Stabilization of G-Quadruplex DNA, Inhibition of Telomerase Activity and Live Cell Imaging Studies of Chiral Ruthenium(II) Complexes

2012 ◽  
Vol 18 (14) ◽  
pp. 4285-4295 ◽  
Author(s):  
Dongdong Sun ◽  
Yanan Liu ◽  
Du Liu ◽  
Rong Zhang ◽  
Xicheng Yang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Telomerase Activity ◽  
Live Cell ◽  
Imaging Studies ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Dna Inhibition

scholarly journals Multivalent Self-Assembled DNA Polymer for Tumor-Targeted Delivery and Live Cell Imaging of Telomerase Activity

2018 ◽  
Vol 90 (22) ◽  
pp. 13188-13192 ◽  
Author(s):  
Xueli Zhu ◽  
Hongyan Ye ◽  
Jin-Wen Liu ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Targeted Delivery ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Telomerase Activity ◽  
Live Cell ◽  
Self Assembled

scholarly journals Direct observation of aggregate-triggered selective autophagy

2021 ◽  
Author(s):  
Anne FJ Janssen ◽  
Giel Korsten ◽  
Wilco Nijenhuis ◽  
Eugene Katrukha ◽  
Lukas Kapitein
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Relative Timing ◽  
Imaging Studies ◽  
Selective Autophagy ◽  
The Core ◽  
Spatiotemporal Regulation ◽  
Core Components ◽  
Inducible Protein

Degradation of aggregates by selective autophagy is important as damaged proteins may impose a threat to cellular homeostasis. Although the core components of the autophagy machinery are well-characterized, the spatiotemporal regulation of many selective autophagy processes, including aggrephagy, remains largely unexplored. Furthermore, because most live-cell imaging studies have so far focused on starvation-induced autophagy, little is known about the dynamics of aggrephagy. Here, we describe the development and application of the mKeima-PIM assay, which enables live-cell observation of autophagic turnover and degradation of inducible protein aggregates in conjunction with key autophagy players. This allowed us to quantify the relative timing and duration of different steps of aggrephagy and revealed the short-lived nature of the autophagosome. The assay furthermore showed the spatial distribution of omegasome formation, highlighting that autophagy initiation is directly instructed by the cargo. Moreover, we found that nascent autophagosomes mostly remain immobile until acidification occurs. Thus, our assay provides new insights into the spatiotemporal regulation and dynamics of aggrephagy.

Stabilization of G-Quadruplex DNA, Inhibition of Telomerase Activity, and Tumor Cell Apoptosis by Organoplatinum(II) Complexes with Oxoisoaporphine

2015 ◽  
Vol 58 (5) ◽  
pp. 2159-2179 ◽  
Author(s):  
Zhen-Feng Chen ◽  
Qi-Pin Qin ◽  
Jiao-Lan Qin ◽  
Yan-Cheng Liu ◽  
Ke-Bin Huang ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Apoptosis ◽  
Telomerase Activity ◽  
Tumor Cell Apoptosis ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Dna Inhibition

scholarly journals A chemosensor for Al3+ ions in aqueous ethanol media: photophysical and live cell imaging studies

RSC Advances ◽  
2016 ◽  
Vol 6 (22) ◽  
pp. 17995-18001 ◽  
Author(s):  
Nabanita Chatterjee ◽  
Shubhra Bikash Maity ◽  
Asmita Samadder ◽  
Puspal Mukherjee ◽  
Anisur Rahman Khuda-Bukhsh ◽  
...  
Keyword(s):  
Metal Ions ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous Ethanol ◽  
Live Cell ◽  
Fluorescent Chemosensor ◽  
Selective Detection ◽  
Imaging Studies ◽  
Turn On ◽  
Highly Sensitive

A new rhodamine based highly sensitive “Turn-ON” fluorescent chemosensor L for the selective detection of Al3+ ion over other biologically competing metal ions in aqueous ethanol media.

A small-sized benzothiazole–indolium fluorescent probe: the study of interaction specificity targeting c-MYC promoter G-quadruplex structures and live cell imaging

2020 ◽  
Vol 56 (95) ◽  
pp. 15016-15019
Author(s):  
Bo-Xin Zheng ◽  
Meng-Ting She ◽  
Wei Long ◽  
Yong-Yu Xu ◽  
Yi-Han Zhang ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
G Quadruplex ◽  
In Cells

A small-sized and target-specific fluorescent probe reveals the presence of c-MYC DNA G4-structures in cells and shows anticancer activity.

scholarly journals Assembly of multiprotein complexes that control genome function

2009 ◽  
Vol 185 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Christoffel Dinant ◽  
Martijn S. Luijsterburg ◽  
Thomas Höfer ◽  
Gesa von Bornstaedt ◽  
Wim Vermeulen ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Dna Repair ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Studies ◽  
Multiprotein Complexes ◽  
Insight Into

Live-cell imaging studies aided by mathematical modeling have provided unprecedented insight into assembly mechanisms of multiprotein complexes that control genome function. Such studies have unveiled emerging properties of chromatin-associated systems involved in DNA repair and transcription.

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