Two-Dimensional IgE-Binding Spectrum of Japanese Cedar (Cryptomeria japonica) Pollen Allergens

2004 ◽  
Vol 133 (2) ◽  
pp. 125-135 ◽  
Author(s):  
Takashi Fujimura ◽  
Seiko Shigeta ◽  
Seiji Kawamoto ◽  
Tsunehiro Aki ◽  
Masanori Masubuchi ◽  
...  
Keyword(s):  
Cryptomeria Japonica ◽  
Japanese Cedar ◽  
Two Dimensional ◽  
Pollen Allergens ◽  
Ige Binding

A new method of counting airborne Japanese cedar (Cryptomeria japonica) pollen allergens by immunoblotting

Allergy ◽  
1993 ◽  
Vol 48 (2) ◽  
pp. 94-98 ◽  
Author(s):  
Y. Takahashi ◽  
T. Nagoya ◽  
M. Watanabe ◽  
S. Inouye ◽  
M. Sakaguchi ◽  
...  
Keyword(s):  
Cryptomeria Japonica ◽  
Japanese Cedar ◽  
New Method ◽  
Pollen Allergens

IgE-reactivity to major Japanese cedar (Cryptomeria japonica) pollen allergens (Cry j 1 and Cry j 2) by ELISA in dogs with atopic dermatitis

2000 ◽  
Vol 74 (3-4) ◽  
pp. 263-270 ◽  
Author(s):  
Kenichi Masuda ◽  
Hajime Tsujimoto ◽  
Shunsuke Fujiwara ◽  
Keigo Kurata ◽  
Atsuhiko Hasegawa ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Cryptomeria Japonica ◽  
Japanese Cedar ◽  
Pollen Allergens ◽  
Ige Reactivity ◽  
Cry J 2

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cryptomeria Japonica ◽  
Fluorescent Protein ◽  
Japanese Cedar ◽  
Targeted Mutagenesis ◽  
Embryogenic Tissue ◽  
Breeding Period ◽  
Single Mutation ◽  
Knock Out ◽  
Guide Rna

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.

Constructing a hybrid molecule with low capacity of IgE binding from Chenopodium album pollen allergens

2012 ◽  
Vol 144 (1-2) ◽  
pp. 67-77 ◽  
Author(s):  
Hamid Reza Nouri ◽  
Abdolreza Varasteh ◽  
Fatemeh Vahedi ◽  
Jamshidkhan Chamani ◽  
Danial Afsharzadeh ◽  
...  
Keyword(s):  
Chenopodium Album ◽  
Hybrid Molecule ◽  
Pollen Allergens ◽  
Ige Binding
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