scholarly journals Comparative Investigation of the Antimicrobial Activity of PADMA 28 and Selected European Herbal Drugs

2002 ◽  
Vol 9 (6) ◽  
pp. 346-351 ◽  
Author(s):  
A. Weseler ◽  
R. Saller ◽  
J. Reichling
Keyword(s):  
Antimicrobial Activity ◽  
Comparative Investigation ◽  
Herbal Drugs ◽  
Padma 28

Comparative Study of the Biologically Active Substances Composition and Content in Meadowsweet (Filipendula ulmaria (L.) Maxim) Crude Herbal Drugs (Herb, Leafs, Flowers) of Russian Origin

2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Dmitry Olegovich Bokov ◽  
Tatyana Yuryevna Kovaleva ◽  
Valentina Alekseevna Ermakova ◽  
Daria Aleksandrovna Trashchenkova ◽  
Ekaterina Anatolievna Dorovskih ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Neurotropic Activity ◽  
Comparative Investigation ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Herbal Drugs ◽  
Filipendula Ulmaria ◽  
Pharmacopoeial Monograph ◽  
Total Extractives ◽  
Layer Chromatography

Filipendula ulmaria (L.) Maxim. (Meadowsweet) is known in traditional medicine as anti-inflammatory, wound-healing,astringent and antibacterial remedy. However recent studies show that it also has neurotropic activity. In Russia meadowsweet flowers are used as crude herbal drugs (temporary pharmacopoeial monograph 42-1777-87), also leafs and herb are used in the traditional medicine. Objective of the study was to carry out comparative investigation of composition and content of major biologically active compounds (BAC) in Filipendula ulmaria herb, flowers and leafs by thin-layer chromatography, differential spectrophotometry with aluminum chloride reagent (total flavonoids in terms of rutoside), gravimetry (total extractives, extracted by water), permanganatometric titration (total tannins in terms of tannin). Rutoside, tannin, gallic acid and salicylic acid were identified in Filipendula ulmaria herb, flowers and leafs by TLC. Also we analyzed content of substances extracted by water, flavonoids and tannins. Total extractives, extracted by water in F. ulmaria herb is 13.12±0.10%, in leafs – 13.98±0.37%, in flowers – 18.09±0.17%. Total tannins in F. ulmaria herb is 11.87±0.47%, in leafs – 12.06±0.18%, in flowers – 12.26±0.29%. Total flavonoids in F. ulmaria herb 4.34±0.17%, in leafs – 6.98±0.23%, in flowers – 11.75±0.57%. The obtained data will be used for development of a pharmacopoeial monograph project "Filipendula ulmaria (L.) Maxim., herba" for inclusion in the State Pharmacopoeia of the Russian Federation.

Formulation and evaluation of herbal gel for management of mouth ulcers

2021 ◽  
Vol 8 (3) ◽  
pp. 226-230
Author(s):  
K. Upadhye ◽  
K Charde ◽  
G Dixit ◽  
S Bakhle
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extract ◽  
Research Work ◽  
Physical Appearance ◽  
Gelling Agent ◽  
Herbal Drugs ◽  
Herbal Extracts ◽  
Common Condition ◽  
Ph Values ◽  
Mouth Ulcer

: or mouth ulcers is the most common condition that we encounter. Clinically the lesions are single or multiple superficial and deep sealed and are associated with microbial invasions.: This study was conducted with the aim of evaluating the effectiveness of herbal drugs for treatment of In the research work, mouth ulcer gels were formulated incorporating the extracts of as and using 934 as the gelling agent. Seven batches were formulated by varying the concentration of the herbal ingredients (F1 to F7)The prepared formulations were evaluated for various parameters like physical appearance, pH, Homogeneity and antimicrobial activity against fungi and bacteria. The antimicrobial activity was also compared with a marketed gel formulation. All the prepared formulation using different concentration of plant extract showed the pH values in between 6.1±0.2 to 7.0±0.1. The values ranged between the 5.0 to 8.0 cm. Out of all the formulations, formulation F7 containing all the three herbal extracts showed a goodand very promising antimicrobial activity comparable with a marketed gel. Thus stable, effective gels containing herbal ingredients for management of mouth ulcers can be developed.

scholarly journals Comparative Analysis of Nutritional Constituents, Antioxidant and Antimicrobial Activities of Some Common Vegetable Wastes

