scholarly journals Confirmed assignment of a novel human tyrosine kinase gene (JAK1A) to 1p32.3→p31.3 by nonisotopic in situ hybridization

1995 ◽  
Vol 69 (3-4) ◽  
pp. 232-234 ◽  
Author(s):  
W.S. Modi ◽  
W.L. Farrar ◽  
O.M.Z. Howard
Keyword(s):  
In Situ Hybridization ◽  
Tyrosine Kinase ◽  
Kinase Gene ◽  
Tyrosine Kinase Gene

Common Fluorescence In Situ Hybridization Applications in Cytology

2016 ◽  
Vol 140 (12) ◽  
pp. 1323-1330 ◽  
Author(s):  
Spasenija Savic ◽  
Lukas Bubendorf
Keyword(s):  
In Situ Hybridization ◽  
Tyrosine Kinase ◽  
Fluorescence In Situ Hybridization ◽  
Kinase Inhibitors ◽  
Molecular Testing ◽  
Diagnostic Dilemma ◽  
Kinase Gene ◽  
Anaplastic Lymphoma ◽  
Genomic Aberrations

Context.— Fluorescence in situ hybridization (FISH) is a well-established method for detection of genomic aberrations in diagnostic, prognostic, and predictive marker testing. Objective.— To review common applications of FISH in cytology. Data Sources.— The published literature was reviewed. Conclusions.— Cytology is particularly well suited for all kinds of FISH applications, which is highlighted in respiratory tract cytology with an increasing demand for predictive FISH testing in lung cancer. Fluorescence in situ hybridization is the gold standard for detection of predictive anaplastic lymphoma kinase gene (ALK) rearrangements, and the same evaluation criteria as in histology apply to cytology. Several other gene rearrangements, including ROS proto-oncogene 1 receptor tyrosine kinase (ROS1), are becoming clinically important and share the same underlining cytogenetic mechanisms with ALK. MET amplification is one of the most common mechanisms of acquired resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors and can be targeted by crizotinib. As genomic aberrations are a hallmark of malignant cells, FISH is a valuable objective ancillary diagnostic tool. In urinary tract cytology, atypical urothelial cells equivocal for malignancy are a common diagnostic dilemma and multitarget FISH can help clarify such cells. Diagnosis of malignant mesothelioma remains one of the most challenging fields in effusion cytology, and ancillary FISH is useful in establishing the diagnosis. Fluorescence in situ hybridization is a morphology-based technique, and the prerequisite for reliable FISH results is a targeted evaluation of the cells in question (eg, cancer or atypical cells). Cytopathologists and cytotechnicians should therefore be involved in molecular testing in order to select the best material and to provide their morphologic expertise.

Diverse expression of dsrc29A, a gene related to src, during the life cycle of Drosophila melanogaster

Development ◽  
1990 ◽  
Vol 110 (4) ◽  
pp. 1169-1183
Author(s):  
A.L. Katzen ◽  
T. Kornberg ◽  
J.M. Bishop
Keyword(s):  
Life Cycle ◽  
Tyrosine Kinase ◽  
Protein Tyrosine Kinase ◽  
Kinase Domain ◽  
Tyrosine Kinase Domain ◽  
Phagocytic Cells ◽  
Kinase Gene ◽  
Protein Tyrosine ◽  
Tyrosine Kinase Gene

We used in situ hybridization to study the RNA expression of the dsrc29A gene during Drosophila development. This gene encodes two proteins differing at their amino termini. Both gene products contain a protein-tyrosine kinase domain and resemble the protein encoded by vertebrate src. We examined most stages of development in the Drosophila life cycle: embryos, third instar larvae, pupae and adults. Our results revealed that dsrc29A expression is specialized throughout development, being prominent at various times in neural tissue, phagocytic cells, dorsal vessel, ovaries, gut, developing salivary glands, imaginal discs and disc derivatives. These findings confirm and extend previous results for the distribution of dsrc29A protein, indicating that the regulation of this gene is primarily at the level of transcription. In some tissues expression is transient, whereas in others, it is continuous, and expression occurs in proliferative, differentiating and differentiated tissue. These patterns of expression demonstrate how a single protein-tyrosine kinase might play diverse roles at different times during development. Comparison of the expression of dsrc29A and other members of the protein-tyrosine kinase gene superfamily reveals that the genes are expressed in distinctive but sometimes overlapping patterns.

scholarly journals Distinct Mutations in the Receptor Tyrosine Kinase Gene ROR2 Cause Brachydactyly Type B

2000 ◽  
Vol 67 (4) ◽  
pp. 822-831 ◽  
Author(s):  
Georg C. Schwabe ◽  
Sigrid Tinschert ◽  
Christian Buschow ◽  
Peter Meinecke ◽  
Gerhard Wolff ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Receptor Tyrosine Kinase ◽  
Type B ◽  
Kinase Gene ◽  
Tyrosine Kinase Gene ◽  
Receptor Tyrosine Kinase Gene

FLT4 Receptor Tyrosine Kinase Gene Mapping to Chromosome Band 5q35 in Relation to the t(2;5), t(5;6), and t(3;5) Translocations

1993 ◽  
Vol 7 (3) ◽  
pp. 144-151 ◽  
Author(s):  
Elina Armstrong ◽  
Kumar Kastury ◽  
Olga Aprelikova ◽  
Florencia Bullrich ◽  
Christian Nezelof ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Gene Mapping ◽  
Receptor Tyrosine Kinase ◽  
Chromosome Band ◽  
Kinase Gene ◽  
Tyrosine Kinase Gene ◽  
Receptor Tyrosine Kinase Gene

The testis-specific transcript (ferT) of the tyrosine kinase FER is expressed during spermatogenesis in a stage-specific manner

1990 ◽  
Vol 10 (9) ◽  
pp. 5021-5025
Author(s):  
E Keshet ◽  
A Itin ◽  
K Fischman ◽  
U Nir
Keyword(s):  
In Situ Hybridization ◽  
Tyrosine Kinase ◽  
Tyrosine Kinases ◽  
Meiotic Prophase ◽  
Hybridization Analysis ◽  
Pachytene Stage ◽  
Specific Transcript ◽  
Specific Manner

ferT is a testis-specific transcript of FER encoding a truncated version of the potential tyrosine kinase. Using in situ hybridization analysis, we found that ferT was transiently expressed during spermatogenesis and that expression was restricted to spermatocytes at the pachytene stage of meiotic prophase. This pattern of expression is unprecedented by other tyrosine kinases and suggests a role for ferT in a particular stage of spermatogenesis.

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