Sodium-, Potassium-Stimulated Adenosine Triphosphatase (Na+, K+-ATPase) Activity in Human Kidney Tissue

1973 ◽  
Vol 5 (4) ◽  
pp. 282-291 ◽  
Author(s):  
S. Pettersson ◽  
T. Scherstén
Keyword(s):  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Kidney Tissue ◽  
Human Kidney ◽  
Sodium Potassium

Effect of high salt intake on sodium, potassium-dependent adenosine triphosphatase activity in the erythrocytes of normotensive men

1988 ◽  
Vol 75 (2) ◽  
pp. 167-170 ◽  
Author(s):  
Antonio P. Quintanilla ◽  
Maria I. Weffer ◽  
Haengil Koh ◽  
Mohammed Rahman ◽  
Agostino Molteni ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Inorganic Phosphate ◽  
Adenosine Triphosphatase ◽  
Salt Intake ◽  
Control Period ◽  
Plasma Renin ◽  
Normal Diet ◽  
High Salt ◽  
Sodium Potassium ◽  
High Salt Intake

1. We measured ouabain-insensitive adenosine triphosphatase (ATPase), sodium, potassium-dependent adenosine triphosphatase (Na+,K+-ATPase) and intracellular Na+ and K+ in the erythrocytes of 19 healthy volunteers, before and after supplementation of their normal diet with 6.0–8.9 g of salt (102–137 mmol of NaCl) per day, for 5 days. 2. The subjects had a small but significant gain in weight. Mean plasma renin activity decreased from 1.57 to 0.73 pmol of angiotensin I h−1 ml−1 and plasma aldosterone from 0.46 to 0.24 nmol/l. 3. Total ATPase activity fell from 197.9 nmol of inorganic phosphate h−1 mg−1 during the control period to 173.5 during the high-salt period (P < 0.0125). Na+,K+-ATPase activity fell from 162.2 to 141.4 nmol of inorganic phosphate h−1 mg−1 (P < 0.05). Intracellular Na + and intracellular K+ did not change. 4. These results are consistent with the hypothesis that salt-induced volume expansion causes the release of a factor inhibitory to the Na+ pump.

Effect of high protein intake on sodium, potassium-dependent adenosine triphosphatase activity in the thick ascending limb of Henle's loop in the rat

1988 ◽  
Vol 74 (3) ◽  
pp. 319-329 ◽  
Author(s):  
Nadine Bouby ◽  
Lise Bankir
Keyword(s):  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Unit Length ◽  
High Protein Diet ◽  
High Protein ◽  
Salt Transport ◽  
Protein Diet ◽  
Thick Ascending Limb ◽  
Sodium Potassium ◽  
Henle's Loop

1. We have previously shown that the hypertrophy of the kidney induced by a high protein diet consists of a preferential hypertrophy of the thick ascending limb (TAL) of Henle's loop. This might be related to an increase in the active salt transport by this segment. Sodium, potassium-dependent adenosine triphosphatase (Na+,K+-ATPase) activity was measured in TAL from kidneys of rats fed either a low (LP) or a high (HP) protein diet for several weeks. 2. Enzymatic activity was measured by microdensitometry, after appropriate cytochemical reaction, for an adenosine 5′-triphosphate (ATP) concentration of 0–66 mmol/l. Both activity per unit tubular length and mean activity per unit tissue volume were recorded. A calibration was designed to convert usual microdensitometry units (extinction) into conventional biochemical units (mol of product formed). 3. For non-limiting substrate concentrations, the Na+,K+-ATPase activity, expressed per unit length of tubule on the sections, was 50% higher in HP than in LP rats, an increase proportional to that of the simultaneously measured tubule diameter. When expressed per unit tubular volume, Na+,K+-ATPase activity was similar in both groups of rats. The dissociation constant for ATP was also similar in both groups. 4. Results show that a high protein diet induces an increase in Na+,K+-ATPase activity in TAL, thus enabling an enhanced NaCl transport in this segment. This increase in transport capacity is not due to an increase in the density of enzymatic units but to an increase in their number, in relation to the hypertrophy of the TAL.