2021 ◽  
Vol 9 (1) ◽  
pp. 62-74
Author(s):  
Swagata Biswas ◽  
Pranabesh Ghosh ◽  
Alolika Dutta ◽  
Maitrayee Biswas ◽  
Sirshendu Chatterjee
Keyword(s):  
Antimicrobial Activity ◽  
Cucumis Sativus ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Comparative Investigation ◽  
Diffusion Method ◽  
Banana Peel ◽  
Lagenaria Siceraria ◽  
Primary And Secondary Metabolites ◽  
Nutritional Constituents

Vegetables are intrinsic to a healthy diet. But the peels are discarded as food waste, unknowing of their potential as the source of bioactive compounds. The study aims to find the nutritional constituents, antioxidant and antimicrobial activity of these food wastes. Here we make a comparative investigation among the five underutilized vegetable parts namely, Solanum tuberosum (Potato peel), Cucumis sativus (Cucumber peel), Musa acuminata (Unripe Banana peel), Brassica oleracea (Cauliflower stem), Lagenaria siceraria (Bottle gourd peel). After the primary screening, including proximate and qualitative analysis, the quantification of primary and secondary metabolites as well as minerals was estimated by different standard methods. The antioxidant potential was evaluated by both DPPH and H2O2 radical scavenging assays. Antimicrobial activity was analyzed by Kirby Bauer disc diffusion method against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) strains of bacteria compared with positive and negative controls. From the proximate analysis, the highest moisture and water content were found in the peel sample of Lagenaria siceraria. Out of ten qualitative tests, protein, fatty acid, flavonoid, alkaloid and xanthoprotein were detected in all five samples. Among the quantitative estimations, Lagenaria siceraria showed the highest amount of polysaccharides (85.82±0.12 mg DE/g DW), ascorbic acid (2.48±0.14 mg AAE/g FW), thiamine (24.46±0.13 mg TE/g DW), polyphenols (86.36±0.10 mg GAE/g DW), flavonoids (49.59±0.07 mg QE/g DW), minerals like K, Ca and Mg and 72.35±0.40% inhibition by DPPH. Simultaneously a significant amount of protein (63.59±0.13 mg BSAE/g FW), the amino acid (7.84±0.02 mg AAE/g DW), minerals like Na, Zn and B and 88.76±0.10% inhibition by H2O2 were found in Cucumis sativus. At a higher concentration, all samples were exhibited significant antimicrobial activity which laid out a strong correlation with previously screened phytonutrients and antioxidants. The overall findings suggested that these underutilized vegetable parts can be utilized in the processing of potential functional foods as well as pharmaceuticals rather than thrown out as agro-waste.

scholarly journals Growth and pathogenesis of Enteropathogenic E.coli as affected by bacteriocin produced and purified from Lactobacillus isolates with or without Quercus infectoria

2012 ◽  
Vol 36 (0E) ◽  
pp. 1-7
Author(s):  
Hassan A. Abdul-Ratha
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Herbal Drugs ◽  
Bacteriocin Activity ◽  
Recovery Yield ◽  
Quercus Infectoria

The antimicrobial activity of Lactobacilli has been widely exploited for prevention offood –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially inchildren, because of bacteriocin activity and the importance of herbal drugs, hence this studywas designed to evaluate the synergistic effect of plant extract and bacteriocin produced byLactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli.1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C.2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min andthen purified by two steps method including extraction with n-butanol followed by gelfiltration chromatography on Sepharose 6B column. The results showed that the specificactivity was 1600 AU/mg protein with 8 purification folds and 12% recovery yield.3. The antibacterial activity of Quercus infectoria with concentration 300 mg/ml wasshowed highly antibacterial activity in vitro and in vivo.4. The result showed synergistic effect of Plantaricin with Quercus infectoria extract afterexperimental infection that induced by orally dosing with Escherichia coli in vivo. A result ofhistopathological study was recorded recovery of tissue.

GC-MS characterization of n-hexane soluble fraction from dandelion (Taraxacum officinale Weber ex F.H. Wigg.) aerial parts and its antioxidant and antimicrobial properties

2018 ◽  
Vol 73 (1-2) ◽  
pp. 41-47 ◽  
Author(s):  
Ivan Ivanov ◽  
Nadezhda Petkova ◽  
Julian Tumbarski ◽  
Ivayla Dincheva ◽  
Ilian Badjakov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Soluble Fraction ◽  
Antimicrobial Properties ◽  
Taraxacum Officinale ◽  
Comparative Investigation ◽  
Biologically Active ◽  
Ion Current ◽  
Aerial Parts ◽  
Pharmaceutical Industries

