Behaviour of sodium-potassium-activated adenosine triphosphatase in rat kidney tissue by folic acid

1969 ◽  
Vol 25 (12) ◽  
pp. 1288-1290 ◽  
Author(s):  
U. Schmidt ◽  
U. C. Dubach ◽  
J. Torhorst
Keyword(s):  
Folic Acid ◽  
Adenosine Triphosphatase ◽  
Rat Kidney ◽  
Kidney Tissue ◽  
Sodium Potassium

Circulating Inhibitor of Sodium—Potassium-Activated Adenosine Triphosphatase after Expansion of Extracellular Fluid Volume in Rats

1977 ◽  
Vol 53 (4) ◽  
pp. 329-334 ◽  
Author(s):  
H. C. Gonick ◽  
H. J. Kramer ◽  
W. Paul ◽  
E. Lu
Keyword(s):  
Inhibitory Activity ◽  
Adenosine Triphosphatase ◽  
Rat Kidney ◽  
Gel Filtration ◽  
Extracellular Fluid ◽  
Weight Fraction ◽  
Sodium Potassium ◽  
Lysine Vasopressin ◽  
Control Serum ◽  
Isotonic Sodium Chloride

1. Serum was collected from normal rats and from rats volume-expanded with isotonic sodium chloride solution. 2. The serum was fractionated by gel filtration on Sephadex G-25 and each fraction was tested for inhibitory activity against sodium—potassium-activated adenosine triphosphatase prepared from rat kidney homogenate. 3. A single low-molecular-weight fraction, eluting after the salts and after exogenously added lysine-vasopressin, had significantly greater enzyme inhibitory activity when obtained from serum of volume-expanded animals than from control serum. 4. As this fraction has been shown in previous independent studies to contain a natriuretic factor, it may be concluded that one property of this factor is the ability to inhibit sodium—potassium-activated adenosine triphosphatase.

The Morphology of the Rat Kidney Following Folic Acid Administration

1970 ◽  
Vol 28 ◽  
pp. 200-201
Author(s):  
Aline Byrnes ◽  
Elsa E. Ramos ◽  
Minoru Suzuki ◽  
E.D. Mayfield
Keyword(s):  
Folic Acid ◽  
De Novo ◽  
Specific Activity ◽  
Rat Kidney ◽  
Present Report ◽  
Intracellular Localization ◽  
Male Rats ◽  
Renal Hypertrophy ◽  
Electron Microscopic ◽  
Biochemical Alterations

Renal hypertrophy was induced in 100 g male rats by the injection of 250 mg folic acid (FA) dissolved in 0.3 M NaHCO3/kg body weight (i.v.). Preliminary studies of the biochemical alterations in ribonucleic acid (RNA) metabolism of the renal tissue have been reported recently (1). They are: RNA content and concentration, orotic acid-c14 incorporation into RNA and acid soluble nucleotide pool, intracellular localization of the newly synthesized RNA, and the specific activity of enzymes of the de novo pyrimidine biosynthesis pathway. The present report describes the light and electron microscopic observations in these animals. For light microscopy, kidney slices were fixed in formalin, embedded, sectioned, and stained with H & E and PAS.

Electron microscopic study of the membrane glycoproteins in the rat kidney after cisplatin treatment

1989 ◽  
Vol 47 ◽  
pp. 912-913
Author(s):  
S.K. Aggarwal
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Kidney ◽  
Light And Electron Microscopy ◽  
Kidney Tissue ◽  
Membrane Glycoproteins ◽  
Electron Microscopic ◽  
Before And After ◽  
Interstrand Cross Links ◽  
Cross Links

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

scholarly journals Sodium-Potassium-activated Adenosine Triphosphatase

1972 ◽  
Vol 247 (10) ◽  
pp. 3088-3092
Author(s):  
R. Wayne Albers ◽  
George J. Koval
Keyword(s):  
Adenosine Triphosphatase ◽  
Sodium Potassium
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