Expression of heterochromatin by restriction endonuclease treatment and distamycin A/DAPI staining of Indian muntjac (Muntiacus muntjak) chromosomes

1986 ◽  
Vol 41 (2) ◽  
pp. 96-100 ◽  
Author(s):  
A. Babu ◽  
R.S. Verma
Keyword(s):  
Restriction Endonuclease ◽  
Dapi Staining ◽  
Distamycin A ◽  
Indian Muntjac ◽  
Muntiacus Muntjak

Heterochromatin organization in the nucleus of Indian muntjac (Muntiacus muntjak)

1986 ◽  
Vol 28 (4) ◽  
pp. 628-630 ◽  
Author(s):  
Ram S. Verma ◽  
Jessey P. Jacob ◽  
Arvind Babu
Keyword(s):  
Restriction Endonuclease ◽  
Y Chromosome ◽  
X Chromosome ◽  
General Pattern ◽  
Banding Technique ◽  
Chromosome Y ◽  
Heterochromatic Segment ◽  
Indian Muntjac ◽  
Muntiacus Muntjak

The heterochromatin in Indian muntjac (Muntiacus muntjak) is located at the periphery of primary constrictions of all the chromosomes. The X chromosome contains significantly larger amounts of heterochromatin than the rest of the complement by C-banding technique. However, the small portion of C-band region was found to be resistant by restriction endonuclease HaeIII (5′… GG ↓ CC … 3′) and was clearly visible on the nucleus. Therefore, the position of this large heterochromatic segment is examined at somatic metaphases. The distribution of the heterochromatin of the X chromosome observed in Indian muntjac is contrary to the general pattern observed in other species, i.e., the chromosomes consisting greater amount of heterochromatin are located more peripherally than those with lesser amount. However, the smaller Y chromosome (Y1) is frequently found at the periphery. The present findings suggest that the role of heterochromatin organization in the nucleus vary between different heterochromatic segments of the same species and vary from species to species.Key words: heterochromatin, chromosome, nucleus, metaphase, Muntiacus muntjak.

Heterochromatin composition and nucleolus organizer activity in four canid species

1986 ◽  
Vol 28 (5) ◽  
pp. 744-753 ◽  
Author(s):  
B. Mayr ◽  
G. Geber ◽  
H. Auer ◽  
M. Kalat ◽  
W. Schleger
Keyword(s):  
Organizer Region ◽  
Nucleolus Organizer Region ◽  
Nucleolus Organizer ◽  
Domestic Dog ◽  
Banding Technique ◽  
Dapi Staining ◽  
Distamycin A ◽  
Close Relationship ◽  
Heterochromatin Polymorphism ◽  
Canid Species

Sequential staining with a counterstain-contrasted fluorescent banding technique (chromomycin A3 – distamycin A – DAPI) revealed the occurrence of distamycin A – 4,6-diamidino-2-phenylindole (DA–DAPI) staining heterochromatin in the centromeric regions of chromosomes 33, 36, 37, and 38 in the wolf (Canis lupus pallipes) and of chromosomes 13, 16, and 23 in the blue fox (Alopex lagopus). The red fox (Vulpes vulpes) lacked such regions. Staining with DAPI – actinomycin D produced a QFH-type banding pattern with clearcut differences in the staining behaviour of DA–DAPI positive regions between these three canid species. Staining with the fluorochrome D 287/170 did not preferentially highlight any of the DA–DAPI positive regions in any of them. Counterstain-enhanced chromomycin A3 R-banding and studies of nucleolus organizer region location and activity confirmed a close relationship between the karyotype of the wolf and the domestic dog. Few heterochromatic marker bands were encountered in these two species, but heterochromatin polymorphism was evident in the blue fox.Key words: Canidae, heterochromatin, nucleolus organizers.

Comparative genomic analysis links karyotypic evolution with genomic evolution in the Indian Muntjac (Muntiacus muntjak vaginalis)

Chromosoma ◽  
2006 ◽  
Vol 115 (6) ◽  
pp. 427-436 ◽  
Author(s):  
Qi Zhou ◽  
Ling Huang ◽  
Jianguo Zhang ◽  
Xiangyi Zhao ◽  
Qingpeng Zhang ◽  
...  
Keyword(s):  
Genomic Analysis ◽  
Comparative Genomic Analysis ◽  
Comparative Genomic ◽  
Karyotypic Evolution ◽  
Genomic Evolution ◽  
Indian Muntjac ◽  
Muntiacus Muntjak

Comparative Chromosome Painting in Mammals: Human and the Indian Muntjac (Muntiacus muntjak vaginalis)

Genomics ◽  
1997 ◽  
Vol 39 (3) ◽  
pp. 396-401 ◽  
Author(s):  
Fengtang Yang ◽  
Stefan Müller ◽  
Reinhard Just ◽  
Malcolm A. Ferguson-Smith ◽  
Johannes Wienberg
Keyword(s):  
Chromosome Painting ◽  
Comparative Chromosome Painting ◽  
Indian Muntjac ◽  
Muntiacus Muntjak

CONSTITUTIVE HETEROCHROMATIN OF INDIAN MUNTJAC CHROMOSOMES REVEALED BY DNASE TREATMENT AND A C-BANDING TECHNIQUE

1974 ◽  
Vol 16 (2) ◽  
pp. 273-280 ◽  
Author(s):  
H. Kato ◽  
K. Tsuchiya ◽  
T. H. Yosida
Keyword(s):  
Secondary Constriction ◽  
Constitutive Heterochromatin ◽  
Banding Technique ◽  
Size Difference ◽  
Indian Muntjac ◽  
C Banding ◽  
Dnase Treatment ◽  
Muntiacus Muntjak ◽  
Secondary Constrictions ◽  
Constriction Region

A karyotype of a female Indian muntjac, Muntiacus muntjak vaginalis, was described. The karyotype was unique in that No. 1 and No. 3 homologous pairs were heteromorphic with respect to the size of their secondary constrictions. In these pairs, one of the homologs always had a longer secondary constriction than that on the corresponding homolog. Heterochromatin in the secondary constriction region was visualized with difficulty by a C-banding technique, but was demonstrated clearly by a DNase treatment followed by Giemsa staining, which also revealed the size difference of the secondary constriction. Centromeric constitutive heterochromatin of No. 1 chromosome was also found to differ in size between the homologs. On the basis of the heteromorphic character of No. 3 chromosome, or an X-autosome complex, it was possible to confirm autoradiographically that X-inactivation had occurred at random.

Heterogeneity of human chromosome 9 constitutive heterochromatin as revealed by sequential distamycin A/DAPI staining and C-banding

1981 ◽  
Vol 57 (1) ◽  
pp. 28-30 ◽  
Author(s):  
C. H. C. M. Buys ◽  
W. L. Gouw ◽  
J. A. M. Blenkers ◽  
C. H. van Dalen
Keyword(s):  
Human Chromosome ◽  
Constitutive Heterochromatin ◽  
Chromosome 9 ◽  
Dapi Staining ◽  
Distamycin A ◽  
C Banding
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