scholarly journals A Suggestive Association of Fuchs Heterochromic Cyclitis with Cytotoxic T Cell Antigen 4 Gene Polymorphism

2007 ◽  
Vol 39 (2) ◽  
pp. 116-120 ◽  
Author(s):  
Bernd M. Spriewald ◽  
Carolina Lefter ◽  
Ilka Huber ◽  
Birgit Lauer ◽  
Hartmut Wenkel
Keyword(s):  
T Cell ◽  
Gene Polymorphism ◽  
Cytotoxic T Cell ◽  
Cell Antigen ◽  
Suggestive Association ◽  
Fuchs Heterochromic Cyclitis

scholarly journals Fluorescent target array killing assay: A multiplex cytotoxic T-cell assay to measure detailed T-cell antigen specificity and avidity in vivo

2012 ◽  
Vol 81A (8) ◽  
pp. 679-690 ◽  
Author(s):  
Benjamin J. C. Quah ◽  
Danushka K. Wijesundara ◽  
Charani Ranasinghe ◽  
Christopher R. Parish
Keyword(s):  
T Cell ◽  
Cytotoxic T Cell ◽  
Antigen Specificity ◽  
Killing Assay ◽  
Cell Antigen ◽  
Cell Assay ◽  
Target Array

scholarly journals Immunophenotype of T Cells Expressing Programmed Death-1 and Cytotoxic T Cell Antigen-4 in Early Lung Cancer: Local vs. Systemic Immune Response

Cancers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 567 ◽  
Author(s):  
Iwona Kwiecien ◽  
Tomasz Skirecki ◽  
Małgorzata Polubiec-Kownacka ◽  
Agata Raniszewska ◽  
Joanna Domagala-Kulawik
Keyword(s):  
Lung Cancer ◽  
T Cells ◽  
T Cell ◽  
Local Environment ◽  
Lavage Fluid ◽  
Cytotoxic T Cell ◽  
Multiparameter Flow Cytometry ◽  
Cell Antigen ◽  
Programmed Death ◽  
Programmed Death 1

The overexpression of programmed death-1 (PD-1) and cytotoxic T cell antigen 4 (CTLA-4) receptors on T cells are among the major mechanisms of tumor immunoevasion. However, the expression pattern of these receptors on T cell subpopulations of a different activation status and at different sites is poorly characterized. Thus, we analyzed the expression of PD-1 and CTLA-4 on the naïve, activated, memory, and activated memory T cells. Bronchoalveolar lavage fluid (BALF) from the lung affected by lung cancer (clBALF), the opposite ‘healthy’ lung (hlBALF), and peripheral blood (PB) samples were collected from 32 patients. The cells were analyzed by multiparameter flow cytometry. The proportion of memory, activated, and activated memory CD8+ cells with the expression of PD-1 and CTLA-4 were elevated in the clBALF when compared to the hlBALF (insignificantly), but these proportions were significantly higher in the BALF when compared with the PB. The proportions of PD-1+ and CTLA-4+ T cells were elevated in the squamous cell carcinoma when compared to the adenocarcinoma patients. Also, the expression of PD-1 and CTLA-4 on T cells from the BALF was significantly higher than from PB. We report for the first time the differential expression of checkpoint molecules on CD4+ and CD8+ lymphocytes at a different stage of activation in the local environment of lung cancer. Moreover, the circulating T cells have a distinct expression of these receptors, which suggests their poor utility as biomarkers for immunotherapy.

Cytotoxic T cell antigen 4 and the status of T cell activation in lung cancer: local vs. systemic immune response

2017 ◽  
Author(s):  
Iwona Kwiecień ◽  
Tomasz Skirecki ◽  
Małgorzata Polubiec-Kownacka ◽  
Dariusz Dziedzic ◽  
Joanna Domagała-Kulawik
Keyword(s):  
Lung Cancer ◽  
Immune Response ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Cytotoxic T Cell ◽  
Systemic Immune Response ◽  
Cell Antigen ◽  
The Status

scholarly journals Phenotypic characterization of skin-infiltrating T cells in cutaneous T- cell lymphoma: comparison with benign cutaneous T-cell infiltrates

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 463-473 ◽  
Author(s):  
BF Haynes ◽  
LL Hensley ◽  
BV Jegasothy
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Skin Tests ◽  
Cytotoxic T Cell ◽  
Phenotypic Characterization ◽  
Cell Phenotype ◽  
Cutaneous T Cell Lymphoma ◽  
Cell Antigen

Abstract Using a panel of monoclonal antibodies, we have studied cell surface antigens of infiltrating mononuclear cells in skin biopsies from patients with cutaneous T-cell lymphoma (CTCL) and compared them with the T-cell surface phenotype seen in benign cutaneous T-cell infiltrations (e.g., contact dermatitis, delayed hypersensitivity skin tests, granuloma annulare) and in dermal infiltrates of lymphomatoid granulomatosis patients. We found that unlike circulating CTCL (Sezary) cells, CTCL cells infiltrating skin epidermis frequently expressed the T-cell antigen 3A1. Cutaneous infiltrates in 10 patients with mycosis fungoides (MF) and 1 patient with Sezary syndrome were OKT4 (inducer T cell), OKT8 (suppressor/cytotoxic T cell); 2 patients with MF were OKT4- , OKT8; and one MF patients's skin T cell were OKT4-, OKT8+. Similar to CTCL infiltrating cells, most of the benign skin T-cell infiltrates were usually 3A1+. OKT4+, and OKT8-. Our study shows the complex nature of T-cell antigen patterns in inflammatory and malignant skin T-cell infiltrations. We demonstrated that the CTCL the skin epidermal infiltrating T-cell phenotype is not invariate, and in many cases, is similar to the phenotype of clinically benign cutaneous T-cell infiltrations.

scholarly journals Phenotypic characterization of skin-infiltrating T cells in cutaneous T- cell lymphoma: comparison with benign cutaneous T-cell infiltrates

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 463-473
Author(s):  
BF Haynes ◽  
LL Hensley ◽  
BV Jegasothy
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Skin Tests ◽  
Cytotoxic T Cell ◽  
Phenotypic Characterization ◽  
Cell Phenotype ◽  
Cutaneous T Cell Lymphoma ◽  
Cell Antigen

Using a panel of monoclonal antibodies, we have studied cell surface antigens of infiltrating mononuclear cells in skin biopsies from patients with cutaneous T-cell lymphoma (CTCL) and compared them with the T-cell surface phenotype seen in benign cutaneous T-cell infiltrations (e.g., contact dermatitis, delayed hypersensitivity skin tests, granuloma annulare) and in dermal infiltrates of lymphomatoid granulomatosis patients. We found that unlike circulating CTCL (Sezary) cells, CTCL cells infiltrating skin epidermis frequently expressed the T-cell antigen 3A1. Cutaneous infiltrates in 10 patients with mycosis fungoides (MF) and 1 patient with Sezary syndrome were OKT4 (inducer T cell), OKT8 (suppressor/cytotoxic T cell); 2 patients with MF were OKT4- , OKT8; and one MF patients's skin T cell were OKT4-, OKT8+. Similar to CTCL infiltrating cells, most of the benign skin T-cell infiltrates were usually 3A1+. OKT4+, and OKT8-. Our study shows the complex nature of T-cell antigen patterns in inflammatory and malignant skin T-cell infiltrations. We demonstrated that the CTCL the skin epidermal infiltrating T-cell phenotype is not invariate, and in many cases, is similar to the phenotype of clinically benign cutaneous T-cell infiltrations.

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