Human Connective Tissue Growth Factor mRNA Expression of Epiretinal and Subretinal Fibrovascular Membranes: A Report of Three Cases

2002 ◽  
Vol 216 (4) ◽  
pp. 284-291 ◽  
Author(s):  
Peter Meyer ◽  
Kerstin Wunderlich ◽  
Hermann L. Kain ◽  
Christian Prünte ◽  
Josef Flammer
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth

scholarly journals Kinetics of Connective Tissue Growth Factor Expression during Experimental Proliferative Glomerulonephritis

2001 ◽  
Vol 12 (3) ◽  
pp. 472-484
Author(s):  
YASUHIKO ITO ◽  
ROEL GOLDSCHMEDING ◽  
RICHARD J. BENDE ◽  
NIKE CLAESSEN ◽  
M. ANWAR CHAND ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Mesangial Cells ◽  
Tissue Growth ◽  
Proliferative Glomerulonephritis ◽  
Kinetics Of ◽  
Tgf Β1

Abstract. Connective tissue growth factor (CTGF) is a member of the CCN family of immediate early genes, which are involved in cell proliferation, migration, and matrix production. Recently, CTGF was observed to be strongly upregulated in human proliferative and fibrogenic renal disease. By in situ hybridization and reverse transcriptase-PCR, the expression of CTGF was investigated in experimental proliferative glomerulonephritis induced by injection of anti—Thy-1.1 antibody in the rat. CTGF expression in cultured rat mesangial cells and glomerular visceral epithelial cells (GVEC) was studied in response to transforming growth factor β (TGF-β), an essential pathogenetic factor in this model. In normal rat kidneys, only some GVEC expressed CTGF mRNA. In anti—Thy-1.1 nephritis, CTGF mRNA expression was strongly increased in extracapillary and mesangial proliferative lesions and in areas of periglomerular fibrosis. Early glomerular CTGF overexpression in GVEC coincided with a striking upregulation of TGF-β2 and to a lesser extent of TGF-β3. Glomerular CTGF mRNA expression was maximal at day 7, in association with increased TGF-β1 mRNA and protein expression. CTGF mRNA overexpression by parietal epithelial cells preceded the periglomerular appearance of α-smooth muscle actin—positive fibroblasts. In cultured mesangial cells, TGF-β1, -β2, and -β3 transiently increased the CTGF/glyceraldehyde phosphate dehydrogenase mRNA ratio up to threefold versus control at 4 h. In GVEC, upregulation of CTGF mRNA by these TGF-β isoforms was more sustained, being 8- to 16-fold versus control at 24 h. The kinetics of CTGF expression strongly suggest a role in glomerular repair, possibly downstream of TGF-β, in this model of transient renal injury.

scholarly journals Hypoxia Induces Connective Tissue Growth Factor mRNA Expression

2009 ◽  
Vol 24 (Suppl 1) ◽  
pp. S176 ◽  
Author(s):  
Young Ki Lee ◽  
Eun-Ji Kim ◽  
Jung Eun Lee ◽  
Jung Woo Noh ◽  
Yoon-Goo Kim
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth

scholarly journals Expression and regulation of osteopontin and connective tissue growth factor transcripts in rat anterior pituitary

2001 ◽  
Vol 169 (1) ◽  
pp. 87-96 ◽  
Author(s):  
J Ehrchen ◽  
H Heuer ◽  
R Sigmund ◽  
MK Schafer ◽  
K Bauer
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Anterior Pituitary ◽  
Tissue Growth ◽  
Cell Interactions ◽  
Female Rats ◽  
Transcript Levels ◽  
Cell Cell

Cell-cell interactions are important regulatory elements in anterior pituitary (AP) physiology. As model systems to study pituitary cell-cell interactions, AP cells kept either as monolayers or as organotypic reaggregate cultures were analyzed by differential display PCR. We identified six cDNA fragments (osteopontin (Opn), connective tissue growth factor (CTGF), alpha(v)-integrin, cathepsin H, lysozyme and O-acetyl GD(3) ganglioside synthase) that showed elevated expression in monolayers compared with reaggregate cultures and the AP. The adenohypophyseal mRNA expression of Opn and CTGF, two secreted signaling substances, was studied in more detail. In situ hybridization histochemistry revealed that Opn mRNA expression is restricted to a subpopulation of gonadotropes whereas CTGF hybridization signals could not be ascribed to any known cell type. Opn transcript levels were downregulated in the APs of lactating rats and decreased when rats received s.c. injections of 17beta-estradiol for 5 days. The mRNA expression was higher in male than in female rats and increased after gonadectomy. CTGF transcript levels were higher in male compared with female rats and were increased in pregnant rats and in rats treated for 5 days with triiodothyronine or dexamethasone. These results indicate that Opn and CTGF may be of physiological importance as local communication factors in the AP.

Connective tissue growth factor (CTGF/CCN2) is increased in peritoneal dialysis patients with high peritoneal solute transport rate

2010 ◽  
Vol 298 (3) ◽  
pp. F721-F733 ◽  
Author(s):  
Makoto Mizutani ◽  
Yasuhiko Ito ◽  
Masashi Mizuno ◽  
Hayato Nishimura ◽  
Yasuhiro Suzuki ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Growth Factor ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Mesothelial Cells ◽  
Tissue Growth ◽  
Transport Rate ◽  
Peritoneal Fibrosis ◽  
Ultrafiltration Failure

Peritoneal fibrosis (PF) is an important complication of peritoneal dialysis (PD) therapy that often occurs in association with peritoneal high transport rate and ultrafiltration failure (UFF). To study the possible pathogenic role of connective tissue growth factor (CTGF) in the relationship of PF and UFF, dialysate CTGF contents ( n = 178) and tissue CTGF expression ( n = 61) were investigated by ELISA, real-time PCR, immunohistochemistry, and in situ hybridization. CTGF production with and without TGF-β1stimulation in human peritoneal mesothelial cells (HPMC) from the spent patients' peritoneal dialysate ( n = 32) was studied in vitro. The dialysate-to-plasma ratio for creatinine (D/P Cr) was positively correlated to dialysate CTGF concentration and estimated local peritoneal production of CTGF. CTGF mRNA expression was 11.4-fold higher in peritoneal membranes with UFF than in pre-PD renal failure peritoneum and was correlated with thickness of the peritoneum. CTGF protein and mRNA were detected in mesothelium and in fibroblast-like cells. In cultured HPMC, TGF-β1-induced expression of CTGF mRNA was increased at 12 and 24 h and was correlated with D/P Cr. In contrast, bone morphogenic protein-4 mRNA expression was inversely correlated with D/P Cr. Our results suggest that high peritoneal transport state is associated with fibrosis and increased peritoneal CTGF expression and production by mesothelial cells, which can be stimulated by TGF-β1. Dialysate CTGF concentration could be a biomarker for both peritoneal fibrosis and membrane function. Functional alteration of mesothelial cells may be involved in progression of peritoneal fibrosis in high transport state.

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