AbstractA comparative investigation of n-hexane soluble compounds from aerial parts of dandelion (Taraxacum officinaleWeber ex F.H. Wigg.) collected during different vegetative stages was carried out. The GC-MS analysis of the n-hexane (unpolar) fraction showed the presence of 30 biologically active compounds. Phytol [14.7% of total ion current (TIC)], lupeol (14.5% of TIC), taraxasteryl acetate (11.4% of TIC), β-sitosterol (10.3% of TIC), α-amyrin (9.0% of TIC), β-amyrin (8.3% of TIC), and cycloartenol acetate (5.8% of TIC) were identified as the major components in n-hexane fraction. The unpolar fraction exhibited promising antioxidant activity – 46.7 mmol Trolox equivalents/g extract (determined by 1,1-diphenyl-2-picrylhydrazyl method). This fraction demonstrated insignificant antimicrobial activity and can be used in cosmetic and pharmaceutical industries.

scholarly journals The Study of the Composition of Chloroform Fraction of Anemone nemorosa L.

2019 ◽  
Vol 7 (2) ◽  
pp. 153-159
Author(s):  
Oksana Khropot ◽  
Yulian Konechnyi ◽  
Nataliya Polish ◽  
Roksolana Konechna ◽  
Yehor Bazavluk ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Biologically Active ◽  
Volatile Fraction ◽  
Chloroform Fraction ◽  
Herbal Drugs ◽  
Gram Negative ◽  
Lipophilic Extract ◽  
Anemone Nemorosa ◽  
Active Substances

Expanding the range of herbal drugs is among the priorities of the modern pharmacy. The analysis presented in this article is drawn from a broader qualitative study examining the composition of Anemone nemorosa L. chloroform fraction. The study revealed 38 compounds, and 32 of them were identified. The investigated lipophilic extract has an antimicrobial activity both in terms of gram-positive and gram-negative microorganisms. The maximal antimicrobial action of the investigated extract was shown to Escherichia coli. The significant content of biologically active substances in the lipophilic volatile fraction of Anemone nemorosa indicates the prospects for further study.

Effects of the Tibetan herbal preparation PADMA 28 on blood lipids and lipid oxidisability in subjects with mild hypercholesterolaemia

VASA ◽  
2005 ◽  
Vol 34 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Brunner-La Rocca ◽  
Schindler ◽  
Schlumpf ◽  
Saller ◽  
Suter
Keyword(s):  
Endothelial Dysfunction ◽  
Blood Lipids ◽  
Ex Vivo ◽  
Hdl Cholesterol ◽  
Blood Lipid ◽  
Tibetan Medicine ◽  
Double Blind ◽  
Intraarterial Infusion ◽  
Padma 28

Background: Previous studies showed an anti-atherosclerotic effect of PADMA 28, an herbal formula based on Tibetan medicine. As the mechanisms of action are not fully understood, we investigated whether PADMA 28 may lower blood lipids and lipid oxidisability, and affect early endothelial dysfunction. Patients and methods: Sixty otherwise healthy subjects with total cholesterol ≥5.2 mmol/l and < 8.0 mmol/l were randomly assigned to placebo or PADMA 28, 3 x 2 capsules daily, for 4 weeks (double-blind). Blood lipids (total, LDL-, and HDL-cholesterol, triglycerides, Apo-lipoprotein A1 and B) and ex vivo lipid oxidisability were measured before and after treatment. In a subset of 24 subjects, endothelial function was assessed using venous occlusion plethysmography with intraarterial infusion of acetylcholine. Isolated LDL and plasma both untreated and pre-treated with PADMA 28 extract were oxidised by the radical generator AAPH. Conjugated diene formation was measured at 245 nm. Results: Blood lipids did not change during the study in both groups. In contrast to previous reports in mild hypercholesterolaemia, no endothelial dysfunction was seen and, consequently, was not influenced by therapy. Ex vivo blood lipid oxidisability was significantly reduced with PADMA 28 (area under curve: 5.29 ± 1.62 to 4.99 ± 1.46, p = 0.01), and remained unchanged in the placebo group (5.33 ± 1.88 to 5.18 ± 1.78, p > 0.1). This effect persisted one week after cessation of medication. In vitro experiments confirmed the prevention of lipid peroxidation in the presence of PADMA 28 extracts. Persistent protection was also seen for LDL isolated from PADMA 28-pretreated blood after being subjected to rigorous purification. Conclusions: This study suggests that the inhibition of blood lipid oxidisability by PADMA 28 may play a role in its anti-atherosclerotic effect.

Antimicrobial activity of withanolides from the leaves of Withania aristata

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
L Araujo ◽  
N Padilla ◽  
GG Llanos ◽  
IL Bazzocchi ◽  
L Moujir
Keyword(s):  
Antimicrobial Activity
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