Percutaneous Ultrasound-Guided Injection of the Trachea in Fetal Sheep: A Novel Technique to Target the Fetal Airways

2003 ◽  
Vol 18 (5) ◽  
pp. 385-390 ◽  
Author(s):  
A.L. David ◽  
D.M. Peebles ◽  
L. Gregory ◽  
M. Themis ◽  
T. Cook ◽  
...  
Keyword(s):  
Ultrasound Guided ◽  
Fetal Sheep ◽  
Novel Technique

An ultrasound-guided procedure to administer a label of DNA synthesis into fetal sheep

1999 ◽  
Vol 11 (5) ◽  
pp. 303 ◽  
Author(s):  
Paul L. Greenwood ◽  
Ramona M. Slepetis ◽  
Alan W. Bell ◽  
John W. Hermanson
Keyword(s):  
Bolus Dose ◽  
S Phase ◽  
Fetal Weight ◽  
Ultrasound Guided ◽  
Short Term ◽  
Fetal Sheep ◽  
Novel Technique ◽  
Cellular Events ◽  
High Degree

A novel technique was developed to deliver a bolus dose of a DNA label into the peritoneal cavity of fetal sheep at 85–130 days gestation. Use of markers to identify the site of injection in fetuses from litters up to quadruplets, and immunohistochemistry to detect the DNA label, 5-bromo-2¢-deoxyuridine (BrdU), confirmed the procedure was successful in 85% of cases. Duration of the procedure was (mean SD) 44 16 min, and recovery from anaesthesia was rapid and uneventful in all cases. Fetal weight was estimated with a high degree of accuracy (residual standard deviation (RSD) = 297 g and r 2 = 0.93, P<0.001) and the dose of label administered (110 33 mg BrdU/kg fetal weight) was adequate in all cases. BrdU detected in fetal nuclei following injection into amniotic fluid highlights the need for positive identification of the injection site in timed, short-term studies, and suggests potential to further develop the technique to investigate cellular events in fetal sheep younger than 85 days of gestation. The results demonstrate that the procedure can be used to determine in vivo whether or not nuclei have entered the S-phase of the cell cycle.

Ultrasound guided blood sampling from fetal sheep

1989 ◽  
Vol 40 (2) ◽  
pp. 401 ◽  
Author(s):  
JP Newnham ◽  
RW Kelly ◽  
P Boyne ◽  
SE Reid
Keyword(s):  
Ultrasound Guidance ◽  
Needle Aspiration ◽  
Blood Sampling ◽  
Ultrasound Guided ◽  
Fetal Sheep ◽  
Arterial Blood Gas ◽  
Operator Experience ◽  
Arterial Blood ◽  
Fetal Death Rate ◽  
Repeated Sampling

The feasibility of obtaining fetal blood samples by needle aspiration under ultrasound guidance was assessed in 32 fetal sheep from 101-1 36 days' gestation. Seventy-six attempts at blood sampling were made, of which all but two were successful. The overall fetal death rate resulting from this procedure was 9.2%. However, with increasing operator experience and with advancing gestational age, 95.5% of fetuses survived the procedure. Repeated sampling from individual fetuses had minimal effects on arterial blood gas values. Ultrasound guided blood sampling now provides an alternative to chronic catheterization as a basis for some types of research with fetal sheep.

scholarly journals Wire- and Ultrasound-Guided Localization: A Novel Technique for Excision of Nonpalpable Breast Tumors

2020 ◽  
Vol 14 ◽  
pp. 117822342093806
Author(s):  
Siddhant Khare ◽  
Tulika Singh ◽  
Irrinki Santosh ◽  
Ishita Laroiya ◽  
Gurpreet Singh
Keyword(s):  
Breast Cancer ◽  
Preoperative Diagnosis ◽  
Standard Technique ◽  
Positive Margin ◽  
Breast Lesions ◽  
Benign Lesions ◽  
Ultrasound Guided ◽  
Novel Technique ◽  
Widespread Acceptance ◽  
Nonpalpable Breast Lesions

Background: Excision of nonpalpable breast lesions requires intraoperative guidance. Wire-guided localization and intraoperative ultrasounds have been used successfully but suffer from some disadvantages. We describe a new modification of the standard technique using a combination of preoperative ultrasound in conjunction with standard wire-guided localization. Methods: Wire and ultrasound-guided localization (WUGL) technique was used for the excision of nonpalpable breast lesions. Results: Sixty-nine patients with nonpalpable breast lesions were subjected to excision using WUGL, out of whom 63 patients had a preoperative diagnosis of invasive/noninvasive breast cancer. Six patients had a preoperative diagnosis of benign lesions, out of which 3 patients were converted to invasive breast cancer on final pathology. Only 1 patient had positive margin. Conclusions: WUGL is a technique that uses a combination of well-accepted and easily available techniques. It has given good results and has the potential for widespread acceptance in resource-constrained situations.

Ultrasound guided placement of an occlusive balloon in the trachea of fetal sheep

2004 ◽  
Vol 191 (6) ◽  
pp. S166
Author(s):  
Anna David ◽  
Boaz Weisz ◽  
Donald Peebles ◽  
Xenia Roubliova ◽  
Dany Perocheau ◽  
...  
Keyword(s):  
Ultrasound Guided ◽  
Fetal Sheep

scholarly journals NOVEL TECHNIQUE OF DUPLEX ULTRASOUND GUIDED CANNULATION OF COMPLETE FLUSH OCCLUSION OF SFA AND BYPASS GRAFT

2010 ◽  
Vol 55 (10) ◽  
pp. A216.E2046 ◽  
Author(s):  
Nelson L. Bernardo ◽  
Gabriel Maluenda ◽  
Manuel A. Gonzalez ◽  
Sara D. Collins ◽  
Vishal Gupta ◽  
...  
Keyword(s):  
Bypass Graft ◽  
Duplex Ultrasound ◽  
Ultrasound Guided ◽  
Novel Technique
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