Comparison of the DNA of nuclei of rodlet cells and other cells in the chub Semotilus atromaculatus: hybridization in situ

1986 ◽  
Vol 64 (4) ◽  
pp. 801-804 ◽  
Author(s):  
D. L. Barber ◽  
J. E. Mills Westermann
Keyword(s):  
Dna Hybridization ◽  
Genomic Dna ◽  
Cell Types ◽  
Semotilus Atromaculatus ◽  
Different Temperatures ◽  
Hybridization In Situ ◽  
The Stability ◽  
Creek Chub ◽  
Rodlet Cells

With nick-translated genomic DNA of the northern creek chub, Semotilus atromaculatus, DNA–DNA hybridization of rodlet cells and cells known to belong to this species showed the same degree of labeling in the nuclei. The amount of hybridization for all cells was greater than that seen in chub cells treated with rainbow trout genomic DNA and than that found when other teleost, amphibian, human, or protozoan cells were treated with chub genomic DNA. Washing experiments at different temperatures also showed that the stability of the hybrids was comparable for both cell types. As a result, the nuclei of rodlet cells in this species are considered to contain qualitatively the same DNA as cells known to belong to the fish, and the rodlet cell itself, therefore, is of teleost origin.

scholarly journals A Superhydrophobic, Antibacterial, and Durable Surface of Poplar Wood

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1885
Author(s):  
Xinyu Wu ◽  
Feng Yang ◽  
Jian Gan ◽  
Zhangqian Kong ◽  
Yan Wu
Keyword(s):  
Stearic Acid ◽  
Antibacterial Properties ◽  
Alkali Resistance ◽  
Poplar Wood ◽  
X Ray Diffraction ◽  
X Ray ◽  
Different Temperatures ◽  
Acid And Alkali Resistance ◽  
The Stability

The silver particles were grown in situ on the surface of wood by the silver mirror method and modified with stearic acid to acquire a surface with superhydrophobic and antibacterial properties. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and X-ray energy spectroscopy (XPS) were used to analyze the reaction mechanism of the modification process. Scanning electron microscopy (SEM) and contact angle tests were used to characterize the wettability and surface morphology. A coating with a micro rough structure was successfully constructed by the modification of stearic acid, which imparted superhydrophobicity and antibacterial activity to poplar wood. The stability tests were performed to discuss the stability of its hydrophobic performance. The results showed that it has good mechanical properties, acid and alkali resistance, and UV stability. The durability tests demonstrated that the coating has the function of water resistance and fouling resistance and can maintain the stability of its hydrophobic properties under different temperatures of heat treatment.

Quantitative application of RNA-DNA hybridization in situ

1976 ◽  
Vol 17 (3) ◽  
pp. 137-143 ◽  
Author(s):  
D.J. Wolgemuth-Jarashow ◽  
G.M. Jagiello ◽  
K.C. Atwood ◽  
A.S. Henderson
Keyword(s):  
Dna Hybridization ◽  
Hybridization In Situ

Localization of the 5S RNA genes in Zea mays by RNA-DNA hybridization in situ

Chromosoma ◽  
1974 ◽  
Vol 47 (4) ◽  
pp. 353-359 ◽  
Author(s):  
D. E. Wimber ◽  
Patricia A. Duffey ◽  
D. M. Steffensen ◽  
W. Prensky
Keyword(s):  
Zea Mays ◽  
Dna Hybridization ◽  
5S Rna ◽  
Hybridization In Situ ◽  
5S Rna Genes ◽  
Rna Genes

Nuclear envelopes: Structure and biochemistry of the nuclear envelope

1974 ◽  
Vol 268 (891) ◽  
pp. 67-93 ◽  
Keyword(s):  
Nuclear Genome ◽  
Cell Types ◽  
Structure Lipid ◽  
Attachment Sites ◽  
Enzyme Pattern ◽  
The Stability ◽  
Pore Complexes ◽  
The Relationship ◽  
Kinetics Of

The ultrastructure of the nuclear evelope is described in various cell types with special emphasis on its pore complexes (p.c.). The architecture of the p.c. is defined against the properties of other membranous pore formations. Evidence is presented that the non-membranous p.c. components contain ribonucleoproteins but do not represent the attachment sites of nuclear chromatin. The possible dynamic nature of the p.c. material is discussed in relation to nucleocytoplasmic translocation processes. DNA of the nuclear genome is firmly attached to interporous sections of the inner nuclear membrane. The stability of this attachment is demonstrated, and chemical and conformational characteristics as well as periods and kinetics of replication are given for both isolated membrane DNA and the corresponding chromatin in situ . The membrane-associated chromatin is dominated by a heterochromatinous character; it does not represent a transitory membrane interaction of replicating DNA. It is hypothesized that membraneattachment of specific regions of the chromosomes are a means to their ordered arrangement during interphase and prophase. Structure, lipid, protein and enzyme pattern of the nuclear membranes, as well as the incorporation kinetics, underline the relationship to the endoplasmic reticulum.

scholarly journals The Implications of Fragmented Genomic DNA Size Range on the Hybridization Efficiency in NanoGene Assay

