Report of the Fourth International Workshop on Human Chromosome 16 Mapping 1995

1996 ◽  
Vol 72 (4) ◽  
pp. 271-293 ◽  
Author(s):  
Martijn H. Breuning ◽  
Norman A. Doggett ◽  
David F. Callen
Keyword(s):  
Human Chromosome ◽  
International Workshop ◽  
Chromosome 16

Human chromosome 16 physical map: Mapping of somatic cell hybrids using multiplex PCR deletion analysis of sequence tagged sites

Genomics ◽  
1991 ◽  
Vol 10 (4) ◽  
pp. 1047-1052 ◽  
Author(s):  
Robert I. Richards ◽  
Katherine Holman ◽  
Sharon Lane ◽  
Grant R. Sutherland ◽  
David F. Callen
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Multiplex Pcr ◽  
Physical Map ◽  
Deletion Analysis ◽  
Somatic Cell Hybrids ◽  
Chromosome 16 ◽  
Cell Hybrids ◽  
Sequence Tagged Sites

Report of the Fourth International Workshop on Human Chromosome 18 Mapping 1996

1996 ◽  
Vol 75 (2-3) ◽  
pp. 111-131 ◽  
Author(s):  
Gary A. Silverman ◽  
Joan Overhauser ◽  
Steve Gerken ◽  
Ad Geurts van Kessel
Keyword(s):  
Human Chromosome ◽  
International Workshop ◽  
Chromosome 18

scholarly journals Regulatory factors specific for adult and embryonic globin genes may govern their expression in erythroleukemia cells

Blood ◽  
1985 ◽  
Vol 65 (3) ◽  
pp. 705-712 ◽  
Author(s):  
NP Anagnou ◽  
TY Yuan ◽  
E Lim ◽  
J Helder ◽  
S Wieder ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Chromosome ◽  
Globin Gene ◽  
Globin Genes ◽  
Chromosome 16 ◽  
Regulatory Factors ◽  
Erythroleukemia Cells ◽  
Erythroleukemia Cell ◽  
Globin Gene Expression ◽  
Mouse Erythroleukemia

Abstract In order to test if trans-acting regulatory factors specific for globin genes of the adult and embryonic stages of development exist in erythroid cells, transcriptionally active embryonic and adult globin genes on the same chromosome were transferred by cell fusion from the human leukemia cell K562 into phenotypically adult mouse erythroleukemia cells. Restriction-fragment-length polymorphisms of the K562 zeta (embryonic) globin genes were used to establish that all three copies of human chromosome 16 present in the K562 cell showed the same pattern of human globin gene expression after transfer to the mouse erythroleukemia cell. Adult (alpha) but not embryonic (zeta) human globin mRNA was detected in all nine of the independently derived mouse erythroleukemia hybrid cells, each of which contained human chromosome 16. Restriction endonuclease studies of the K562 alpha- and zeta-globin genes after transfer into the mouse erythroleukemia cell showed no evidence of rearrangements or deletions that could explain this loss of zeta-globin gene expression. These data suggest that regulation of globin gene expression in these erythroleukemia cells involves trans-acting regulatory factors specific for the adult and embryonic stages of development.

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