Effects of Topical Mydriatics and Vasoconstrictors on Prostaglandin-E2-Induced Aqueous Flare Elevation in Pigmented Rabbits

2003 ◽  
Vol 35 (5) ◽  
pp. 256-260 ◽  
Author(s):  
Yoriko Hayasaka ◽  
Seiji Hayasaka ◽  
Xue-Yun Zhang ◽  
Yasunori Nagaki
Keyword(s):  
Prostaglandin E ◽  
Aqueous Flare ◽  
Pigmented Rabbits

Effects of Oral Administration of Gardeniae fructus Extract and Intravenous Injection of Crocetin on Lipopolysaccharide- and Prostaglandin E2-induced Elevation of Aqueous Flare in Pigmented Rabbits

2003 ◽  
Vol 31 (05) ◽  
pp. 729-738 ◽  
Author(s):  
Yasunori Nagaki ◽  
Seiji Hayasaka ◽  
Tomohiro Abe ◽  
Xue-Yun Zhang ◽  
Yoriko Hayasaka ◽  
...  
Keyword(s):  
Oral Administration ◽  
Intravenous Injection ◽  
Area Under The Curve ◽  
Prostaglandin E ◽  
Dependent Manner ◽  
Aqueous Flare ◽  
Laser Flare ◽  
Coptidis Rhizoma ◽  
Phellodendri Cortex ◽  
Pigmented Rabbits

The purpose of this study was to evaluate the effects of extracts of Coptidis rhizoma, Phellodendri cortex and Gardeniae fructus, which are medicinal herbs in Orengedoku-to (Huanglin-Jie-Du-Tang in Chinese), and crocetin (a major component of Gardeniae fructus) on experimental elevation of aqueous flare in pigmented rabbits. To produce aqueous flare elevation, 0.5 μg/kg lipopolysaccharide (LPS) was injected into the ear vein, or prostaglandin E2 (PGE2) 25 μg/ml, was applied to the cornea by means of a glass cylinder. Animals were pretreated by oral administration of 150 g/day of food containing 0.15% (w/w) extract powder of Coptidis rhizoma, 0.10% (w/w) extract powder of Phellodendri cortex or 0.15% (w/w) extract powder of Gardeniae fructus for 4 days, or by intravenous injection of crocetin, 0.3, 3, 30 or 300 μg/kg, 30 minutes before aqueous flare elevation. Aqueous flare was measured with a laser flare-cell meter. Aqueous flare intensity was expressed as the area under the curve (AUC) in arbitrary units. The AUC of LPS- and PGE2-induced aqueous flare elevation was 4685 and 1386 arbitrary units, respectively. Pretreatment by oral administration of 0.15% (w/w) extract of Coptidis rhizoma or 0.10% (w/w) extract of Phellodendri cortex did not inhibit LPS-induced aqueous flare elevation. Pretreatment by oral administration of 0.15% extract of Gardeniae fructus suppressed LPS-induced aqueous flare elevation (AUC: 1411 arbitrary units). Pretreatment by intravenous injection of 3, 30 or 300 μg/kg of crocetin-inhibited LPS-induced aqueous flare elevation in a dose-dependent manner. Pretreatment with 3 or 30 μg/kg of crocetin did not inhibit PGE2-induced aqueous flare elevation, but 300 μg/kg of crocetin inhibited PGE2-induced aqueous flare elevation (AUC: 918 arbitrary units).

