scholarly journals Induction of Cellular Immune Response by DNA Vaccine CoexpressingE. acervulina3-1E Gene and Mature CHIl-15 Gene

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Dexing Ma ◽  
Chunli Ma ◽  
Mingyang Gao ◽  
Guangxing Li ◽  
Ze Niu ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Lymphocyte Proliferation ◽  
Vaccine Development ◽  
Flow Cytometric Analysis ◽  
Potential Candidate ◽  
Spleen Lymphocyte ◽  
Cellular Immune ◽  
And Control

We previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linkingEimeria acervulina3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge. In the present study, the induction of cellular immune response induced by the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15 was investigated. Spleen lymphocyte subpopulations were characterized by flow cytometric analysis. The spleen lymphocyte proliferation assays were measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide (MTT) method. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. Chickens immunized with pcDNA-3-1E-linker-mChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen following two immunizations compared with chickens in other groups (P<0.01). In comparison with pcDNA3.1-immunized and control groups, lymphocyte proliferation, percentage of CD8α+cell, and levels of ChIL-2 and ChIFN-γ transcripts in the group immunized with pcDNA-3-1E-linker-mChIL-15 were significantly increased on day 6 following challenge (P<0.05,P<0.01, andP<0.01, resp.). Our data suggested that the fusion antigen 3-1E-linker-mChIL-15 could be a potential candidate forE. acervulinavaccine development.

scholarly journals Peste des Petits Ruminants Virus-Like Particles Induce a Potent Humoral and Cellular Immune Response in Goats

Viruses ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 918 ◽  
Author(s):  
Yan ◽  
Banadyga ◽  
Zhao ◽  
Zhao ◽  
Schiffman ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Vaccine Development ◽  
Expression System ◽  
Small Ruminants ◽  
Economic Losses ◽  
Potential Candidate ◽  
Peste Des Petits Ruminants ◽  
Ifn Γ ◽  
Cellular Immune

Peste des petits ruminants is a highly contagious acute or subacute disease of small ruminants caused by the peste des petits ruminants virus (PPRV), and it is responsible for significant economic losses in animal husbandry. Vaccination represents the most effective means of controlling this disease, with virus-like particle (VLP) vaccines offering promising vaccine candidates. In this study, a PPRV VLP-based vaccine was developed using a baculovirus expression system, allowing for the simultaneous expression of the PPRV matrix (M), hemagglutinin (H), fusion (F) and nucleocapsid (N) proteins in insect cells. Immunization of mice and goats with PPRV VLPs elicited a robust neutralization response and a potent cellular immune response. Mouse studies demonstrated that VLPs induced a more robust IFN-γ response in CD4+ and CD8+ T cells than PPRV Nigeria 75/1 and recruited and/or activated more B cells and dendritic cells in inguinal lymph nodes. In addition, PPRV VLPs induced a strong Th1 class response in mice, as indicated by a high IgG2a to IgG1 ratio. Goat studies demonstrated that PPRV VLPs can induce the production of antibodies specific for F and H proteins and can also stimulate the production of virus neutralizing antibodies to the same magnitude as the PPRV Nigeria 75/1 vaccine. Higher amounts of IFN-γ in VLP-immunized animal serum suggested that VLPs also elicited a cellular immune response in goats. These results demonstrated that VLPs elicit a potent immune response against PPRV infection in small ruminants, making PPRV VLPs a potential candidate for PPRV vaccine development.

scholarly journals Evaluation of cellular immune response in Golden Hamsters experimentally infected with Leishmania donovani comparing with cellular immune response against chicken Red Blood Cells.

2012 ◽  
Vol 9 (1) ◽  
pp. 23-30
Author(s):  
Baghdad Science Journal
Keyword(s):  
Immune Response ◽  
Red Blood Cells ◽  
Skin Test ◽  
Blood Cells ◽  
Cellular Immune Response ◽  
Leishmania Donovani ◽  
Cellular Immune ◽  
And Control ◽  
Delayed Type Of Hypersensitivity

The Evaluation of the immune response in Golden Hamsters experimentally infected with Leishmania donovani was determined in this study, particularly, the cellular immune response. Follow up has maintained to determine the Delayed Type of Hypersensitivity using skin test both in infected and control lab animals. Chicken red blood cells were used as a parameter to evaluate the immune system; they are dull and have the ability of immunization. Two concentrations of chicken R.B.C were examined to determine which gives the higher titration in Hamsters and those were 1.5 X 109 cell/ml and 3 X 109 cell/ml , the second concentration gave the maximum titration where then used in this work. After sensitization with Chicken R.B.C for both infected and control groups, delayed type of hypersensitivity has been used against Leishmania donovani antigen and 4 days of follow up were adopted and they were (14, 30, 60, 90) day after infection. Results showed that skin test against both antigens ( L.donovani and chicken R.B.C) was significantly higher than normal at the first day of follow up ( day 14) then gradual decreasing were noticed till the last day of follow up (90). This can indicate that the infection with L.donovani activated the immune response at the beginning of infection, then leads to cellular immune suppression against both L.donovani antigen and chicken R.B.C., so that this immunosuppression is not specific.

Improved Cellular Immune Response Elicited by a Ubiquitin-Fused DNA Vaccine AgainstMycobacterium tuberculosis

2012 ◽  
Vol 31 (4) ◽  
pp. 489-495 ◽  
Author(s):  
Qingmin Wang ◽  
Chengxiang Lei ◽  
Hui Wan ◽  
Qiuhong Liu
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Cellular Immune

Attenuated nef DNA vaccine construct induces cellular immune response: role in HIV-1 multiprotein vaccine

2003 ◽  
Vol 89 (2-3) ◽  
pp. 207-214 ◽  
Author(s):  
Biswanath Majumder ◽  
Benjamin Gray ◽  
Sean McBurney ◽  
Todd M. Schaefer ◽  
Tzvete Dentchev ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Cellular Immune ◽  
Hiv 1

Experimental Leishmania major Infection Suppresses HIV-1 DNA Vaccine Induced Cellular Immune Response

2004 ◽  
Vol 177 (3) ◽  
pp. 185-188 ◽  
Author(s):  
Tara M. Robinson ◽  
Robin Nelson ◽  
David Artis ◽  
Phillip Scott ◽  
Jean D. Boyer
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Leishmania Major ◽  
Cellular Immune ◽  
Hiv 1

scholarly journals A novel adjuvant, the general opioid antagonist naloxone, elicits a robust cellular immune response for a DNA vaccine

2009 ◽  
Vol 21 (3) ◽  
pp. 217-225 ◽  
Author(s):  
A. Jamali ◽  
M. Mahdavi ◽  
Z. M. Hassan ◽  
F. Sabahi ◽  
M. J. Farsani ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Opioid Antagonist ◽  
Cellular Immune
Sign in / Sign up

Export Citation Format

Share Document