EFFECTS OF FRESHWATER ADAPTATION AND OF PROLACTIN ON SODIUM-POTASSIUMACTIVATED ADENOSINE TRIPHOSPHATASE ACTIVITY IN THE URINARY BLADDER OF TWO FLOUNDER SPECIES

1974 ◽  
Vol 62 (1) ◽  
pp. 11-14 ◽  
Author(s):  
S. UTIDA ◽  
MIE KAMIYA ◽  
D. W. JOHNSON ◽  
H. A. BERN
Keyword(s):  
Urinary Bladder ◽  
Atpase Activity ◽  
Fresh Water ◽  
Adenosine Triphosphatase ◽  
Sea Water ◽  
Sodium Potassium ◽  
Platichthys Stellatus ◽  
Freshwater Adaptation ◽  
Euryhaline Teleost ◽  
Kareius Bicoloratus

SUMMARY Sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity increased in the urinary bladder of the euryhaline teleost Platichthys stellatus after transfer from sea-water to fresh water. This increase also occurred after injection of prolactin into seawater Platichthys, simulating the results of freshwater transfer. In Kareius bicoloratus, which does not survive transfer to fresh water, prolactin does not increase bladder Na-K-ATPase activity. The differences in response of these two species to prolactin may be related to the degree of their euryhalinity. There may be a relationship between adaptability to fresh water and responsiveness of bladder Na-K-ATPase to prolactin.

Potassium adaptation and Na-K-ATPase activity in mucosa of colon

1975 ◽  
Vol 229 (6) ◽  
pp. 1576-1579 ◽  
Author(s):  
P Silva ◽  
AN Charney ◽  
FH Epstein
Keyword(s):  
Atpase Activity ◽  
Enzymatic Activity ◽  
Adenosine Triphosphatase ◽  
Adrenal Glands ◽  
Specific Activity ◽  
Sodium Potassium ◽  
Secretory Rate ◽  
Potassium Adaptation ◽  
Potassium Loading ◽  
Adrenalectomized Rats

The specific activity of sodium-potassium-activated adenosine triphosphatase in the mucosa of the colon rises when the dietary load of potassium is increased. The change in enzymatic activity depends on the presence of intact adrenal glands, since adrenalectomy abolishes the response of Na-K-ATPase to potassium loading. The increased secretory rate of aldosterone normally evoked by potassium loading appears to mediate at least in part of the effect of potassium loading, since aldosterone induces a discernible increase in the specific activity of Na-K-ATPase in the colon of adrenalectomized rats.

scholarly journals The Effect of Lithium Chloride on Renal Structure and Sodium-potassium-adenosine Triphosphatase Activity in Dogs

1982 ◽  
Vol 19 (1) ◽  
pp. 38-45 ◽  
Author(s):  
J. R. Easley
Keyword(s):  
Atpase Activity ◽  
Lithium Chloride ◽  
Inorganic Phosphate ◽  
Adenosine Triphosphatase ◽  
Distal Tubule ◽  
Distal Nephron ◽  
Sodium Potassium ◽  
Renal Structure ◽  
Collecting Ducts ◽  
Morphologic Changes

Lithium chloride was given intraperitoneally to dogs at a dosage of 125 mg/kg body weight for three days. Kidneys were removed for morphologic examination and quantitation of sodium-potassium-adenosine triphosphatase (Na-K-ATPase) activity in cortical and medullary tissue. Light microscopy showed no changes in the kidneys, but cytoplasmic vacuolation and dilatation of the cisternae of the endoplasmic reticulum were seen ultrastructurally in the epithelial cells of the distal tubule and cortical and medullary collecting ducts. Mean cortical Na-K-ATPase activity was 1.49 ± 0.25 and 1.70 ± 0.31 μmoles inorganic phosphate/mg protein/hour in control and experimental groups respectively. Mean medullary Na-K-ATPase activity was 4.71 ± 0.41 and 5.01 ± 0.47 μmoles inorganic phosphate/mg protein/hour in control and experimental groups respectively. It was concluded that lithium produced morphologic changes in the distal nephron, but had no effect on renal Na-K-ATPase activity.

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