Quantitative application of RNA-DNA hybridization in situ

1976 ◽  
Vol 17 (3) ◽  
pp. 137-143 ◽  
Author(s):  
D.J. Wolgemuth-Jarashow ◽  
G.M. Jagiello ◽  
K.C. Atwood ◽  
A.S. Henderson
Keyword(s):  
Dna Hybridization ◽  
Hybridization In Situ

Localization of the 5S RNA genes in Zea mays by RNA-DNA hybridization in situ

Chromosoma ◽  
1974 ◽  
Vol 47 (4) ◽  
pp. 353-359 ◽  
Author(s):  
D. E. Wimber ◽  
Patricia A. Duffey ◽  
D. M. Steffensen ◽  
W. Prensky
Keyword(s):  
Zea Mays ◽  
Dna Hybridization ◽  
5S Rna ◽  
Hybridization In Situ ◽  
5S Rna Genes ◽  
Rna Genes

Comparison of the DNA of nuclei of rodlet cells and other cells in the chub Semotilus atromaculatus: hybridization in situ

1986 ◽  
Vol 64 (4) ◽  
pp. 801-804 ◽  
Author(s):  
D. L. Barber ◽  
J. E. Mills Westermann
Keyword(s):  
Dna Hybridization ◽  
Genomic Dna ◽  
Cell Types ◽  
Semotilus Atromaculatus ◽  
Different Temperatures ◽  
Hybridization In Situ ◽  
The Stability ◽  
Creek Chub ◽  
Rodlet Cells

With nick-translated genomic DNA of the northern creek chub, Semotilus atromaculatus, DNA–DNA hybridization of rodlet cells and cells known to belong to this species showed the same degree of labeling in the nuclei. The amount of hybridization for all cells was greater than that seen in chub cells treated with rainbow trout genomic DNA and than that found when other teleost, amphibian, human, or protozoan cells were treated with chub genomic DNA. Washing experiments at different temperatures also showed that the stability of the hybrids was comparable for both cell types. As a result, the nuclei of rodlet cells in this species are considered to contain qualitatively the same DNA as cells known to belong to the fish, and the rodlet cell itself, therefore, is of teleost origin.

A new method for the in situ assay of α-glucosidase activity and inhibitor screening through an enzyme substrate-mediated DNA hybridization chain reaction

2019 ◽  
Vol 43 (24) ◽  
pp. 9458-9465
Author(s):  
Xiquan Yue ◽  
Lihong Su ◽  
Xu Chen ◽  
Junfeng Liu ◽  
Longpo Zheng ◽  
...  
Keyword(s):  
Small Molecule ◽  
Dna Hybridization ◽  
New Method ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Inhibitor Screening ◽  
Glucosidase Activity ◽  
Enzyme Substrate ◽  
In Situ Assay

The strategy is based on small molecule-mediated hybridization chain reaction.

scholarly journals Human megakaryocytes express clusterin and package it without apolipoprotein A-1 into alpha-granules

Blood ◽  
1993 ◽  
Vol 82 (1) ◽  
pp. 118-125 ◽  
Author(s):  
J Tschopp ◽  
DE Jenne ◽  
S Hertig ◽  
KT Preissner ◽  
H Morgenstern ◽  
...  
Keyword(s):  
Tissue Injury ◽  
Density Lipoprotein ◽  
Blood Pool ◽  
Human Platelets ◽  
Blood Leukocytes ◽  
Apolipoprotein A ◽  
Hybridization In Situ ◽  
Plasma Glycoprotein ◽  
Clusterin Expression

Clusterin, a 70-Kd disulfide-linked two-chain plasma glycoprotein circulates in blood as a high-density lipoprotein particle and is highly induced after tissue injury and tissue remodeling. In this study, peripheral blood leukocytes were assayed for clusterin expression. The protein was predominantly detectable in human platelets by immune cytochemistry. The content of clusterin was determined and amounts to 2.5 +/- 1.3 micrograms/10(9) platelets, thus representing about 2% of the blood pool. Clusterin purified from human platelets had the same molecular weight as plasma clusterin under nonreducing conditions and was composed of two disulfide-linked nonidentical subunits of the same size. Both preparations were sensitive to reduction yielding the two subunits of 35 Kd. In contrast to plasma clusterin, the platelet form was not complexed to apolipoprotein A-I. By immunogold labeling, alpha-granule localization of clusterin was observed. Complete release of platelet clusterin occurred at optimal doses of A23187, phorbol myristate acetate (PMA), and thrombin. Because clusterin mRNA was detected by hybridization in situ in bone marrow- derived megakaryocytes, platelet clusterin is most likely produced and packaged into alpha-granules during megakaryocyte development.

Detection of C. Trachomatis by in Situ DNA Hybridization: Report of Two Cases with Neonatal Pneumonia

1990 ◽  
Vol 32 (3) ◽  
pp. 319-322 ◽  
Author(s):  
.Makiko Ohyama ◽  
Yukichi Tanaka ◽  
Yoshiro Sasaki ◽  
Akiko Goto
Keyword(s):  
Dna Hybridization

scholarly journals FEATURES OF VIOLATIONS OF THE STATE OF THE VAGINAL ECOSYSTEM IN PREGNANT WOMEN WITH BACTERIAL VAGINOSIS

2021 ◽  
Vol 74 (3) ◽  
pp. 460-464
Author(s):  
Iryna M. Shcherbina ◽  
Iryna Yu. Plakhotna
Keyword(s):  
Pregnant Women ◽  
Bacterial Resistance ◽  
Main Group ◽  
Vaginal Smears ◽  
Dna Diagnostics ◽  
Hybridization In Situ ◽  
Positive Hybridization ◽  
Main Component ◽  
Microbial Spectrum

The aim: To assess the condition of the vaginal ecosystem in pregnant women with BV. Materials and methods: The main group consisted of 60 pregnant women with BV in the II trimester. The bacterioscopic examination, of vaginal smears was carried out. DNA diagnostics of the microbial spectrum of vaginal contents was performed. Bacteria with biofilm were visualized by fluorescence hybridization in situ. Results: Biofilms were found in 25 women (41.65%) of the main group, the main component of which was bacteria belonging to the Gardnerella cluster at a concentration of 7.9 ± 0.13 log CFU/ g. Atopobium vagine cluster bacteria gave positive hybridization signals in more than half of the patients and amounted to 6.8 ± 0.15 lg CFU / g. In addition, Snethia spp. was determined as a part of the biofilm at a concentration of 5.8 ± 0.3 lg CFU / g. Conclusions: Thus, the use of the proposed treatment regimen for women with vaginal dysbiosis led to the elimination of pathogenic and conditionally pathogenic microflora. However, the effectiveness of treatment in 5 cases was lower than expected, which indicates the emergence of bacterial resistance.

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