The Tat Protein of the Caprine Arthritis Encephalitis Virus Interacts with the Notch2 EGF-Like Repeats and the Epithelin/Granulin Precursor

Intervirology ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Nitza Shoham ◽  
Limor Cohen ◽  
Arnona Gazit ◽  
Abraham Yaniv
Keyword(s):  
Encephalitis Virus ◽  
Tat Protein ◽  
Caprine Arthritis Encephalitis Virus

scholarly journals Maedi-Visna Virus and Caprine Arthritis Encephalitis Virus Genomes Encode a Vpr-Like but No Tat Protein

2003 ◽  
Vol 77 (17) ◽  
pp. 9632-9638 ◽  
Author(s):  
Stéphanie Villet ◽  
Baya Amel Bouzar ◽  
Thierry Morin ◽  
Gérard Verdier ◽  
Catherine Legras ◽  
...  
Keyword(s):  
Protein Structures ◽  
Activation Function ◽  
Encephalitis Virus ◽  
Accessory Protein ◽  
Tat Protein ◽  
Caprine Arthritis Encephalitis Virus ◽  
Transfected Cells ◽  
Visna Virus ◽  
Maedi Visna Virus ◽  
Hiv 1

ABSTRACT A small open reading frame (ORF) in maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV) was initially named “tat” by analogy with a similarly placed ORF in the primate lentiviruses. The encoded “Tat” protein was ascribed the function of up regulation of the viral transcription from the long terminal repeat (LTR) promoter, but we have recently reported that MVV and CAEV Tat proteins lack trans-activation function activity under physiological conditions (S. Villet, C. Faure, B. Bouzar, G. Verdien, Y. Chebloune, and C. Legras, Virology 307:317-327, 2003). In the present work, we show that MVV Tat localizes to the nucleus of transfected cells, probably through the action of a nuclear localization signal in its C-terminal portion. We also show that, unlike the human immunodeficiency virus (HIV) Tat protein, MVV Tat was not secreted into the medium by transfected human or caprine cells in the absence of cell lysis but that, like the primate accessory protein Vpr, MVV and CAEV Tat proteins were incorporated into viral particles. In addition, analysis of the primary protein structures showed that small-ruminant lentivirus (SRLV) Tat proteins are more similar to the HIV type 1 (HIV-1) Vpr protein than to HIV-1 Tat. We also demonstrate a functional similarity between the SRLV Tat proteins and the HIV-1 Vpr product in the induction of a specific G2 arrest of the cell cycle in MVV Tat-transfected cells, which increases the G2/G1 ratio 2.8-fold. Together, these data strongly suggest that the tat ORF in the SRLV genomes does not code for a regulatory transactivator of the LTR but, rather, for a Vpr-like accessory protein.

Characterization of cDNAs Species Encoding the Tat Protein of Caprine Arthritis Encephalitis Virus

Virology ◽  
1994 ◽  
Vol 204 (2) ◽  
pp. 828-834 ◽  
Author(s):  
Hagar Kalinski ◽  
Pnina Mashiah ◽  
Dagan Rotem ◽  
Yael Orzech ◽  
Levana Sherman ◽  
...  
Keyword(s):  
Encephalitis Virus ◽  
Tat Protein ◽  
Caprine Arthritis Encephalitis Virus

scholarly journals Caprine Arthritis Encephalitis Virus Is Associated with Renal Lesions

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1051
Author(s):  
Brian G. Murphy ◽  
Diego Castillo ◽  
Asli Mete ◽  
Helena Vogel ◽  
Dayna Goldsmith ◽  
...  
Keyword(s):  
Renal Injury ◽  
Binding Sites ◽  
Encephalitis Virus ◽  
Clinical Consequence ◽  
Viral Isolate ◽  
Viral Promoter ◽  
Caprine Arthritis Encephalitis Virus ◽  
Renal Lesions ◽  
Cardiac Lesions ◽  
The Central Nervous System

