Opposite Effects of H2-Receptors on Parietal Cells and Chief Cells

1984 ◽  
Vol 16 (2) ◽  
pp. 55-61 ◽  
Author(s):  
I. Guldvog ◽  
A. Berstad
Keyword(s):  
Parietal Cells ◽  
Chief Cells

scholarly journals Comparative Histological Study of the Stomach in Two Species of Iraqi Vertebrates (Magpie Pica pica L. and Small Asian Mongoose Herpestes javanicus E.)

2019 ◽  
Vol 16 (2) ◽  
pp. 0281
Author(s):  
AL –Nakeeb Et al.
Keyword(s):  
Outer Layer ◽  
Histological Study ◽  
Gastric Gland ◽  
Parietal Cells ◽  
Pica Pica ◽  
Gastric Glands ◽  
Chief Cells ◽  
Herpestes Javanicus ◽  
Mucous Neck Cells ◽  
Muscularis Externa

          A histological study showed the wall of the stomach in Pica pica and Herpestes javanicus consists of four layers: mucosa, submucosa, muscularis externa and serosa. Also, the present study showed many  differences in the histological structures of the stomach for each in both types. The stomach of P. pica consists of two portions: the proventiculus and gizzard, while the stomach of H. javanicus consists of three portions: cardiac, fundic and pyloric regions. The mucosa layer formed short gastric folds, named plicae. In the proventiculus of P. pica, sulcus is found between each two plicae, but the folds called gastric pits in the gizzard, which are full with koilin. Lamina properia in both types contained gastric glands (straight simple tubular glands) named superficial glands, as well as another gastric gland found in the submucosa layer of the proventiculus in P. pica only named deep gastric glands. The gastric gland in the stomach of H. javanicus contained: mucous neck cells and parietal cells positive to AB/PAS stains in cardiac portion, as well as chief cells in fundic portion, but pyloric portion had just mucous neck cells. Muscularis externa in both types formed two muscle layers: inner and outer layer.

Renewal of parietal cells, chief cells, and smooth muscle cells in mouse stomach

1998 ◽  
Vol 114 ◽  
pp. A893
Author(s):  
Y. Magami ◽  
S. Kokuno ◽  
M. Furukawa ◽  
Y. Tsukioka ◽  
D. Nakayama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Parietal Cells ◽  
Chief Cells

Anatomical location of enterochromaffin-like (ECL) cells, parietal cells, and chief cells in the stomach demonstrated by immunocytochemistry and electron microscopy

1999 ◽  
Vol 34 (3) ◽  
pp. 315-320 ◽  
Author(s):  
Sachiko Kamoshida ◽  
Eiichi Saito ◽  
Satoru Fukuda ◽  
Kimitoshi Kato ◽  
Ariyoshi Iwasaki ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Parietal Cells ◽  
Anatomical Location ◽  
Chief Cells ◽  
Ecl Cells

Role of vacuolation in the death of gastric epithelial cells

1997 ◽  
Vol 272 (1) ◽  
pp. C48-C58 ◽  
Author(s):  
S. J. Hagen ◽  
S. Takahashi ◽  
R. Jansons
Keyword(s):  
Epithelial Cells ◽  
Cell Survival ◽  
Potent Inhibitor ◽  
Chief Cell ◽  
Parietal Cells ◽  
Gastric Epithelial Cells ◽  
Bafilomycin A1 ◽  
Gastric Glands ◽  
Chief Cells

The effect of vacuolation on survival of gastric epithelial cells was studied in rabbit gastric glands (RGG) incubated with ammonia and bafilomycin A1, a potent inhibitor of vacuolar ATPase activity. In ammonia, large vacuoles formed and cell survival was reduced to 47.2 +/- 3.4% at 6 h (59.5 +/- 3.8%, buffer). Bafilomycin A1 added at the start to RGG incubated with ammonia inhibited vacuole formation but did not improve cell survival (48.7 +/- 2.8% at 6 h). Bafilomycin A1 added 1-2 h after addition of ammonia reduced the size of vacuoles but did not alter cell survival. Cell survival was not affected by inhibiting protein synthesis. When incubated with ammonia, parietal cells dissociated from the gland and ruptured. After this, chief cells condensed and formed expensive blebs that contained fragmented nuclei. We conclude that 1)ammonia-induced vacuolation of gastric epithelial cells does not influence cell survival, 2) ammonia facilitates necrosis in parietal cells and apoptosis in chief cells, and 3) chief cell survival, in some manner, may be dependent on parietal cells.

