Regional mapping of the gene for human lysosomal acid phosphatase (ACP2) using a hybrid clone panel containing segments of human chromosome 11

1978 ◽  
Vol 45 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Carol Jones ◽  
Fa-Ten Kao
Keyword(s):  
Acid Phosphatase ◽  
Human Chromosome ◽  
Hybrid Clone ◽  
Regional Mapping ◽  
Chromosome 11 ◽  
Lysosomal Acid

The Demonstration of Human Prostatic Acid Phosphatase (Pap) With Phosphorylcholine

1976 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
Prostatic Acid Phosphatase ◽  
Human Prostate ◽  
Rat Tissues ◽  
Specific Substrate ◽  
Acid Phosphatases ◽  
Lysosomal Acid ◽  
Cacodylate Buffer

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.

Prostatic Acid Phosphatase (PAP) Differentiated Ultracytochemically from Lysosomal Acid Phosphate in the Rat with a PAP/Specific Substrate, Phosphorylcholine

1975 ◽  
Vol 33 ◽  
pp. 450-451
Author(s):  
W. Allen Shannon ◽  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Phosphate Buffer ◽  
Prostatic Carcinoma ◽  
Prostatic Acid Phosphatase ◽  
Chemotherapeutic Agents ◽  
Specific Substrate ◽  
Acid Phosphate ◽  
Lysosomal Acid ◽  
Design And Synthesis ◽  
New Chemotherapeutic Agents

During the design and synthesis of new chemotherapeutic agents for prostatic carcinoma based on phosphorylated agents which might be enzyme-activated to cytotoxicity, phosphorylcholine, [(CH3)3+NCH2CH2OPO3Ca]Cl-, has been indicated to be a very specific substrate for prostatic acid phosphatase (PAP). This phenomenon has led to the development of specific histochemical and ultracytochemical methods for PAP using modifications of the Gomori lead method for acid phosphatase. Comparative histochemical results in prostate and kidney of the rat have been published earlier with phosphorylcholine (PC) and β-glycerophosphate (βGP). We now report the ultracytochemical results.Minced tissues were fixed in 3% glutaraldehyde-0.1 M phosphate buffered (pH 7.4) for 1.5 hr and rinsed overnight in several changes of 0.05 M phosphate buffer (pH 7.0) containing 7.5% sucrose. Tissues were incubated 30 min to 2 hr in Gomori acid phosphatase medium (2) containing 0.1 M substrate, either PC or βGP.

Irradiation Hybrids for Human Chromosome 11: Characterization and Use for Generating Region-Specific Markers in 11q14-q23

Genomics ◽  
1993 ◽  
Vol 15 (2) ◽  
pp. 332-341 ◽  
Author(s):  
G.T. Gillett ◽  
C.M. McConville ◽  
P.J. Byrd ◽  
T. Stankovic ◽  
A.M. Taylor ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 11

The Gene Encoding Protein Kinase SEK1 Maps to Mouse Chromosome 11 and Human Chromosome 17

Genomics ◽  
1996 ◽  
Vol 34 (3) ◽  
pp. 430-432 ◽  
Author(s):  
Robert A. White ◽  
Rowland T. Hughes ◽  
Linda R. Adkison ◽  
Gail Bruns ◽  
Leonard I. Zon
Keyword(s):  
Protein Kinase ◽  
Human Chromosome ◽  
Mouse Chromosome ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Gene Encoding ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11 ◽  
Encoding Protein

Regional mapping of a liver α-subunit gene of phosphorylase kinase (PHKA) to the distal region of human chromosome Xp

1992 ◽  
Vol 60 (3-4) ◽  
pp. 194-196 ◽  
Author(s):  
J.G. Wauters ◽  
P.J. Bossuyt ◽  
J. Davidson ◽  
J. Hendrickx ◽  
M.W. Kilimann ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Distal Region ◽  
Alpha Subunit ◽  
Phosphorylase Kinase ◽  
Regional Mapping ◽  
Subunit Gene

High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones

Science ◽  
1990 ◽  
Vol 247 (4938) ◽  
pp. 64-69 ◽  
Author(s):  
P Lichter ◽  
C. Tang ◽  
K Call ◽  
G Hermanson ◽  
G. Evans ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Human Chromosome ◽  
Chromosome 11 ◽  
High Resolution Mapping ◽  
Resolution Mapping

High Degree of Homology Between Primary Structure of Human Lysosomal Acid Phosphatase and Human Prostatic Acid Phosphatase

1989 ◽  
Vol 370 (1) ◽  
pp. 177-182 ◽  
Author(s):  
Christoph PETERS ◽  
Carola GEIER ◽  
Regina POHLMANN ◽  
Abdul WAHEED ◽  
Kurt VON FIGURA ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Primary Structure ◽  
Prostatic Acid Phosphatase ◽  
Lysosomal Acid ◽  
High Degree
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