Caprine arthritis encephalitis virus (CAEV) is a monocyte/macrophage-tropic lentivirus that primarily infects goats resulting in a well-recognized set of chronic inflammatory syndromes focused on the joint synovium, tissues of the central nervous system, pulmonary interstitium and mammary gland. Clinically affected animals generally manifest with one or more of these classic CAEV-associated tissue lesions; however, CAEV-associated renal inflammation in goats has not been reported in the peer-reviewed literature. Here we describe six goats with chronic, multisystemic CAEV infections in conjunction with CAEV-associated renal lesions. One of the animals had CAEV antigen-associated thrombotic arteritis resulting in infarction of both the kidney and heart. These goats had microscopic evidence of inflammatory renal injury (interstitial nephritis) with detectable renal immunolabeling for CAEV antigen in three of six animals and amplifiable proviral sequences consistent with CAEV in all six animals. Cardiac lesions (vascular, myocardial or endocardial) were also identified in four of six animals. Within the viral promoter (U3) region, known transcription factor binding sites (TFBSs) were generally conserved, although one viral isolate had a duplication of the U3 A region encoding a second gamma-activated site (GAS). Despite the TFBS conservation, the isolates demonstrated a degree of phylogenetic diversity. At present, the clinical consequence of CAEV-associated renal injury is not clear.

scholarly journals Genetic diversity of small-ruminant lentiviruses: characterization of Norwegian isolates of Caprine arthritis encephalitis virus

2006 ◽  
Vol 87 (3) ◽  
pp. 573-580 ◽  
Author(s):  
Britt Gjerset ◽  
Anne K. Storset ◽  
Espen Rimstad
Keyword(s):  
Genetic Diversity ◽  
Small Ruminant ◽  
Genomic Sequence ◽  
Sequence Divergence ◽  
Encephalitis Virus ◽  
Surface Glycoprotein ◽  
Group Designation ◽  
Variable Regions ◽  
Caprine Arthritis Encephalitis Virus ◽  
Small Ruminant Lentiviruses

Small-ruminant lentiviruses (SRLVs), including Caprine arthritis encephalitis virus (CAEV) in goats and maedi-visna virus (MVV) in sheep, are lentiviruses that, despite overall similarities, show considerable genetic variation in regions of the SRLV genome. To gain further knowledge about the genetic diversity and phylogenetic relationships among field isolates of SRLVs occurring in geographically distinct areas, the full-length genomic sequence of a CAEV isolate (CAEV-1GA) and partial env sequences obtained from Norwegian CAEV-infected goats were determined. The genome of CAEV-1GA consisted of 8919 bp. Alignment studies indicated significant diversity from published SRLV sequences. Deletions and hypervariability in the 5′ part of the env gene have implications for the size of the proposed CAEV-1GA Rev protein and the encoded surface glycoprotein (SU). The variable regions in the C-terminal part of SU obtained from Norwegian CAEV isolates demonstrate higher sequence divergence than has been described previously for SRLVs. Phylogenetic analysis based on SU sequences gives further support for a unique group designation. The results described here reveal a distant genetic relationship between Norwegian CAEV and other SRLVs and demonstrate that there is more geographical heterogeneity among SRLVs than reported previously.

scholarly journals High prevalence of caprine arthritis encephalitis virus (CAEV) in Taiwan revealed by large-scale serological survey

2017 ◽  
Vol 79 (2) ◽  
pp. 273-276 ◽  
Author(s):  
Wei-Cheng YANG ◽  
Hui-Yu CHEN ◽  
Chi-Young WANG ◽  
Hung-Yu PAN ◽  
Cheng-Wei WU ◽  
...  
Keyword(s):  
Large Scale ◽  
Encephalitis Virus ◽  
Serological Survey ◽  
Caprine Arthritis Encephalitis Virus ◽  
High Prevalence
Sign in / Sign up

Export Citation Format

Share Document