Expression of intrinsic factor and pepsinogen in the rat stomach identifies a subset of parietal cells

1998 ◽  
Vol 274 (1) ◽  
pp. G62-G70 ◽  
Author(s):  
J.-S. Shao ◽  
W. Schepp ◽  
D. H. Alpers
Keyword(s):  
Gastric Gland ◽  
Intrinsic Factor ◽  
Cell Lineage ◽  
Neck Region ◽  
Chief Cell ◽  
Parietal Cells ◽  
Rat Stomach ◽  
Chief Cells ◽  
Indirect Immunoperoxidase ◽  
Lower Neck

Morphological and functional heterogeneity of parietal cells has been thought to be due to different maturation positions within the gastric gland. Morphodynamic studies have shown that 2% of parietal cells in mice derive from a pre-neck (chief) cell precursor. Intrinsic factor (IF) and pepsinogen, markers of rat chief cells, were used to determine if these proteins identified a subset of parietal cells that might reflect origin from the pre-neck cell lineage. The zymogenic region of the rat stomach and gradient-isolated fractions enriched in parietal and chief cells were fixed in 10% buffered Formalin or in Bouin’s solution. Immunostaining was performed using indirect immunoperoxidase histochemistry and double-labeled immunofluorescence with antibodies raised against human IF, pepsinogen II, and H+-K+-adenosinetriphosphatase (H+-K+-ATPase). In intact tissue, parietal (H+-K+-ATPase-positive) cells were found starting at the upper edge of the isthmus, but parietal cells positive for IF and pepsinogen were only found from just below the isthmus and neck region to the base of the gastric gland. Three to four percent of isolated parietal cells were positive for these ectopic markers. This subset of cells was also positive for H+-K+-ATPase. Thus products of rat chief cells are expressed in a subset of parietal cells. The percentage of positive cells is similar to that predicted to be derived from the pre-neck (chief) precursor lineage in the mouse. The distribution of these cells to the lower neck and base of the gland suggests that the expression of chief cell products is consistent with either predetermination by lineage or parietal cell maturation or with both processes.

Inositol phosphate formation and [Ca2+]i in secretagogue-stimulated rabbit gastric mucous cells

1991 ◽  
Vol 260 (1) ◽  
pp. G133-G141 ◽  
Author(s):  
U. Seidler ◽  
A. Pfeiffer
Keyword(s):  
Inositol Phosphates ◽  
Inositol Phosphate ◽  
Mucous Cell ◽  
Parietal Cells ◽  
Secretory Cells ◽  
Rapid Decline ◽  
Mucous Cells ◽  
Chief Cells ◽  
Gastric Mucous ◽  
Stimulation Of