Effect of Topical Iganidipine on Experimental Elevation of Aqueous Flare Induced by Prostaglandin E2 and EP Agonists in Pigmented Rabbits

2002 ◽  
Vol 34 (4) ◽  
pp. 195-199 ◽  
Author(s):  
Shuichiro Yanagisawa ◽  
Seiji Hayasaka ◽  
Xue-Yun Zhang ◽  
Yoriko Hayasaka ◽  
Yasunori Nagaki
Keyword(s):  
Prostaglandin E ◽  
Aqueous Flare ◽  
Pigmented Rabbits

Effect of Topical Betaxolol on the Acute Rise of Aqueous Flare Induced by Highly Selective Agonists for Prostaglandin E2 Receptor Subtypes in Pigmented Rabbits

2002 ◽  
Vol 34 (1) ◽  
pp. 48-50 ◽  
Author(s):  
Shuichiro Yanagisawa ◽  
Seiji Hayasaka ◽  
Xue-Yun Zhang ◽  
Yoriko Hayasaka ◽  
Yasunori Nagaki ◽  
...  
Keyword(s):  
Prostaglandin E ◽  
Receptor Subtypes ◽  
Aqueous Flare ◽  
Selective Agonists ◽  
Pigmented Rabbits

Effects of Isopropyl Unoprostone, Latanoprost, and Prostaglandin E2 on Acute Rise of Aqueous Flare in Pigmented Rabbits

2002 ◽  
Vol 34 (2) ◽  
pp. 90-93 ◽  
Author(s):  
Xue-Yun Zhang ◽  
Seiji Hayasaka ◽  
Yoriko Hayasaka ◽  
Shuichiro Yanagisawa ◽  
Yasunori Nagaki
Keyword(s):  
Prostaglandin E ◽  
Aqueous Flare ◽  
Pigmented Rabbits

Effects of Topical Clonidine on Prostaglandin-E2-Induced Aqueous Flare Elevation in Pigmented Rabbits

2000 ◽  
Vol 32 (5) ◽  
pp. 210-214
Author(s):  
Kazuhiko Watanabe ◽  
Seiji Hayasaka ◽  
Shigeyoshi Hiraki ◽  
Masayuki Matsumoto ◽  
Chiharu Kadoi ◽  
...  
Keyword(s):  
Prostaglandin E ◽  
Aqueous Flare ◽  
Pigmented Rabbits ◽  
Topical Clonidine

Effects of Orengedoku-to and Senkanmeimoku-to, Traditional Herbal Medicines, on the Experimental Herbal Medicines, on the Experimental Elevation of Aqueous Flare in Pigmental Rabbits

2001 ◽  
Vol 29 (01) ◽  
pp. 141-147 ◽  
Author(s):  
Yasunoru Nagaki ◽  
Seiji Hayasaka ◽  
Chiharu Kadoi ◽  
Masayuki Matsumoto ◽  
Nobuo Nakamura ◽  
...  
Keyword(s):  
Oral Administration ◽  
Herbal Medicines ◽  
Glass Cylinder ◽  
Aqueous Flare ◽  
Laser Flare ◽  
Pigmented Rabbits

We investigated the effects of Orengedoku-to (Huanglian-Jie-Du-Tang in Chinese) and Senkanmeimoku-to (Xygan-Ming-Mu-Tang in Chinese), traditional herbal medicines, on experimantal elevation of aqueous flare in pigmented rabbits. To produce the elevation of aqueous flare in rabbits, prostagalandin E2 (PGE2) was applied to the cornea with use of a glass cylinder, or lipopolysaccharides (LPS) were injected into the ear vein. Animals were pretreated by the oral administration of 150 g/day of food containing 0.7%, 0.2% or 0.07% (w/w) Orengedoku-to, or 2%, 0.6% or 0.2% (w/w) Senkanmeimoku-to for 5 days. Aquenous flare was measured with a laser flare-cell meter. Pretreatment with 0.7% or 0.2% Orengedoku-to and 2% Senkanmeimoku-to did suppress significantly (P < 0.05) elevation of aqueous flare induced by PGE2. Pretreatment with 0.7% or 0.2% Orengedoku-to and 2% or 0.6% Senkanmeimoku-to significantly suppressed (P < 0.001) elevation of aqueous flare induced by LPS. It is possible that Orengedoku-to and Senkanmeimoku-to may migrate some forms of uveitis.

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