Sensitivity of human-mouse somatic cell hybrids to human interferon

1978 ◽  
Vol 22 (1-6) ◽  
pp. 270-274
Author(s):  
D.L. Slate ◽  
F.H. Ruddle
Keyword(s):  
Somatic Cell ◽  
Human Interferon ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Mouse Somatic Cell

Genetic Analysis with Man–Mouse Somatic Cell Hybrids: Linkage between Human Lactate Dehydrogenase A and B and Peptidase B

Nature ◽  
1970 ◽  
Vol 227 (5255) ◽  
pp. 251-257 ◽  
Author(s):  
F. H. RUDDLE ◽  
V. M. CHAPMAN ◽  
T. R. CHEN ◽  
R. J. KLEBE
Keyword(s):  
Lactate Dehydrogenase ◽  
Genetic Analysis ◽  
Somatic Cell ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Mouse Somatic Cell

scholarly journals GENETIC CONTROL OF MITOCHONDRIAL THYMIDINE KINASE IN HUMAN-MOUSE AND MONKEY-MOUSE SOMATIC CELL HYBRIDS

1974 ◽  
Vol 61 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Saul Kit ◽  
Wai-Choi Leung
Keyword(s):  
Somatic Cell ◽  
Thymidine Kinase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Molecular Forms ◽  
Kidney Cells ◽  
Somatic Cell Hybrids ◽  
Human Embryonic Lung ◽  
Cell Hybrids ◽  
Electrophoretic Mobilities ◽  
Mouse Somatic Cell

Distinctive thymidine (dT) kinase molecular forms are present in mouse, human, and monkey mitochondria. Disk polyacrylamide gel electrophoresis (disk PAGE) analyses have shown that the mitochondrial-specific dT kinases differ from cytosol dT kinases in relative electrophoretic mobilities (Rm). Furthermore, the mouse mitochondrial dT kinase differs in Rm value from primate mitochondrial dT kinases. The mouse and primate cytosol dT kinases can also be distinguished. Disk PAGE analyses have been carried out on the cytosol and mitochondrial dT kinases of human-mouse (WIL-8) and monkey-mouse (mK·CVIII) somatic cell hybrids in order to learn whether the mitochondria of the hybrid cells contained murine mitochondrial-specific, primate mitochondrial-specific, or both dT kinases. WIL-8 cells were derived from cytosol dT kinase-negative, mitochondrial dT kinase-positive mouse fibro blasts and from cytosol dT kinase-positive, mitochondrial dT kinase-positive human embryonic lung cells; they contained mostly mouse chromosomes and a few human chromosomes, including the determinant for human cytosol dT kinase. The mK·CVIII cells were derived from cytosol dT kinase-negative, mitochondrial dT kinase-positive mouse kidney cells and from cytosol dT kinase-positive, mitochondrial dT kinase-positive monkey kidney cells; they contained mostly mouse chromosomes and a few monkey chromosomes, including the determinant for monkey cytosol dT kinase. Disk PAGE analyses demonstrated that the mitochondria of human-mouse and monkey-mouse somatic cell hybrids contained the mouse-specific mitochondrial dT kinase but not the human- or monkey-specific mitochondrial dT kinase. These findings suggest that primate cytosol and mitochondrial thymidine kinase genes are coded on different chromosomes.

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