The formation of inositol phosphates and the changes in free intracellular Ca2+ ([Ca2+]i) in isolated rabbit gastric mucous cells during cholinergic stimulation were examined and the potential role of inositol phosphate turnover and [Ca2+]i in gastric mucus secretion evaluated. Rabbit chief and parietal cells were studied for comparison. The formation of [3H]inositol phosphates in mucous, chief, and parietal cells was stimulated in a time- and concentration-dependent fashion by acetylcholine (ACh). The ACh-induced initial [Ca2+]i peak was maximally (10(-4) M ACh) 199 +/- 8% of basal in mucous cells, 427 +/- 20% in chief, and 455 +/- 31% in parietal cells and was followed by a lower-level plateau in mucous and parietal cells but by a more rapid decline in chief cells. As in parietal and chief cells, the initial [Ca2+]i peak occurred in mucous cells in the absence of external Ca2+. ACh stimulated a mucous cell membrane Ca2(+)-entry mechanism in addition to release of Ca2+ from intracellular stores. The concentration-response relationships for the production of [3H]-inositol phosphates, the initial rise in [Ca2+]i, and the stimulation of glycoprotein secretion by ACh were virtually identical. Suppression of the [Ca2+]i rise by the intracellular Ca2(+)-chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) abolished the secretory response. As with many other secretory cells, gastric mucous cells possess cholinergic receptors that upon stimulation mediate the hydrolysis of phosphoinositides, a release of Ca2+ from intracellular stores, and a stimulation of Ca2+ influx through the plasma membrane.

Comparative morphology of the stomach of some laboratory mammals

1989 ◽  
Vol 23 (1) ◽  
pp. 21-29 ◽  
Author(s):  
N. G. Ghoshal ◽  
H. S. Bal
Keyword(s):  
Lamina Propria ◽  
Gradual Increase ◽  
Comparative Morphology ◽  
Parietal Cells ◽  
Glandular Stomach ◽  
Structural Basis ◽  
Cell Type ◽  
Gastric Glands ◽  
Chief Cells ◽  
Predominant Cell Type

Histomorphology of the stomach of mouse, rat, hamster, guineapig, gerbil, and rabbit was studied. Although a common structural basis existed in the stomach between these species, the occurrence and distribution of various cells in gastric glands differed considerably between them. In mice, rats, hamsters and gerbils, the lower one-third of the glandular lamina propria was seemingly occupied by a varying proportion of parietal and chief cells. In rabbits, the predominantly occurring chief cells were distributed in the lower three-quarters of the glands intermingling with parietal cells, but in guineapigs the chief cells were not discernible. In hamsters, there was, however, a gradual increase of chief cells from the junction between nonglandular-glandular stomach toward the pyloric region. In all these species, parietal cells were the predominant cell type in the upper half to upper one-third of the gastric glands, often extending up to the neck of the glands interspersing between mucus neck cells and occasionally between chief cells.

Rat parietal cell receptors for GLP-1-(7-36) amide: northern blot, cross-linking, and radioligand binding

1994 ◽  
Vol 267 (3) ◽  
pp. G423-G432 ◽  
Author(s):  
J. Schmidtler ◽  
K. Dehne ◽  
H. D. Allescher ◽  
V. Schusdziarra ◽  
M. Classen ◽  
...  
Keyword(s):  
Parietal Cell ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Parietal Cells ◽  
Small Cells ◽  
Cross Linking ◽  
Cell Content ◽  
Chief Cells ◽  
High Affinity ◽  
Isolated Rat