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2646 ◽  
Author(s):  
Xiaofang Wang ◽  
Beelee Chua ◽  
Ahjeong Son
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Hybridization ◽  
Genomic Dna ◽  
Size Range ◽  
Optimum Size ◽  
Hybridization Efficiency ◽  
Dna Size

DNA hybridization-based assays are well known for their ability to detect and quantify specific bacteria. Assays that employ DNA hybridization include a NanoGene assay, fluorescence in situ hybridization, and microarrays. Involved in DNA hybridization, fragmentation of genomic DNA (gDNA) is necessary to increase the accessibility of the probe DNA to the target gDNA. However, there has been no thorough and systematic characterization of different fragmented gDNA sizes and their effects on hybridization efficiency. An optimum fragmented size range of gDNA for the NanoGene assay is hypothesized in this study. Bacterial gDNA is fragmented via sonication into different size ranges prior to the NanoGene assay. The optimum size range of gDNA is determined via the comparison of respective hybridization efficiencies (in the form of quantification capabilities). Different incubation durations are also investigated. Finally, the quantification capability of the fragmented (at optimum size range) and unfragmented gDNA is compared.

The rodlet cell of Semotilus atromaculatus and Catostomus commersoni (Teleostei): studies on its identity using histochemistry and DNase I – gold, RNase A – gold, and S1 nuclease – gold labeling techniques

1986 ◽  
Vol 64 (4) ◽  
pp. 805-813 ◽  
Author(s):  
D. L. Barber ◽  
J. E. Mills Westermann
Keyword(s):  
Rnase A ◽  
Methyl Green ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
S1 Nuclease ◽  
Semotilus Atromaculatus ◽  
Left Handed ◽  
Core Boundary ◽  
Creek Chub ◽  
Rodlet Cells

Rodlet cells, enigmatic, variably present components of several teleost epithelia, have been regarded as normal cells of unknown function or parasites of unknown phylogeny. The present study examines rodlet cells in the northern creek chub, Semotilus atromaculatus, and the white sucker, Catostomus commersoni. In light microscopic histochemistry, rodlet cores give "RNA-type" reactions to acridine orange and methyl green – pyronin procedures, but in electon microscopy, application of the nuclease–gold procedure shows that rodlets contain DNA in a helical distribution at the core boundary, but not RNA. Rodlet cores also are labeled by S1 nuclease – gold, an enzyme that is specific for single-stranded DNA. We have concluded that DNA, and only DNA, is found in the rodlet, and that it occurs in a conformation not normally seen in the eukaryote nucleus. In fact, the rodlet with its DNA resembles no known eukaryote, prokaryote, or virus. Discussion includes the possibility that the rodlet core may be a natural example of DNA containing left-handed sequences (Z-DNA). Since the nucleus of the rodlet cell contains the same amount of DNA as nuclei of teleost cells, the cell itself is concluded to be of teleost origin, and the rodlets are proposed to be invasive structures of unknown phylogeny which convert the metabolism of the teleost cell to rodlet production.

Tissue- and development-specific induction and turnover of hsp70 transcripts from loci 87A and 87C after heat shock and during recovery inDrosophila melanogaster

2002 ◽  
Vol 205 (3) ◽  
pp. 345-358 ◽  
Author(s):  
S. C. Lakhotia ◽  
K. V. Prasanth
Keyword(s):  
Heat Shock ◽  
Cost Benefit ◽  
Cell Types ◽  
Specific Induction ◽  
Cost Benefit Ratio ◽  
Multiple Copies ◽  
The Stability ◽  
The Cost ◽  
Different Cell Types

SUMMARYThe haploid genome of Drosophila melanogaster normally carries at least five nearly identical copies of heat-shock-inducible hsp70 genes, two copies at the 87A7 and three copies at the 87C1 chromosome sites. We used in situ hybridization of the cDNA, which hybridizes with transcripts of all five hsp70 genes, and of two 3′ untranslated region (3′UTR; specific for the 87A7- and 87C1-type hsp70 transcripts) riboprobes to cellular RNA to examine whether all these copies were similarly induced by heat shock in different cell types of D. melanogaster. Our results revealed remarkable differences not only in the heat-shock-inducibility of the hsp70 genes at the 87A7 and 87C1 loci, but also in their post-transcriptional metabolism, such as the stability of the transcripts and of their 3′UTRs in different cell types in developing embryos and in larval and adult tissues. Our results also revealed the constitutive presence of the heat-shock-inducible form of Hsp70 in a subset of late spermatogonial cells from the second-instar larval stage onwards. We suggest that the multiple copies of the stress-inducible hsp70 genes do not exist in the genome of D. melanogaster only to produce large amounts of the Hsp70 rapidly and at short notice, but that they are specifically regulated in a developmental-stage-specific manner. It is likely that the cost/benefit ratio of not producing or of producing a defined amount of Hsp70 under stress conditions varies for different cell types and under different physiological conditions and, accordingly, specific regulatory mechanisms operating at the transcriptional and post-transcriptional levels have evolved.

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