Experimental Leishmania major Infection Suppresses HIV-1 DNA Vaccine Induced Cellular Immune Response

2004 ◽  
Vol 177 (3) ◽  
pp. 185-188 ◽  
Author(s):  
Tara M. Robinson ◽  
Robin Nelson ◽  
David Artis ◽  
Phillip Scott ◽  
Jean D. Boyer
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Leishmania Major ◽  
Cellular Immune ◽  
Hiv 1

Attenuated nef DNA vaccine construct induces cellular immune response: role in HIV-1 multiprotein vaccine

2003 ◽  
Vol 89 (2-3) ◽  
pp. 207-214 ◽  
Author(s):  
Biswanath Majumder ◽  
Benjamin Gray ◽  
Sean McBurney ◽  
Todd M. Schaefer ◽  
Tzvete Dentchev ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Cellular Immune ◽  
Hiv 1

Enhancement of Cellular Immune Response in HIV-1 Seropositive Individuals: A DNA-Based Trial

1999 ◽  
Vol 90 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Jean D. Boyer ◽  
Michael A. Chattergoon ◽  
Kenneth E. Ugen ◽  
Ami Shah ◽  
Mosi Bennett ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Cellular Immune ◽  
Hiv 1

Improved Cellular Immune Response Elicited by a Ubiquitin-Fused DNA Vaccine AgainstMycobacterium tuberculosis

2012 ◽  
Vol 31 (4) ◽  
pp. 489-495 ◽  
Author(s):  
Qingmin Wang ◽  
Chengxiang Lei ◽  
Hui Wan ◽  
Qiuhong Liu
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Cellular Immune

Success and failure of the cellular immune response against HIV-1

2015 ◽  
Vol 16 (6) ◽  
pp. 563-570 ◽  
Author(s):  
Stephen A Migueles ◽  
Mark Connors
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Cellular Immune ◽  
Hiv 1

scholarly journals Induction of Cellular Immune Response by DNA Vaccine CoexpressingE. acervulina3-1E Gene and Mature CHIl-15 Gene

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Dexing Ma ◽  
Chunli Ma ◽  
Mingyang Gao ◽  
Guangxing Li ◽  
Ze Niu ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Lymphocyte Proliferation ◽  
Vaccine Development ◽  
Flow Cytometric Analysis ◽  
Potential Candidate ◽  
Spleen Lymphocyte ◽  
Cellular Immune ◽  
And Control

We previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linkingEimeria acervulina3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge. In the present study, the induction of cellular immune response induced by the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15 was investigated. Spleen lymphocyte subpopulations were characterized by flow cytometric analysis. The spleen lymphocyte proliferation assays were measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide (MTT) method. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. Chickens immunized with pcDNA-3-1E-linker-mChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen following two immunizations compared with chickens in other groups (P<0.01). In comparison with pcDNA3.1-immunized and control groups, lymphocyte proliferation, percentage of CD8α+cell, and levels of ChIL-2 and ChIFN-γ transcripts in the group immunized with pcDNA-3-1E-linker-mChIL-15 were significantly increased on day 6 following challenge (P<0.05,P<0.01, andP<0.01, resp.). Our data suggested that the fusion antigen 3-1E-linker-mChIL-15 could be a potential candidate forE. acervulinavaccine development.

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