Neuropeptide Y System of the Female Monkey Hypothalamus: Retrograde Tracing and Immunostaining

Khushdev K. Thind; James E. Boggan; Paul C. Goldsmith

doi:10.1159/000126371

Efferent and Afferent Connections of Neuropeptide Y Neurons in the Nucleus Accumbens of Mice

Frontiers in Neuroanatomy ◽

10.3389/fnana.2021.741868 ◽

2021 ◽

Vol 15 ◽

Author(s):

Shunji Yamada ◽

Nienke van Kooten ◽

Takuma Mori ◽

Katsutoshi Taguchi ◽

Atsushi Tsujimura ◽

...

Keyword(s):

Nucleus Accumbens ◽

Neuropeptide Y ◽

Retrograde Tracing ◽

Cholera Toxin B Subunit ◽

Thalamic Nuclei ◽

Functional Roles ◽

Neural Connections ◽

Afferent Projections ◽

The Neural Networks ◽

Anterograde And Retrograde Tracing

Neuropeptide Y (NPY) is a neural peptide distributed widely in the brain and has various functions in each region. We previously reported that NPY neurons in the nucleus accumbens (NAc) are involved in the regulation of anxiety behavior. Anterograde and retrograde tracing studies suggest that neurons in the NAc project to several areas, such as the lateral hypothalamus (LH) and ventral pallidum (VP), and receive afferent projections from the cortex, thalamus, and amygdala. However, the neural connections between accumbal NPY neurons and other brain areas in mice remain unclear. In this study, we sought to clarify these anatomical connections of NPY neurons in the NAc by investigating their neural outputs and inputs. To selectively map NPY neuronal efferents from the NAc, we injected Cre-dependent adeno-associated viruses (AAVs) into the NAc of NPY-Cre mice. This revealed that NAc NPY neurons exclusively projected to the LH. We confirmed this by injecting cholera toxin b subunit (CTb), a retrograde tracer, into the LH and found that approximately 7–10% of NPY neurons in the NAc were double-labeled for mCherry and CTb. Moreover, retrograde tracing using recombinant rabies virus (rRABV) also identified NAc NPY projections to the LH. Finally, we investigated monosynaptic input to the NPY neurons in the NAc using rRABV. We found that NPY neurons in the NAc received direct synaptic connections from the midline thalamic nuclei and posterior basomedial amygdala. These findings provide new insight into the neural networks of accumbal NPY neurons and should assist in elucidating their functional roles.

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Nitric oxide synthase- and neuropeptide Y-containing subpopulations of sympathetic neurons in the coeliac ganglion of the Atlantic cod, Gadus morhua, revealed by immunohistochemistry and retrograde tracing from the stomach

Journal of the Autonomic Nervous System ◽

10.1016/s0165-1838(97)00042-8 ◽

1997 ◽

Vol 66 (1-2) ◽

pp. 35-45 ◽

Cited By ~ 21

Author(s):

Paul Karila ◽

Jenny Messenger ◽

Susanne Holmgren

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Neuropeptide Y ◽

Gadus Morhua ◽

Atlantic Cod ◽

Sympathetic Neurons ◽

Retrograde Tracing ◽

Oxide Synthase

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Synaptic inputs of neuropeptide Y-immunoreactive noradrenergic nerve terminals to neurons in the nucleus preopticus medianus which project to the paraventricular nucleus of the hypothalamus of the rat: a combined immunohistochemical and retrograde tracing method

Brain Research ◽

10.1016/0006-8993(93)90403-a ◽

1993 ◽

Vol 600 (1) ◽

pp. 74-80 ◽

Cited By ~ 27

Author(s):

Hitoshi Kawano ◽

Sadahiko Masuko

Keyword(s):

Neuropeptide Y ◽

Paraventricular Nucleus ◽

Retrograde Tracing ◽

Nerve Terminals ◽

Synaptic Inputs ◽

Tracing Method

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Identification of neuropeptide Y in superior cervical ganglion neurons that project to the oesophagus – A combined immunohistochemical labelling and retrograde tracing study in pigs

Acta Veterinaria Hungarica ◽

10.1556/004.2019.011 ◽

2019 ◽

Vol 67 (1) ◽

pp. 98-105 ◽

Cited By ~ 1

Author(s):

Liliana Rytel ◽

Anna Snarska ◽

Slawomir Gonkowski ◽

Joanna Wojtkiewicz ◽

Ottó Szenci ◽

...

Keyword(s):

Neuropeptide Y ◽

Superior Cervical Ganglion ◽

Active Substance ◽

Retrograde Tracing ◽

Gi Tract ◽

Neuronal Tracing ◽

Cervical Oesophagus ◽

Cervical Ganglion ◽

Extrinsic Innervation ◽

Ganglion Neurons

Neuropeptide Y (NPY) is a neuronal active substance taking part in the regulation of gastrointestinal (GI) tract activity. This study used retrograde neuronal tracing and immunofluorescence methods to analyse NPY-positive neurons located in superior cervical ganglion and supplying the cervical oesophagus in the pig. The presence of NPY was observed in 30% of all neurons supplying the part of oesophagus studied. Probably the number of Fast Blue (FB) positive cells depends on the area of the wall injected with FB and the fragment of oesophagus studied. Therefore, the obtained results indicate that the described peptide is an important factor in the extrinsic innervation of this part of the GI tract.

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