scholarly journals Characterization of patients with diabetes who were incidentally found to be glutamic acid decarboxylase autoantibody‐positive by bridging‐type enzyme‐linked immunosorbent assay

2020 ◽  
Vol 11 (6) ◽  
pp. 1507-1510
Author(s):  
Eiji Kawasaki ◽  
Takahiro Fukuyama ◽  
Aira Uchida ◽  
Yoko Sagara ◽  
Hidekazu Tamai ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Immunosorbent Assay ◽  
Glutamic Acid Decarboxylase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acid Decarboxylase ◽  
Glutamic Acid Decarboxylase Autoantibody ◽  
Patients With Diabetes

Japanese cases of acute onset diabetic ketosis without acidosis in the absence of glutamic acid decarboxylase autoantibody

Endocrine ◽  
2010 ◽  
Vol 37 (2) ◽  
pp. 286-288 ◽  
Author(s):  
Yorihiro Iwasaki ◽  
Yoshiyuki Hamamoto ◽  
Yukiko Kawasaki ◽  
Hiroki Ikeda ◽  
Sachiko Honjo ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Acute Onset ◽  
Acid Decarboxylase ◽  
Diabetic Ketosis ◽  
Glutamic Acid Decarboxylase Autoantibody

scholarly journals Comparison of Measurements of Autoantibodies to Glutamic Acid Decarboxylase and Islet Antigen-2 in Whole Blood Eluates from Dried Blood Spots Using the RSR-Enzyme Linked Immunosorbent Assay Kits and In-House Radioimmunoassays

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Anders Persson ◽  
Charlotte Becker ◽  
Ida Hansson ◽  
Anita Nilsson ◽  
Carina Törn
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Dried Blood Spots ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acid Decarboxylase ◽  
Blood Spots ◽  
And Control ◽  
Islet Antigen ◽  
Prozone Effect

To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%;P=.008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%;P<.001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.

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