The rat Prm3gene is an intronless member of the protamine gene cluster and is expressed in haploid male germ cells

1995 ◽  
Vol 71 (4) ◽  
pp. 352-355 ◽  
Author(s):  
G. Schlüter ◽  
W. Engel
Keyword(s):  
Gene Cluster ◽  
Germ Cells ◽  
Male Germ Cells ◽  
Haploid Male ◽  
I Gene

scholarly journals The RHOX homeobox gene cluster is selectively expressed in human oocytes and male germ cells

2013 ◽  
Vol 28 (6) ◽  
pp. 1635-1646 ◽  
Author(s):  
H. W. Song ◽  
R. A. Anderson ◽  
R. A. Bayne ◽  
J. Gromoll ◽  
S. Shimasaki ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Germ Cells ◽  
Homeobox Gene ◽  
Male Germ Cells ◽  
Human Oocytes

scholarly journals Structure and expression of the mouse gene encoding the endozepine-like peptide from haploid male germ cells

2000 ◽  
Vol 267 (17) ◽  
pp. 5438-5449 ◽  
Author(s):  
Mette Valentin ◽  
Marga Balvers ◽  
Wolfgang Pusch ◽  
Gerhard F. Weinbauer ◽  
Jens Knudsen ◽  
...  
Keyword(s):  
Germ Cells ◽  
Mouse Gene ◽  
Gene Encoding ◽  
Male Germ Cells ◽  
Haploid Male

scholarly journals Functional cooperation between CREM and GCNF directs gene expression in haploid male germ cells

2010 ◽  
Vol 38 (7) ◽  
pp. 2268-2278 ◽  
Author(s):  
Mirjana Rajković ◽  
K. Alexander H. Iwen ◽  
Peter J. Hofmann ◽  
Angelika Harneit ◽  
Joachim M. Weitzel
Keyword(s):  
Gene Expression ◽  
Germ Cells ◽  
Male Germ Cells ◽  
Haploid Male

scholarly journals miRNA-dependent poly(A) length control in uncoupling transcription and translation of haploid male germ cells

2021 ◽  
Author(s):  
Chong Tang ◽  
Mei Guo ◽  
Zhuoxing Shi ◽  
Zhuqing Wang ◽  
Chunhai Luo ◽  
...  
Keyword(s):  
Germ Cells ◽  
Regulatory Mechanisms ◽  
Translational Repression ◽  
Dynamic Changes ◽  
Male Germ Cells ◽  
Haploid Male ◽  
Transcriptional Regulatory ◽  
Delayed Translation ◽  
Transcriptional Regulatory Mechanisms

AbstractAs one of the post-transcriptional regulatory mechanisms, transcription and translation’s uncoupling plays an essential role in development and adulthood physiology. However, it remains elusive how thousands of mRNAs get translationally silenced while stability is maintained for up to hours or even days before translation. In addition to oocytes and neurons, developing spermatids have significant uncoupling of transcription and translation for delayed translation. Therefore, spermiogenesis represents an excellent in vivo model for investigating the mechanism underlying uncoupled transcription and translation. Through full-length poly(A) deep sequencing, we discovered dynamic changes in poly(A) length through deadenylation and re-polyadenylation. Deadenylation appeared to be mediated by microRNAs (miRNAs), and transcripts with shorter poly(A) tails tend to be sequestered into ribonucleoproteins (RNPs) for translational repression and stabilization. In contrast, re-polyadenylation allows for translocation of the translationally repressed transcripts from RNPs to polysomes for translation. Overall, our data suggest that miRNA-dependent poly(A) length control represents a novel mechanism underlying uncoupled translation and transcription in haploid male germ cells.

The mouse transcription factor Stat4 is expressed in haploid male germ cells and is present in the perinuclear theca of spermatozoa

1997 ◽  
Vol 110 (14) ◽  
pp. 1543-1553 ◽  
Author(s):  
G. Herrada ◽  
D.J. Wolgemuth
Keyword(s):  
Transcription Factor ◽  
Germ Cells ◽  
Nuclear Translocation ◽  
Biological Effects ◽  
Immunoblot Analysis ◽  
Male Germ Cells ◽  
Haploid Male ◽  
Zygotic Transcription ◽  
The Hours ◽  
Perinuclear Theca

STAT (signal transducer and activator of transcription) proteins have been shown to be essential transcription factors which mediate biological effects of cytokines. Although most of the STATs have been shown to be widely expressed, Stat4 mRNA has been detected in only a few tissues, including the testis. In the present study, immunoblot analysis confirmed that the presence of Stat4 protein was similarly restricted, with the highest level observed in testis. In situ hybridization, immunoblot, and immunohistochemistry analyses revealed that in the testis, Stat4 was abundantly and exclusively expressed in male germ cells which have completed meiosis, at the round and elongating spermatid stages. Cytolocalization at various times of spermatid differentiation showed that the level of Stat4 protein increased in parallel in both cytoplasm and nuclei. No specific nuclear translocation that would have been an indicator of Stat4 activation was observed at any stage of spermatogenic differentiation. Interestingly, the Stat4 transcription factor was localized to the condensing perinuclear theca of spermatids, a localization that was confirmed by selective biochemical extraction of thecal proteins. Since the theca is known to depolymerize in the cytoplasm of the oocyte during the hours following fertilization, we hypothesized that sperm Stat4 would represent an original paternal contribution to the fertilized egg which may be involved in the onset of zygotic transcription.

scholarly journals Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 745 ◽  
Author(s):  
Fahar Ibtisham ◽  
Ali Honaramooz
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Recent Progress ◽  
Male Germ Cells ◽  
Culture Systems ◽  
In Vitro Spermatogenesis ◽  
Haploid Male ◽  
Restoration Of Fertility

Spermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genes onto the next generation. SSCs also have the potential to provide important knowledge about stem cells in general and to offer critical in vitro and in vivo applications in assisted reproductive technologies. After century-long research, proof-of-principle culture systems have been introduced to support the in vitro differentiation of SSCs from rodent models into haploid male germ cells. Despite recent progress in organotypic testicular tissue culture and two-dimensional or three-dimensional cell culture systems, to achieve complete in vitro spermatogenesis (IVS) using non-rodent species remains challenging. Successful in vitro production of human haploid male germ cells will foster hopes of preserving the fertility potential of prepubertal cancer patients who frequently face infertility due to the gonadotoxic side-effects of cancer treatment. Moreover, the development of optimal systems for IVS would allow designing experiments that are otherwise difficult or impossible to be performed directly in vivo, such as genetic manipulation of germ cells or correction of genetic disorders. This review outlines the recent progress in the use of SSCs for IVS and potential in vivo applications for the restoration of fertility.

PSCDGs of mouse multipotent adult germline stem cells can enter and progress through meiosis to form haploid male germ cells in vitro

2010 ◽  
Vol 80 (4-5) ◽  
pp. 184-194 ◽  
Author(s):  
Jessica Nolte ◽  
Hans W. Michelmann ◽  
Marieke Wolf ◽  
Gerald Wulf ◽  
Karim Nayernia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Germline Stem Cells ◽  
Male Germ Cells ◽  
Haploid Male

Retromer vesicles interact with RNA granules in haploid male germ cells

2015 ◽  
Vol 401 ◽  
pp. 73-83 ◽  
Author(s):  
Matteo Da Ros ◽  
Noora Hirvonen ◽  
Opeyemi Olotu ◽  
Jorma Toppari ◽  
Noora Kotaja
Keyword(s):  
Germ Cells ◽  
Male Germ Cells ◽  
Rna Granules ◽  
Haploid Male
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