A Score Method for Comparison of Partial Genomic Regions in Their Representatives of Full-Length Genome of Hepatitis E Virus for Genotyping

Intervirology ◽  
2007 ◽  
Vol 50 (5) ◽  
pp. 328-335 ◽  
Author(s):  
Pengcheng Xun ◽  
Feng Chen ◽  
Chen Dong ◽  
Guohua Qian ◽  
Dejian Lai ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Score Method ◽  
Genomic Regions ◽  
Full Length Genome

Full-length genome of a novel genotype 3 hepatitis E virus strain obtained from domestic pigs in Japan

2017 ◽  
Vol 240 ◽  
pp. 147-153 ◽  
Author(s):  
Putu Prathiwi Primadharsini ◽  
Masao Miyake ◽  
Satoshi Kunita ◽  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
...  
Keyword(s):  
Virus Strain ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Genotype 3 ◽  
Domestic Pigs ◽  
Novel Genotype ◽  
Full Length Genome

Analysis of the full-length genome of genotype 4 hepatitis E virus isolates from patients with fulminant or acute self-limited hepatitis E

2006 ◽  
Vol 78 (4) ◽  
pp. 476-484 ◽  
Author(s):  
Jun Inoue ◽  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
Tatsuya Aikawa ◽  
Hitoshi Mizuo ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Genotype 4 ◽  
Full Length Genome ◽  
Virus Isolates

scholarly journals Analysis of the full-length genome of hepatitis E virus isolates obtained from wild boars in Japan

2005 ◽  
Vol 86 (12) ◽  
pp. 3321-3326 ◽  
Author(s):  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
Kazunori Endo ◽  
Shinji Fujiwara ◽  
Nobuo Sakuma ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Partial Sequence ◽  
Genotype 3 ◽  
Entire Genome ◽  
Wild Boars ◽  
Full Length Sequence ◽  
Full Length Genome ◽  
Virus Isolates

Two (2·3 %) of 87 wild-caught boars in Japan had detectable hepatitis E virus (HEV) RNA. The two boar HEV isolates (wbJTS1 and wbJYG1) obtained in the present study and a previously reported isolate (wbJSG1) whose partial sequence had been determined were sequenced over the entire genome. The wbJSG1, wbJTS1 and wbJYG1 isolates comprised 7225 or 7226 nt, excluding the poly(A) tail, and segregated into genotype 3. They differed by 8·5–11·2 % from each other and by 8·6–18·4 % from 17 reported genotype 3 HEV isolates, including one boar isolate, in the full-length sequence. When compared with 191 reported genotype 3 HEV isolates whose partial sequences were known, these three boar isolates were closer to Japanese isolates than to isolates of non-Japanese origin (89·2±2·6 vs 85·9±2·2 %; P<0·0001). A proportion of wild boars in Japan are infected with markedly heterogeneous HEV strains that are indigenous to Japan and may serve as reservoirs of HEV.

Analysis of the full-length genome of hepatitis E virus isolates obtained from farm pigs in Mongolia

2007 ◽  
Vol 79 (8) ◽  
pp. 1128-1137 ◽  
Author(s):  
Felipe R. Lorenzo ◽  
Bira Tsatsralt-Od ◽  
Sanjaa Ganbat ◽  
Masaharu Takahashi ◽  
Hiroaki Okamoto
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Full Length Genome ◽  
Virus Isolates

Marked genomic heterogeneity of rat hepatitis E virus strains in Indonesia demonstrated on a full-length genome analysis

2014 ◽  
Vol 179 ◽  
pp. 102-112 ◽  
Author(s):  
Mulyanto ◽  
Joseph Benedictus Suparyatmo ◽  
I Gusti Ayu Sri Andayani ◽  
Khalid ◽  
Masaharu Takahashi ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Full Length Genome ◽  
Virus Strains

scholarly journals Comprehensive Molecular Approach for Characterization of Hepatitis E Virus Genotype 3 Variants

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Bo Wang ◽  
Dominik Harms ◽  
C. Patrick Papp ◽  
Sandra Niendorf ◽  
Sonja Jacobsen ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis E Virus ◽  
Phylogenetic Analyses ◽  
Hepatitis E ◽  
Full Length ◽  
Molecular Approach ◽  
Genotype 3 ◽  
Genome Sequences ◽  
Virus Genotype ◽  
Pcr Targeting

ABSTRACT Autochthonous hepatitis E virus genotype 3 (HEV-3) infections in industrialized countries are more frequent than previously assumed. HEV-3 is zoonotic and the causal pathogen of chronic hepatitis E. According to the latest classification of the family Hepeviridae , 10 designated HEV-3 subtypes (HEV-3a to HEV-3j) and 7 unassigned HEV-3 subtypes are proposed. In order to identify and characterize the HEV-3 variants in circulation, we developed a molecular approach combining a sensitive HEV-specific real-time reverse transcription-PCR (RT-PCR) targeting the overlapping region of HEV ORF2 and ORF3 (the ORF2/3 region) and two newly designed consensus nested RT-PCRs targeting the HEV ORF1 and ORF2 genes, respectively. Since complete genome sequences are required for new HEV-3 subtype assignment, we implemented a straightforward approach for full-length HEV-3 genome amplification. Twenty-nine human serum samples and six human feces samples from chronic hepatitis E patients were selected for evaluation of the system. Viral loads ranged from 1 × 10 4 to 1.9 × 10 10 copies/ml of serum and from 1.8 × 10 4 to 1 × 10 12 copies/g of feces. Sequence and phylogenetic analyses of partial ORF1 and ORF2 sequences showed that HEV strains had considerable genetic diversity and clustered into the HEV-3c (29/35), HEV-3e (2/35), HEV-3f (2/35), and unassigned HEV-3 (2/35) subtypes. Moreover, from these strains, three full-length HEV-3 genome sequences were generated and characterized. DE/15-0030 represents a typical HEV-3c strain (95.7% nucleotide identity to wbGER27), while DE/15-0031 and SW/16-0282 have <89.2% homology to known HEV-3 strains and are phylogenetically divergent, indicating novel HEV-3 subtypes. In summary, our approach will significantly facilitate the detection, quantification, and determination of HEV-3 strains and will thus help to improve molecular diagnostics and our knowledge of HEV diversity and evolution.

scholarly journals RNA transcripts of full-length cDNA clones of rabbit hepatitis E virus are infectious in rabbits

2015 ◽  
Vol 96 (5) ◽  
pp. 1190-1190
Author(s):  
Caitlin M. Cossaboom ◽  
Yao-Wei Huang ◽  
Danielle M. Yugo ◽  
Scott P. Kenney ◽  
Pablo Piñeyro ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna ◽  
Rna Transcripts
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