Anatomical location of enterochromaffin-like (ECL) cells, parietal cells, and chief cells in the stomach demonstrated by immunocytochemistry and electron microscopy

1999 ◽  
Vol 34 (3) ◽  
pp. 315-320 ◽  
Author(s):  
Sachiko Kamoshida ◽  
Eiichi Saito ◽  
Satoru Fukuda ◽  
Kimitoshi Kato ◽  
Ariyoshi Iwasaki ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Parietal Cells ◽  
Anatomical Location ◽  
Chief Cells ◽  
Ecl Cells

The effects of rabeprazole on ECL cells and parietal cells in rat -comparison with omeprazole

2000 ◽  
Vol 118 (4) ◽  
pp. A1110
Author(s):  
Akira Tari ◽  
Masanori Kawano ◽  
Toyohiko Aoki ◽  
Yoshikazu Yonei ◽  
Kanji Kodama ◽  
...  
Keyword(s):  
Parietal Cells ◽  
Ecl Cells

Opposite Effects of H2-Receptors on Parietal Cells and Chief Cells

1984 ◽  
Vol 16 (2) ◽  
pp. 55-61 ◽  
Author(s):  
I. Guldvog ◽  
A. Berstad
Keyword(s):  
Parietal Cells ◽  
Chief Cells

scholarly journals Comparative Histological Study of the Stomach in Two Species of Iraqi Vertebrates (Magpie Pica pica L. and Small Asian Mongoose Herpestes javanicus E.)

2019 ◽  
Vol 16 (2) ◽  
pp. 0281
Author(s):  
AL –Nakeeb Et al.
Keyword(s):  
Outer Layer ◽  
Histological Study ◽  
Gastric Gland ◽  
Parietal Cells ◽  
Pica Pica ◽  
Gastric Glands ◽  
Chief Cells ◽  
Herpestes Javanicus ◽  
Mucous Neck Cells ◽  
Muscularis Externa

          A histological study showed the wall of the stomach in Pica pica and Herpestes javanicus consists of four layers: mucosa, submucosa, muscularis externa and serosa. Also, the present study showed many  differences in the histological structures of the stomach for each in both types. The stomach of P. pica consists of two portions: the proventiculus and gizzard, while the stomach of H. javanicus consists of three portions: cardiac, fundic and pyloric regions. The mucosa layer formed short gastric folds, named plicae. In the proventiculus of P. pica, sulcus is found between each two plicae, but the folds called gastric pits in the gizzard, which are full with koilin. Lamina properia in both types contained gastric glands (straight simple tubular glands) named superficial glands, as well as another gastric gland found in the submucosa layer of the proventiculus in P. pica only named deep gastric glands. The gastric gland in the stomach of H. javanicus contained: mucous neck cells and parietal cells positive to AB/PAS stains in cardiac portion, as well as chief cells in fundic portion, but pyloric portion had just mucous neck cells. Muscularis externa in both types formed two muscle layers: inner and outer layer.

scholarly journals Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction

2002 ◽  
Vol 2 ◽  
pp. 1643-1645 ◽  
Author(s):  
John Graham
Keyword(s):  
Gastric Mucosa ◽  
Ion Transport ◽  
Parietal Cell ◽  
Cell Function ◽  
Parietal Cells ◽  
Centrifugal Elutriation ◽  
Ecl Cells ◽  
Acid Secreting ◽  
Density Barrier

Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation.

Renewal of parietal cells, chief cells, and smooth muscle cells in mouse stomach

1998 ◽  
Vol 114 ◽  
pp. A893
Author(s):  
Y. Magami ◽  
S. Kokuno ◽  
M. Furukawa ◽  
Y. Tsukioka ◽  
D. Nakayama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Parietal Cells ◽  
Chief Cells

Differentiation of the Gastric Mucosa I. Role of histamine in control of function and integrity of oxyntic mucosa: understanding gastric physiology through disruption of targeted genes

2006 ◽  
Vol 291 (4) ◽  
pp. G539-G544 ◽  
Author(s):  
Duan Chen ◽  
Takeshi Aihara ◽  
Chun-Mei Zhao ◽  
Rolf Håkanson ◽  
Susumu Okabe
Keyword(s):  
Gastric Mucosa ◽  
Histidine Decarboxylase ◽  
Parietal Cells ◽  
Oxyntic Mucosa ◽  
Mucosal Integrity ◽  
Ecl Cells ◽  
Gastric Physiology ◽  
Ecl Cell ◽  
Insight Into

