(s):  
Hongyu Zhang ◽  
Christopher T. Siegel ◽  
Jing Li ◽  
Jiejuan Lai ◽  
Ling Shuai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Liver Tissue Engineering ◽  
Adult Mouse Liver

Metabolomic profiling of biomarkers of liver X receptor-induced toxicity in mouse liver tissue

Metabolomics ◽  
2010 ◽  
Vol 7 (1) ◽  
pp. 54-70 ◽  
Author(s):  
Lynsey MacIntyre ◽  
Liang Zheng ◽  
Paul Scullion ◽  
Pat Keating ◽  
David G. Watson
Keyword(s):  
Liver Tissue ◽  
Mouse Liver ◽  
Liver X Receptor ◽  
Metabolomic Profiling

scholarly journals Spatial Transcriptomics to define transcriptional patterns of zonation and structural components in the mouse liver

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Franziska Hildebrandt ◽  
Alma Andersson ◽  
Sami Saarenpää ◽  
Ludvig Larsson ◽  
Noémi Van Hul ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Mouse Liver ◽  
Transcriptional Profiling ◽  
Expression Patterns ◽  
Physical Space ◽  
Clinical Pathology ◽  
Structural Components ◽  
Computational Approaches ◽  
Liver Transcriptome ◽  
Gradient Measurements

AbstractReconstruction of heterogeneity through single cell transcriptional profiling has greatly advanced our understanding of the spatial liver transcriptome in recent years. However, global transcriptional differences across lobular units remain elusive in physical space. Here, we apply Spatial Transcriptomics to perform transcriptomic analysis across sectioned liver tissue. We confirm that the heterogeneity in this complex tissue is predominantly determined by lobular zonation. By introducing novel computational approaches, we enable transcriptional gradient measurements between tissue structures, including several lobules in a variety of orientations. Further, our data suggests the presence of previously transcriptionally uncharacterized structures within liver tissue, contributing to the overall spatial heterogeneity of the organ. This study demonstrates how comprehensive spatial transcriptomic technologies can be used to delineate extensive spatial gene expression patterns in the liver, indicating its future impact for studies of liver function, development and regeneration as well as its potential in pre-clinical and clinical pathology.

scholarly journals Evidence that interaction of hepatocytes with the collecting (hepatic) veins triggers position-specific transcription of the glutamine synthetase and ornithine aminotransferase genes in the mouse liver.

1991 ◽  
Vol 11 (12) ◽  
pp. 6050-6058 ◽  
Author(s):  
F C Kuo ◽  
J E Darnell
Keyword(s):  
Blood Flow ◽  
Carbon Tetrachloride ◽  
Glutamine Synthetase ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Hepatic Veins ◽  
Surgical Removal ◽  
Ornithine Aminotransferase ◽  
Regenerating Liver ◽  
Portal Veins

We previously demonstrated that glutamine synthetase (GS) and ornithine aminotransferase (OAT) mRNAs are expressed in the mouse liver acinus preferentially in pericentral hepatocytes, that is, those immediately surrounding terminal central veins (A.L. Bennett, K.E. Paulson, R.E. Miller, and J.E. Darnell, Jr., J. Cell Biol. 105:1073-1085, 1987, and F.C. Kuo, W.L. Hwu, D. Valle, and J.E. Darnell, Jr., Proc. Natl. Acad. Sci. USA, in press). We now show that hepatocytes surrounding large collecting hepatic veins but not portal veins also express these two mRNAs. The pericentral hepatocytes are the most distal hepatocytes with respect to acinar blood flow, whereas this is not necessarily the case for hepatocytes next to the large collecting hepatic veins. This result implies that it is contact with some hepatic venous element which signals positional expression. In an effort to induce conditions that change relationships between hepatocytes and blood vessels, regenerating liver was studied. After surgical removal of two-thirds or more of the liver, there was no noticeable change in GS or OAT expression in the remaining liver tissue during regeneration. However, treatment with carbon tetrachloride (CCl4), which specifically kills pericentral hepatocytes, completely removed GS- and OAT-containing cells and promptly halted hepatic transcription of GS. Repair of CCl4 damage is associated with invasion of inflammatory and scavenging cells, which remove dead hepatocytes to allow regrowth. Only when hepatocytes resumed contact with pericentral veins were the pretreatment levels of OAT and GS mRNA and high levels of GS transcription restored.

scholarly journals A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

Parasitology ◽  
1997 ◽  
Vol 115 (1) ◽  
pp. 29-32 ◽  
Author(s):  
J. P. DALTON ◽  
S. R. DAY ◽  
A. C. DREW ◽  
P. J. BRINDLEY
Keyword(s):  
Cdna Library ◽  
Southern Blot ◽  
Blot Analysis ◽  
Liver Tissue ◽  
Southern Blot Analysis ◽  
Mouse Liver ◽  
Single Step ◽  
Step Density ◽  
Novel Method ◽  
Host Tissues

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.

Sign in / Sign up

Export Citation Format

Share Document