Localization of mouse parathyroid hormone-like peptide gene (Pthlh) to distal chromosome 6 using interspecific backcross mice and in situ hybridization

1992 ◽  
Vol 60 (3-4) ◽  
pp. 252-254
Author(s):  
M.F. Seldin ◽  
M.G. Mattei ◽  
G.N. Hendy
Keyword(s):  
In Situ Hybridization ◽  
Parathyroid Hormone ◽  
Interspecific Backcross ◽  
Chromosome 6 ◽  
Distal Chromosome ◽  
Peptide Gene

Recovery of a telomere-truncated chromosome via a compensating translocation in maize

Genome ◽  
2011 ◽  
Vol 54 (3) ◽  
pp. 184-195 ◽  
Author(s):  
Robert T. Gaeta ◽  
Tatiana V. Danilova ◽  
Changzeng Zhao ◽  
Rick E. Masonbrink ◽  
Morgan E. McCaw ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Transgene Expression ◽  
De Novo ◽  
Extra Chromosome ◽  
Single Gene ◽  
B Chromosomes ◽  
Chromosome 1 ◽  
Chromosome 6

Maize-engineered minichromosomes are easily recovered from telomere-truncated B chromosomes but are rarely recovered from A chromosomes. B chromosomes lack known genes, and their truncation products are tolerated and transmitted during meiosis. In contrast, deficiency gametes resulting from truncated A chromosomes prevent their transmission. We report here a de novo compensating translocation that permitted recovery of a large truncation of chromosome 1 in maize. The truncation (trunc-1) and translocation with chromosome 6 (super-6) occurred during telomere-mediated truncation experiments and were characterized using single-gene fluorescent in situ hybridization (FISH) probes. The truncation contained a transgene signal near the end of the broken chromosome and transmitted together with the compensating translocation as a heterozygote to approximately 41%–55% of progeny. Transmission as an addition chromosome occurred in ~15% of progeny. Neither chromosome transmitted through pollen. Transgene expression (Bar) cosegregated with trunc-1 transcriptionally and phenotypically. Meiosis in T1 plants revealed eight bivalents and one tetravalent chain composed of chromosome 1, trunc-1, chromosome 6, and super-6 in diplotene and diakinesis. Our data suggest that de novo compensating translocations allow recovery of truncated A chromosomes by compensating deficiency in female gametes and by affecting chromosome pairing and segregation. The truncated chromosome can be maintained as an extra chromosome or together with the super-6 as a heterozygote.

Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping

Chromosoma ◽  
2010 ◽  
Vol 119 (4) ◽  
pp. 435-442 ◽  
Author(s):  
Qunfeng Lou ◽  
Marina Iovene ◽  
David M. Spooner ◽  
C. Robin Buell ◽  
Jiming Jiang
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Solanum Species ◽  
Chromosome 6 ◽  
Hybridization Mapping

Localization of parathyroid hormone-related protein in osteoclasts by in situ hybridization and immunohistochemistry

Bone ◽  
1998 ◽  
Vol 22 (3) ◽  
pp. 189-194 ◽  
Author(s):  
V. Kartsogiannis ◽  
N. Udagawa ◽  
K.W. Ng ◽  
T.J. Martin ◽  
J.M. Moseley ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Parathyroid Hormone ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

Localization of parathyroid hormone-related protein mrna expression in breast cancer and metastatic lesions by in situ hybridization

2009 ◽  
Vol 7 (8) ◽  
pp. 971-979 ◽  
Author(s):  
Socorro J. Vargas ◽  
Matthew T. Gillespie ◽  
Gerard J. Powell ◽  
Justine Southby ◽  
Janine A. Danks ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Parathyroid Hormone ◽  
Mrna Expression ◽  
Related Protein ◽  
Metastatic Lesions ◽  
Parathyroid Hormone Related Protein
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