Many physiological functions of the stomach depend on an intact mucosal integrity; function reflects structure and vice versa. Histamine in the stomach is synthesized by histidine decarboxylase (HDC), stored in enterochromaffin-like (ECL) cells, and released in response to gastrin, acting on CCK2 receptors on the ECL cells. Mobilized ECL cell histamine stimulates histamine H2 receptors on the parietal cells, resulting in acid secretion. The parietal cells express H2, M3, and CCK2 receptors and somatostatin sst2 receptors. This review discusses the consequences of disrupting genes that are important for ECL cell histamine release and synthesis (HDC, gastrin, and CCK2 receptor genes) and genes that are important for “cross-talk” between H2 receptors and other receptors on the parietal cell (CCK2, M3, and sst2 receptors). Such analysis may provide insight into the functional significance of gastric histamine.

Localization of MIWC and GLIP water channel homologs in neuromuscular, epithelial and glandular tissues

1995 ◽  
Vol 108 (9) ◽  
pp. 2993-3002 ◽  
Author(s):  
A. Frigeri ◽  
M.A. Gropper ◽  
F. Umenishi ◽  
M. Kawashima ◽  
D. Brown ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Urinary Bladder ◽  
Collecting Duct ◽  
Basolateral Membrane ◽  
Water Channel ◽  
Parietal Cells ◽  
Immunogold Electron Microscopy ◽  
Transitional Epithelium ◽  
Principal Cells ◽  
Kidney Collecting Duct

It was shown recently that water channel homologs MIWC (mercurial insensitive water channel) and GLIP (glycerol intrinsic protein) colocalized in basolateral membranes of kidney collecting duct, tracheal and colonic epithelia, and in brain pia mater. We report here an extensive immunolocalization study of MIWC and GLIP in non-epithelial and glandular epithelial tissues in rat. Immunogold electron microscopy confirmed colocalization of MIWC and GLIP in basolateral membrane of principal cells in kidney collecting duct. However, in other epithelia, MIWC but not GLIP was expressed in basolateral membrane of parietal cells in stomach, and in excretory tubules of salivary and lacrimal glands; GLIP but not MIWC was expressed in transitional epithelium of urinary bladder and skin epidermis. In the central nervous system, MIWC was strongly expressed in the ependymal layer lining the aqueductal system, and in astrocytes throughout the spinal cord and in selected regions of brain. MIWC was also expressed in a plasma membrane pattern in skeletal, but not smooth or cardiac muscle. Neither protein was expressed in small intestine, testis, liver, spleen and nerve. The tissue-specific expression of MIWC suggests a role in fluid transport and/or cell volume regulation in stomach and glandular epithelia. The functional role of MIWC expression in the neuromuscular system and of GLIP expression in skin and urinary bladder is uncertain. The specific cellular sites of MIWC expression (astrocytes, trachea, sarcolemma, gastric parietal cells and kidney principal cells) correspond exactly to sites where orthogonal arrays of particles (OAPs) have been visualized by freeze-fracture electron microscopy, suggesting that MIWC may be the OAP protein.

Role of vacuolation in the death of gastric epithelial cells

1997 ◽  
Vol 272 (1) ◽  
pp. C48-C58 ◽  
Author(s):  
S. J. Hagen ◽  
S. Takahashi ◽  
R. Jansons
Keyword(s):  
Epithelial Cells ◽  
Cell Survival ◽  
Potent Inhibitor ◽  
Chief Cell ◽  
Parietal Cells ◽  
Gastric Epithelial Cells ◽  
Bafilomycin A1 ◽  
Gastric Glands ◽  
Chief Cells

The effect of vacuolation on survival of gastric epithelial cells was studied in rabbit gastric glands (RGG) incubated with ammonia and bafilomycin A1, a potent inhibitor of vacuolar ATPase activity. In ammonia, large vacuoles formed and cell survival was reduced to 47.2 +/- 3.4% at 6 h (59.5 +/- 3.8%, buffer). Bafilomycin A1 added at the start to RGG incubated with ammonia inhibited vacuole formation but did not improve cell survival (48.7 +/- 2.8% at 6 h). Bafilomycin A1 added 1-2 h after addition of ammonia reduced the size of vacuoles but did not alter cell survival. Cell survival was not affected by inhibiting protein synthesis. When incubated with ammonia, parietal cells dissociated from the gland and ruptured. After this, chief cells condensed and formed expensive blebs that contained fragmented nuclei. We conclude that 1)ammonia-induced vacuolation of gastric epithelial cells does not influence cell survival, 2) ammonia facilitates necrosis in parietal cells and apoptosis in chief cells, and 3) chief cell survival, in some manner, may be dependent on parietal cells.

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