Determination of Ro 15-1788, a Benzodiazepine Antagonist, in Human Plasma by Gas-Liquid Chromatography with Nitrogen-Phosphorus Detection

Pharmacology ◽  
1983 ◽  
Vol 26 (5) ◽  
pp. 285-289 ◽  
Author(s):  
Darrell R. Abernethy ◽  
Rainer M. Arendt ◽  
Peter M. Lauven ◽  
David J. Greenblatt
Keyword(s):  
Liquid Chromatography ◽  
Human Plasma ◽  
Gas Liquid Chromatography ◽  
Benzodiazepine Antagonist ◽  
Nitrogen Phosphorus

Extraction and Cleanup of Fish, Sediment, and Water for Determination of Triaryl Phosphates by Gas-Liquid Chromatography

1981 ◽  
Vol 64 (1) ◽  
pp. 79-84
Author(s):  
Derek C G Muir ◽  
Norbert P Grift ◽  
John Solomon
Keyword(s):  
Liquid Chromatography ◽  
Soxhlet Extraction ◽  
Gel Permeation Chromatography ◽  
Gas Liquid Chromatography ◽  
Fish Samples ◽  
Alumina Treatment ◽  
Diphenyl Phosphate ◽  
Nitrogen Phosphorus ◽  
Acetone Hexane

Abstract Several techniques were evaluated for extracting triphenyl phosphate (TPP), 14C-labeled TPP, cresyl diphenyl phosphate, and tricresyl phosphate isomers (o-TCP, m-TCP, and p-TCP) from fish and sediment samples. Extracts of fish samples were cleaned up by gel permeation chromatography/alumina column chromatography; sediment extracts received alumina treatment only. Compounds were determined by gas-liquid chromatography (GLC) with nitrogenphosphorus detection. Methanol/Polytron and hexane/ball mill extraction of fish samples fortified at 0.01, 0.1, and 1.0 μg/g levels gave overall recoveries of the 5 compounds of 89 and 97%, respectively. Methanol recovered more radioactivity (97%) from fish exposed to 14C-TPP in aquaria for 24 h than did hexane from fish exposed for 16 h (79%). Refluxing fortified sediment (0.05 and 0.5 μg/g) with methanol-water (9+1) gave significantly higher recoveries (88%) of the 5 triaryl phosphates than did dichloromethane-methanol (1+1) reflux or acetone-hexane (1+1) Soxhlet extraction. Recoveries of TPP and o-, m- and p-TCP from fortified river water (0.5, 5.0, and 50 μg/L) by shaking with dichloromethane ranged from 91 to 118%. Some problems were encountered with interfering GLC peaks at low (μg/g) levels in fish and sediment extracts despite the use of nitrogen-phosphorus specific detectors.

Analysis of Potato Glycoalkaloids by Gas-Liquid Chromatography of Alkaloid Components

1980 ◽  
Vol 63 (6) ◽  
pp. 1226-1230 ◽  
Author(s):  
Russell R King
Keyword(s):  
Liquid Chromatography ◽  
Lower Limit ◽  
Direct Determination ◽  
Gas Liquid Chromatography ◽  
Potato Tubers ◽  
Potato Glycoalkaloids ◽  
Nitrogen Phosphorus Detector ◽  
Liquid Chromatographic ◽  
Nitrogen Phosphorus

Abstract A gas-liquid chromatographic (GLC) method has been developed for the analysis of potato glycoalkaloids by direct determination of the alkaloid components isolated by hydrolysis with IN ethanolic HCl. Recoveries of solanidine from potato tubers and foliage fortified with solanine over a range approximately 0.5 to 5 times the glycoalkaloid originally present averaged greater than 95%, and the lower limit of sensitivity was 0.25 mg/100 g using a nitrogen-phosphorus detector. A means of distinguishing solanidine and demissidine by formation of their respective 3β-trifluoroacetates with trifluoroacetic anhydride (TFAA) is demonstrated.

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