Differentiation of Follicular Thyroid Adenoma from Carcinoma by Means of Gene Expression Profiling with Adapter-Tagged Competitive Polymerase Chain Reaction

Oncology ◽  
2005 ◽  
Vol 69 (5) ◽  
pp. 428-435 ◽  
Author(s):  
Kazuya Taniguchi ◽  
Toru Takano ◽  
Akira Miyauchi ◽  
Kyoko Koizumi ◽  
Yasuhiro Ito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Thyroid Adenoma ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Follicular Thyroid Adenoma ◽  
Polymerase Chain

Real-time polymerase chain reaction-based exponential sample amplification for microarray gene expression profiling

2005 ◽  
Vol 337 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Zsolt B. Nagy ◽  
János Z. Kelemen ◽  
Liliána Z. Fehér ◽  
Ágnes Zvara ◽  
Kata Juhász ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Gene Expression Profiling ◽  
Real Time ◽  
Expression Profiling ◽  
Chain Reaction ◽  
Microarray Gene Expression ◽  
Polymerase Chain ◽  
Microarray Gene

scholarly journals Myelin-forming cell-specific cadherin-19 is a marker for minimally infiltrative glioblastoma stem-like cells

2015 ◽  
Vol 122 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Michael Zorniak ◽  
Paul A. Clark ◽  
John S. Kuo
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Plasma Membrane ◽  
Glioblastoma Multiforme ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Quantitative Polymerase Chain Reaction ◽  
Normal Brain ◽  
Chain Reaction ◽  
Polymerase Chain

OBJECT Glioblastoma stem-like cells (GSCs) exhibit stem-like properties, are highly efficient at forming tumor xenografts, and are resistant to many current therapies. Current molecular identifiers of GSCs are scarce and controversial. The authors describe differential cell-surface gene expression profiling to identify GSC-specific markers. METHODS Independent human GSC lines were isolated and maintained in standard neural stem cell (NSC) media and were validated for self-renewal, multipotent differentiation, and tumor initiation properties. Candidate upregulated GSCspecific plasma membrane markers were identified through differential Affymetrix U133 Plus 2.0 Array gene expression profiling of GSCs, human NSCs (hNSCs), normal brain tissue, and primary/recurrent glioblastoma multiforme samples. Results were validated by using comparative quantitative reverse transcription polymerase chain reaction and Western blot analysis of GSCs, hNSCs, normal human astrocytes, U87 glioma cell line, and patient-matched serum-cultured glioblastoma multiforme samples. RESULTS A candidate GSC-specific signature of 19 upregulated known and novel plasma membrane–associated genes was identified. Preferential upregulation of these plasma membrane–linked genes was validated by quantitative polymerase chain reaction. Cadherin-19 (CDH19) protein expression was enhanced in minimally infiltrative GSC lines. CONCLUSIONS Gene expression profiling of GSCs has shown CDH19 to be an exciting new target for drug development and study of GBM tumorigenesis.

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230-240 ◽  
Author(s):  
Marianne Jörgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

Measurement of Gene Expression by Multiplex Competitive Polymerase Chain Reaction

1993 ◽  
Vol 213 (2) ◽  
pp. 277-284 ◽  
Author(s):  
M.J. Apostolakos ◽  
W.H.T. Schuermann ◽  
M.W. Frampton ◽  
M.J. Utell ◽  
J.C. Willey
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain
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