CHANGES IN THE NUCLEIC ACID APPARATUS OF THE RAT LIVER CELL DURING THE APPEARANCE OF BASOPHILIC RODS

1954 ◽  
Vol 22 (2-3) ◽  
pp. 272-277 ◽  
Author(s):  
Unne Stenram
Keyword(s):  
Nucleic Acid ◽  
Rat Liver ◽  
Liver Cell

A COMPARATIVE STUDY OF THE EFFECTS OF MALEIC HYDRAZIDE AND p-DIMETHYLAMINOAZOBENZENE ON RAT LIVER

1957 ◽  
Vol 35 (1) ◽  
pp. 1233-1240
Author(s):  
W. A. Mannell ◽  
H. C. Grice
Keyword(s):  
Body Weight ◽  
Nucleic Acid ◽  
Rat Liver ◽  
Liver Cell ◽  
Maleic Hydrazide ◽  
Liver Weight ◽  
Control Group ◽  
Stock Diet ◽  
Pathological Study ◽  
Number Of Cells

Rats receiving 2% maleic hydrazide (MH) in their diet and rats fed 0.06% p-dimethylaminoazobenzene (DAB), for periods up to 26 weeks, were compared with a control group on a stock diet. For the rats on DAB there was a decrease in body weight, in desoxyribose nucleic acid (DNA) per liver cell nucleus, and in the size of the average liver cell. There was an increase in liver weight, in DNA per liver, and in the number of cells per liver. These findings confirmed previous work with this compound. No significant changes were found in any of these measurements for the rats on MH. Pathological study revealed liver neoplasms in all animals fed DAB for 10 weeks or longer. No abnormal findings were reported for any rats on MH. The results indicate that maleic hydrazide, an antisprouting agent, does not produce any effects in rat liver similar to those caused by DAB, a known carcinogen.

A COMPARATIVE STUDY OF THE EFFECTS OF MALEIC HYDRAZIDE AND p-DIMETHYLAMINOAZOBENZENE ON RAT LIVER

1957 ◽  
Vol 35 (12) ◽  
pp. 1233-1240 ◽  
Author(s):  
W. A. Mannell ◽  
H. C. Grice
Keyword(s):  
Body Weight ◽  
Nucleic Acid ◽  
Rat Liver ◽  
Liver Cell ◽  
Maleic Hydrazide ◽  
Liver Weight ◽  
Control Group ◽  
Stock Diet ◽  
Pathological Study ◽  
Number Of Cells

Rats receiving 2% maleic hydrazide (MH) in their diet and rats fed 0.06% p-dimethylaminoazobenzene (DAB), for periods up to 26 weeks, were compared with a control group on a stock diet. For the rats on DAB there was a decrease in body weight, in desoxyribose nucleic acid (DNA) per liver cell nucleus, and in the size of the average liver cell. There was an increase in liver weight, in DNA per liver, and in the number of cells per liver. These findings confirmed previous work with this compound. No significant changes were found in any of these measurements for the rats on MH. Pathological study revealed liver neoplasms in all animals fed DAB for 10 weeks or longer. No abnormal findings were reported for any rats on MH. The results indicate that maleic hydrazide, an antisprouting agent, does not produce any effects in rat liver similar to those caused by DAB, a known carcinogen.

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

BIOSYNTHESE DE LA TRANSCORTINE

1969 ◽  
Vol 62 (3) ◽  
pp. 468-476 ◽  
Author(s):  
Jeanine Guidollet ◽  
Pierre Louisot
Keyword(s):  
Biological Activity ◽  
Rat Liver ◽  
Liver Cell ◽  
Binding Activity ◽  
Subcellular Level

ABSTRACT Corticosteroid-binding activity (transcortin) was investigated at the subcellular level of the rat liver cell: it was located exclusively in the cell sap, and not on the ribosomes or membranes. Oestrogens were found to increase the biological activity of this glycoprotein (corticosterone binding) in the plasma and in the cell sap. The isotopic activity of the glucidic fragments of glycoprotein (after incorporation of 14C-D-glucosamine), however, remained constant or decreased in the specific subcellular sites at which they were incorporated (membranes and cell sap). This absence of correlation between these two results is not in agreement with an induction of transcortin synthesis by oestrogens, but in favour of an activation of normally masked molecular sites.

scholarly journals Retinol and retinyl esters in parenchymal and nonparenchymal rat liver cell fractions after long-term administration of ethanol.

1985 ◽  
Vol 26 (9) ◽  
pp. 1112-1119
Author(s):  
M Rasmussen ◽  
R Blomhoff ◽  
P Helgerud ◽  
L A Solberg ◽  
T Berg ◽  
...  
Keyword(s):  
Rat Liver ◽  
Liver Cell ◽  
Term Administration ◽  
Retinyl Esters ◽  
Cell Fractions

Type of dietary fat alters rat liver cell responsiveness to vasopressin

1991 ◽  
Vol 2 (2) ◽  
pp. 87-90 ◽  
Author(s):  
William L. Blake ◽  
Steven D. Clarke
Keyword(s):  
Rat Liver ◽  
Liver Cell ◽  
Dietary Fat

Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver

1978 ◽  
Vol 234 (3) ◽  
pp. C122-C130 ◽  
Author(s):  
D. M. Bissell ◽  
G. A. Levine ◽  
M. J. Bissell
Keyword(s):  
Glucose Metabolism ◽  
Primary Culture ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Time Course ◽  
Primary Cultures ◽  
Functional Change ◽  
Permanent Cell ◽  
Rat Hepatoma

The metabolic fate of [U-14C]glucose has been examined in detail in adult rat hepatocytes in primary monolayer culture, as well as in two permanent cell lines--Buffalo rat liver (BRL) and transplantable rat hepatoma (HTC) cells-derived from normal rat liver and from rat hepatoma, respectively. Under defined conditions of incubation, at a glucose concentration of 5.5 mM, the three types of cultured liver cells exhibited pronounced differences in glucose metabolism. Primary cultures, like the intact liver, differed from the cell lines in consuming relatively small amounts of glucose and converting approximately 50% of the total metabolized glucose to lactate. By contrast, the permantent cell lines consumed glucose at a 40-fold greater rate than did primary cultures, converting 80--90% of the carbohydrate to lactate. Oxidative metabolism of glucose carbon also differed among the three types of liver culture. Of the total [U-14C]glucose consumed, primary cultures converted approximately 30% to labeled CO2 per hour, whereas the liver cell lines converted 5--10%. Finally, glucose metabolism in primary culture exhibited adaptation as hepatocytes aged in culture, shifting progressively toward the pattern exhibited by the permanent cell lines. This change occurred over a time course similar to that for other kinds of functional change in hepatocytes in primary culture and thus may be relevant to the general problem of phenotypic alteration in liver cell culture.

Sign in / Sign up

Export Citation Format

Share Document