The Bovine Papillomavirus Type-1 E5 Oncoprotein

2001 ◽  
pp. 28-43
Author(s):  
D.J. Goldstein ◽  
J.J. Sparkowski
Keyword(s):  
Bovine Papillomavirus ◽  
E5 Oncoprotein

scholarly journals Inoculation of young horses with bovine papillomavirus type 1 virions leads to early infection of PBMCs prior to pseudo-sarcoid formation

2011 ◽  
Vol 92 (10) ◽  
pp. 2437-2445 ◽  
Author(s):  
Bettina Hartl ◽  
Edmund K. Hainisch ◽  
Saeed Shafti-Keramat ◽  
Reinhard Kirnbauer ◽  
Annunziata Corteggio ◽  
...  
Keyword(s):  
Neutralization Assay ◽  
Bovine Papillomavirus ◽  
Serum Antibodies ◽  
Intact Skin ◽  
Papillomavirus Infection ◽  
E5 Protein ◽  
E5 Oncoprotein ◽  
Young Horses ◽  
And Control

Bovine papillomavirus types 1 and 2 (BPV-1 and BPV-2) are known to induce common equine skin tumours, termed sarcoids. Recently, it was demonstrated that vaccination with BPV-1 virus-like particles (VLPs) is safe and highly immunogenic in horses. To establish a BPV-1 challenge model for evaluation of the protective potential of BPV-1 VLPs, four foals were injected intradermally with infectious BPV-1 virions and with viral genome-based and control inocula, and monitored daily for tumour development. Blood was taken before inoculation and at weekly intervals. BPV-1-specific serum antibodies were detected by a pseudo-virion neutralization assay. Total nucleic acids extracted from tumours, intact skin and PBMCs were tested for the presence of BPV-1 DNA and mRNA using PCR and RT-PCR, respectively. Intralesional E5 oncoprotein expression was determined by immunofluorescence. Pseudo-sarcoids developed exclusively at sites inoculated with virions. Tumours became palpable 11–32 days after virion challenge, reached a size of ≤20 mm in diameter and then resolved in ≤6 months. No neutralizing anti-BPV-1 serum antibodies were detectable pre- or post-challenge. BPV-1 DNA was present in lesions but not in intact skin. In PBMCs, viral DNA was already detectable before lesions were first palpable, in concentrations correlating directly with tumour growth kinetics. PBMCs from two of two foals also harboured E5 mRNA. Immunofluorescence revealed the presence of the E5 protein in tumour fibroblasts, but not in the apparently normal epidermis overlying the lesions. Together with previous findings obtained in horses and cows, these data suggest that papillomavirus infection may include a viraemic phase.

scholarly journals Cooperative Transformation and Coexpression of Bovine Papillomavirus Type 1 E5 and E7 Proteins

2001 ◽  
Vol 75 (1) ◽  
pp. 513-521 ◽  
Author(s):  
Joanna Bohl ◽  
Bruce Hull ◽  
Scott B. Vande Pol
Keyword(s):  
Epithelial Cells ◽  
Cellular Response ◽  
Ectopic Expression ◽  
Granular Cell ◽  
Bovine Papillomavirus ◽  
Basal Cells ◽  
Cultured Epithelial Cells ◽  
E5 Oncoprotein ◽  
E7 Proteins

ABSTRACT Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the basal and spinous layers but not in the granular cell layer. Ectopic expression of E7 in cultured epithelial cells gave rise to localization similar to that seen in productive fibropapillomas, with cytoplasmic and nucleolar expression observed. Consistent with the coexpression of E7 and E5 in basal keratinocytes, BPV-1 E7 cooperated with E5 as well as E6 in an anchorage independence transformation assay. While E5 is expressed in both basal and superficial differentiating keratinocytes, BPV-1 E7 is only observed in basal and lower spinous epithelial cells. Therefore, BPV-1 E7 may serve to modulate the cellular response of basal epithelial cells to E5 expression.

scholarly journals BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals

2013 ◽  
Vol 44 (1) ◽  
pp. 61 ◽  
Author(s):  
Roberta Cotugno ◽  
Dario Gallotta ◽  
Morena d’Avenia ◽  
Annunziata Corteggio ◽  
Gennaro Altamura ◽  
...  
Keyword(s):  
Bovine Papillomavirus ◽  
Death Signals

Efficiency of delivery of DNA to cells by bovine papillomavirus type-1 L1/L2 pseudovirions

2001 ◽  
Vol 56 (1-2) ◽  
pp. 150-156 ◽  
Author(s):  
Y. Liu ◽  
I. H. Frazer ◽  
W. J. Liu ◽  
X. S. Liu ◽  
N. McMillan ◽  
...  
Keyword(s):  
Bovine Papillomavirus
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