Dynamics of Glucocorticoid Receptor and Mineralocorticoid Receptor: Implications from Live Cell Imaging Studies

2007 ◽  
Vol 85 (3) ◽  
pp. 186-192 ◽  
Author(s):  
Mayumi Nishi ◽  
Mitsuhiro Kawata
Keyword(s):  
Glucocorticoid Receptor ◽  
Mineralocorticoid Receptor ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Studies

scholarly journals Direct observation of aggregate-triggered selective autophagy

2021 ◽  
Author(s):  
Anne FJ Janssen ◽  
Giel Korsten ◽  
Wilco Nijenhuis ◽  
Eugene Katrukha ◽  
Lukas Kapitein
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Relative Timing ◽  
Imaging Studies ◽  
Selective Autophagy ◽  
The Core ◽  
Spatiotemporal Regulation ◽  
Core Components ◽  
Inducible Protein

Degradation of aggregates by selective autophagy is important as damaged proteins may impose a threat to cellular homeostasis. Although the core components of the autophagy machinery are well-characterized, the spatiotemporal regulation of many selective autophagy processes, including aggrephagy, remains largely unexplored. Furthermore, because most live-cell imaging studies have so far focused on starvation-induced autophagy, little is known about the dynamics of aggrephagy. Here, we describe the development and application of the mKeima-PIM assay, which enables live-cell observation of autophagic turnover and degradation of inducible protein aggregates in conjunction with key autophagy players. This allowed us to quantify the relative timing and duration of different steps of aggrephagy and revealed the short-lived nature of the autophagosome. The assay furthermore showed the spatial distribution of omegasome formation, highlighting that autophagy initiation is directly instructed by the cargo. Moreover, we found that nascent autophagosomes mostly remain immobile until acidification occurs. Thus, our assay provides new insights into the spatiotemporal regulation and dynamics of aggrephagy.

scholarly journals A chemosensor for Al3+ ions in aqueous ethanol media: photophysical and live cell imaging studies

RSC Advances ◽  
2016 ◽  
Vol 6 (22) ◽  
pp. 17995-18001 ◽  
Author(s):  
Nabanita Chatterjee ◽  
Shubhra Bikash Maity ◽  
Asmita Samadder ◽  
Puspal Mukherjee ◽  
Anisur Rahman Khuda-Bukhsh ◽  
...  
Keyword(s):  
Metal Ions ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous Ethanol ◽  
Live Cell ◽  
Fluorescent Chemosensor ◽  
Selective Detection ◽  
Imaging Studies ◽  
Turn On ◽  
Highly Sensitive

A new rhodamine based highly sensitive “Turn-ON” fluorescent chemosensor L for the selective detection of Al3+ ion over other biologically competing metal ions in aqueous ethanol media.

scholarly journals Live Cell Imaging Unveils Multiple Domain Requirements for In Vivo Dimerization of the Glucocorticoid Receptor

PLoS Biology ◽  
2014 ◽  
Vol 12 (3) ◽  
pp. e1001813 ◽  
Author(s):  
Diego M. Presman ◽  
M. Florencia Ogara ◽  
Martín Stortz ◽  
Lautaro D. Alvarez ◽  
John R. Pooley ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Multiple Domain

scholarly journals Assembly of multiprotein complexes that control genome function

2009 ◽  
Vol 185 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Christoffel Dinant ◽  
Martijn S. Luijsterburg ◽  
Thomas Höfer ◽  
Gesa von Bornstaedt ◽  
Wim Vermeulen ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Dna Repair ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Studies ◽  
Multiprotein Complexes ◽  
Insight Into

Live-cell imaging studies aided by mathematical modeling have provided unprecedented insight into assembly mechanisms of multiprotein complexes that control genome function. Such studies have unveiled emerging properties of chromatin-associated systems involved in DNA repair and transcription.

scholarly journals pH-Sensitive Pegylated Cationic Lipid-DNA Complexes for Gene Delivery: Transfection Efficiency and Live Cell Imaging Studies

2012 ◽  
Vol 102 (3) ◽  
pp. 501a-502a ◽  
Author(s):  
Chia-Ling Chan ◽  
Ramsey Majzoub ◽  
Rahau S. Shirazi ◽  
Keng-San Liang ◽  
Kai K. Ewert ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Transfection Efficiency ◽  
Cationic Lipid ◽  
Ph Sensitive ◽  
Live Cell ◽  
Imaging Studies ◽  
Dna Complexes
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