In situ Characterization of Gonadotropin- Releasing Hormone-I, -III, and Glutamic Acid Decarboxylase Expression in the Brain of the Sea Lamprey, Petromyzon marinus

2004 ◽  
Vol 65 (1) ◽  
pp. 60-70 ◽  
Author(s):  
Adam R. Root ◽  
Nathaniel V. Nucci ◽  
Jocelyn D. Sanford ◽  
Beverly S. Rubin ◽  
Vance L. Trudeau ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Gonadotropin Releasing Hormone ◽  
Acid Decarboxylase ◽  
In Situ Characterization ◽  
The Brain

scholarly journals In situ characterization of the brain–microdevice interface using Device Capture Histology

2011 ◽  
Vol 201 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Andrew J. Woolley ◽  
Himanshi A. Desai ◽  
Mitchell A. Steckbeck ◽  
Neil K. Patel ◽  
Kevin J. Otto
Keyword(s):  
In Situ Characterization ◽  
The Brain

Synthesis and Relaxometric Characterization of a MRI Gd-Based Probe Responsive to Glutamic Acid Decarboxylase Enzymatic Activity

2013 ◽  
Vol 56 (6) ◽  
pp. 2466-2477 ◽  
Author(s):  
Roberta Napolitano ◽  
Giorgio Pariani ◽  
Franco Fedeli ◽  
Zsolt Baranyai ◽  
Markus Aswendt ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase

scholarly journals Disruption of Hippocampal Neuregulin 1–ErbB4 Signaling Contributes to the Hippocampus-dependent Cognitive Impairment Induced by Isoflurane in Aged Mice

2014 ◽  
Vol 121 (1) ◽  
pp. 79-88 ◽  
Author(s):  
Xiao-Min Li ◽  
Fan Su ◽  
Mu-Huo Ji ◽  
Guang-Fen Zhang ◽  
Li-Li Qiu ◽  
...  
Keyword(s):  
Cognitive Impairment ◽  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acid Decarboxylase ◽  
Neuregulin 1 ◽  
Aged Mice ◽  
Vehicle Injection ◽  
To Receive ◽  
The Brain

Abstract Background: A prolonged isoflurane exposure may lead to cognitive decline in rodents. Neuregulin 1 (NRG1)–ErbB4 signaling plays a key role in the modulation of hippocampal synaptic plasticity through regulating the neurotransmission. The authors hypothesized that hippocampal NRG1–ErbB4 signaling is involved in isoflurane-induced cognitive impairments in aged mice. Methods: Fourteen-month-old C57BL/6 mice were randomized to receive 100% O2 exposure, vehicle injection after 100% O2 exposure, vehicle injection after exposure to isoflurane carried by 100% O2, NRG1-β1 injection after exposure to isoflurane carried by 100% O2, and NRG1-β1 and an ErbB4 inhibitor AG1478 injection after exposure to isoflurane carried by 100% O2. Fear conditioning test was used to assess the cognitive function of mice 48-h postexposure. The brain tissues were harvested 48-h postexposure to determine the levels of NRG1, ErbB4, p-ErbB4, parvalbumin, and glutamic acid decarboxylase 67 in the hippocampus using Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence. Results: The percentage of freezing time to context was decreased from 50.28 ± 11.53% to 30.82 ± 10.00%, and the hippocampal levels of NRG1, p-ErbB4/ErbB4, parvalbumin, and glutamic acid decarboxylase 67 were decreased from 172.79 ± 20.85 ng/g, 69.15 ± 12.20%, 101.68 ± 11.21%, and 104.71 ± 6.85% to 112.92 ± 16.65 ng/g, 42.26 ± 9.71%, 75.89 ± 10.26%, and 73.87 ± 16.89%, respectively, after isoflurane exposure. NRG1-β1 attenuated the isoflurane-induced hippocampus-dependent cognitive impairment and the declines in the hippocampal NRG1, p-ErbB4/ErbB4, parvalbumin, and glutamic acid decarboxylase 67. AG1478 inhibited the rescuing effects of NRG1-β1. Conclusion: Disruption of NRG1–ErbB4 signaling in the parvalbumin-positive interneurons might, at least partially, contribute to the isoflurane-induced hippocampus-dependent cognitive impairment after exposure to isoflurane carried by 100% O2 in aged mice.

Simultaneous detection of glutamic acid decarboxylase and reelin mRNA in adult rat neurons using in situ hybridization and immunofluorescence

1998 ◽  
Vol 3 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Christine Pesold ◽  
Maria G Pisu ◽  
Francesco Impagnatiello ◽  
Doncho P Uzunov ◽  
Hector J Caruncho
Keyword(s):  
In Situ Hybridization ◽  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Simultaneous Detection ◽  
Acid Decarboxylase ◽  
Adult Rat
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