Secondary Constrictions in Human Chromosomes

1965 ◽  
Vol 4 (4-5) ◽  
pp. 261-276 ◽  
Author(s):  
Catherine G. Palmer ◽  
Sandra Funderburk
Keyword(s):  
Human Chromosomes ◽  
Secondary Constrictions

GIEMSA BANDING OF THE CHROMOSOMES OF THE DOMESTIC SHEEP (OVIS ARIES)

1974 ◽  
Vol 16 (3) ◽  
pp. 555-564 ◽  
Author(s):  
D. L. Zartman ◽  
A. N. Bruère
Keyword(s):  
Tritiated Thymidine ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Human Chromosomes ◽  
Giemsa Banding ◽  
Chromosomal Dna ◽  
X Chromosomes ◽  
Standard Of Comparison ◽  
Naoh Treatment ◽  
Secondary Constrictions

A Giemsa banding procedure was used to construct a basic G-band idiogram for the domestic sheep. The idiogram is labelled in a systematic manner according to the routine recommended for human chromosomes. This pattern based on NaOH treatment, provides a standard of comparison for further studies on intra- and interspecific chromosome homologies in addition to identification of chromosomal abnormalities.Late replicating regions of chromosomal DNA were detected with tritiated thymidine. Partial homologies between G-bands and these late replicating areas were found. Previously reported areas of prevalent secondary constrictions were seen to coincide with late replicating, G-positive regions on the metacentric and X chromosomes.

THE DISTRIBUTION AND LOCALIZATION OF DRUG-INDUCED SECONDARY CONSTRICTIONS IN HUMAN CHROMOSOMES

1972 ◽  
Vol 14 (1) ◽  
pp. 81-93 ◽  
Author(s):  
J. A. Brown ◽  
C. G. Palmer ◽  
P. L. Yu
Keyword(s):  
Dna Synthesis ◽  
Mitomycin C ◽  
Hydroxylamine Hydrochloride ◽  
Human Chromosomes ◽  
Chromosome Identification ◽  
Drug Induced ◽  
Drug Dependent ◽  
Secondary Constrictions

BUdR, mitomycin C and hydroxylamine hydrochloride induce secondary constrictions in particular human chromosomes. The increased frequency of constriction formation in certain chromosomes is time and drug dependent, and correlates well with the timing of DNA synthesis. The frequency at which some of these constrictions can be induced by specific drugs at specific times increases the usefulness of these landmarks in chromosome identification.

5-BROMODEOXYURIDINE-INDUCED CONSTRICTIONS IN HUMAN CHROMOSOMES

1970 ◽  
Vol 12 (4) ◽  
pp. 816-830 ◽  
Author(s):  
Catherine G. Palmer
Keyword(s):  
Sex Chromosomes ◽  
Chromosomal Abnormalities ◽  
Human Chromosomes ◽  
Human Complement ◽  
Characteristic Morphology ◽  
Secondary Constrictions

BUdR treatment during the last 5 to 7 hours of growth of leukocyte cultures significantly increases frequency of all secondary constrictions and produces characteristic morphology in certain chromosomes of the human complement. BUdR-induced morphology of the D group and sex chromosomes is described and specific patterns are related to specific chromosomes by the use of identifiable chromosomal abnormalities.

A role for upstream binding factor in organizing ribosomal gene chromatin

2006 ◽  
Vol 73 ◽  
pp. 77-84 ◽  
Author(s):  
Jane E. Wright ◽  
Christine Mais ◽  
José-Luis Prieto ◽  
Brian McStay
Keyword(s):  
Nucleolar Organizer ◽  
Ribosomal Gene ◽  
Rna Polymerase I ◽  
Nucleolar Organizer Regions ◽  
Binding Factor ◽  
Upstream Binding Factor ◽  
Acrocentric Chromosomes ◽  
Polymerase I ◽  
Secondary Constrictions ◽  
Active Nors

Human ribosomal genes are located in NORs (nucleolar organizer regions) on the short arms of acrocentric chromosomes. During metaphase, previously active NORs appear as prominent chromosomal features termed secondary constrictions, which are achromatic in chromosome banding and positive in silver staining. The architectural RNA polymerase I transcription factor UBF (upstream binding factor) binds extensively across the ribosomal gene repeat throughout the cell cycle. Evidence that UBF underpins NOR structure is provided by an examination of cell lines in which large arrays of a heterologous UBF binding sequences are integrated at ectopic sites on human chromosomes. These arrays efficiently recruit UBF even to sites outside the nucleolus, and during metaphase form novel silver-stainable secondary constrictions, termed pseudo-NORs, that are morphologically similar to NORs.

Automatic Karyotyping of Human Chromosomes Using Band Patterns

2012 ◽  
Vol 2 (12) ◽  
pp. 154-156 ◽  
Author(s):  
Priya Chaku ◽  
◽  
Pooja Shah
Keyword(s):  
Human Chromosomes

Linkage of genetic markers on human chromosomes 20 and 12 to NIDDM in Caucasian sib pairs with a history of diabetic nephropathy

Diabetes ◽  
1997 ◽  
Vol 46 (5) ◽  
pp. 882-886 ◽  
Author(s):  
D. W. Bowden ◽  
M. Sale ◽  
T. D. Howard ◽  
A. Qadri ◽  
B. J. Spray ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Genetic Markers ◽  
Human Chromosomes ◽  
History Of ◽  
Sib Pairs
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