The intestinal peptide hormone glucagon-like peptide-1 (GLP-1) (7-36) amide is a potent stimulus of H+ production in isolated rat parietal cells, suggesting the presence of specific GLP-1-receptors on this cell type. Our aim was to characterize these receptors. Enzymatically isolated rat gastric mucosal cells (F0) were fractionated by counterflow elutriation, resulting in five fractions (F1-F5) according to increasing cell diameter and parietal cell content (3, 5, 4, 27, 81%). Additional density gradient centrifugation of F4 yielded enriched chief cells (74%; parietal cells: 1%; F6), whereas density gradient centrifugation of F5 almost purified parietal cells (97%; chief cells: 1%; F7). Northern blot of total cellular RNA from F0-F7 with a probe specific for the GLP-1-(7-36) amide receptor revealed two RNA species of 2.7 and 3.6 kb. These messages were present to some extent in small cells (F1, F2), much more pronounced in F5, abundant in F7, barely detectable in F3 and F4, and absent from F6. Cross-linking of 125I-labeled GLP-1-(7-36) amide to parietal cell membranes revealed a single 59-kDa band that was abolished by unlabeled GLP-1-(7-36) amide. Throughout fractions F1-F7 specific binding of 125I-GLP-1-(7-36) amide was correlated with parietal cell content (r = 0.99; P < 0.01) and H+ production ([14C]aminopyrine accumulation) in response to GLP-1-(7-36) amide or histamine (r = 0.96; P < 0.01). Binding was maximal in purified parietal cells (F7), whereas almost no binding was detectable in enriched chief cells (F6). In F7, Scatchard analysis revealed a single class of high-affinity binding sites (KD = 2.8 +/- 0.6 x 10(-10) M, Bmax = 6.8 +/- 1.4 fmol/10(6) cells, 4,096 +/- 793 receptors/parietal cells). The following half-maximal inhibition values were found for GLP-1-(7-36) amide and (1-37) and (1-36) amide: 6.6 +/- 0.9 x 10(-10), 1.4 +/- 0.7 x 10(-7), and 2.6 +/- 0.4 x 10(-7) M, respectively. Pancreatic glucagon, GLP-2, and oxyntomodulin, products of the proglucagon gene, were 3-4 log units less potent displacers while gastric inhibitory peptide, vasoactive intestinal peptide, and secretin were ineffective. We conclude that rat parietal cells are equipped with specific high-affinity receptors for GLP-1-(7-36) amide, which, in addition, are present in as yet unidentified small cells (F1, F2) but not in chief cells.

scholarly journals Fluorescence measurements of cytosolic free Na concentration, influx and efflux in gastric cells.

1990 ◽  
Vol 1 (3) ◽  
pp. 259-268 ◽  
Author(s):  
P A Negulescu ◽  
A Harootunian ◽  
R Y Tsien ◽  
T E Machen
Keyword(s):  
Cell Types ◽  
Parietal Cells ◽  
Acetoxymethyl Ester ◽  
Gastric Glands ◽  
Great Ability ◽  
Chief Cells ◽  
Fluorescence Measurements ◽  
Na Efflux ◽  
Rapid Perfusion ◽  
Acid Secreting

Regulation of cytosolic free Na (Nai) was measured in isolated rabbit gastric glands with the use of a recently developed fluorescent indicator for sodium, SBFI. Intracellular loading of the indicator was achieved by incubation with an acetoxymethyl ester of the dye. Digital imaging of fluorescence was used to monitor Nai in both acid-secreting parietal cells and enzyme-secreting chief cells within intact glands. In situ calibration of Nai with ionophores indicated that SBFI fluorescence (345/385 nm excitation ratio) could resolve 2 mM changes in Nai and was relatively insensitive to changes in K or pH. Measurements on intact glands showed that basal Nai was 8.5 +/- 2.2 mM in parietal cells and 9.2 +/- 3 mM in chief cells. Estimates of Na influx and efflux were made by measuring rates of Nai change after inactivation or reactivation of the Na/K ATPase in a rapid perfusion system. Na/K ATPase inhibition resulting from the removal of extracellular K (Ko) caused Nai to increase at 3.2 +/- 1.5 mM/min and 3.5 +/- 2.7 mM/min in parietal and chief cells, respectively. Na buffering was found to be negligible. Addition of 5 mM Ko and removal of extracellular Na (Nao) caused Nai to decrease rapidly toward 0 mM Na. By subtracting passive Na efflux under these conditions (the rate at which Nai decreased in Na-free solution containing ouabain), an activation curve (dNai/Nai) for the Na/K ATPase was calculated. The pump demonstrated the greatest sensitivity between 5 and 20 mM Nai. At 37 degrees C the pump rate was less than 3 mM/min at 5 mM Nai and 26 mM/min at 25 mM Nai, indicating that the pump has a great ability to respond to changes in Nai in this range. Carbachol, which stimulates secretion from both cell types, was found to stimulate Na influx in both cell types, but did not have detectable effects on Na efflux. dbcAMP+IBMX, potent stimulants of acid secretion, had no effect on Na metabolism.

Sign in / Sign up

Export Citation Format

Share Document