Efficiency of delivery of DNA to cells by bovine papillomavirus type-1 L1/L2 pseudovirions

2001 ◽  
Vol 56 (1-2) ◽  
pp. 150-156 ◽  
Author(s):  
Y. Liu ◽  
I. H. Frazer ◽  
W. J. Liu ◽  
X. S. Liu ◽  
N. McMillan ◽  
...  
Keyword(s):  
Bovine Papillomavirus

scholarly journals BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals

2013 ◽  
Vol 44 (1) ◽  
pp. 61 ◽  
Author(s):  
Roberta Cotugno ◽  
Dario Gallotta ◽  
Morena d’Avenia ◽  
Annunziata Corteggio ◽  
Gennaro Altamura ◽  
...  
Keyword(s):  
Bovine Papillomavirus ◽  
Death Signals

scholarly journals Bovine papillomavirus type 1 oncoprotein E5 inhibits equine MHC class I and interacts with equine MHC I heavy chain

2009 ◽  
Vol 90 (12) ◽  
pp. 2865-2870 ◽  
Author(s):  
Barbara Marchetti ◽  
Elisabeth A. Gault ◽  
Marc S. Cortese ◽  
ZhengQiang Yuan ◽  
Shirley A. Ellis ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Class I ◽  
Bovine Papillomavirus ◽  
Class I Mhc ◽  
Mhc I ◽  
Reading Frame ◽  
Histocompatibility Complex ◽  
Human Telomerase

Bovine papillomavirus type 1 is one of the aetiological agents of equine sarcoids. The viral major oncoprotein E5 is expressed in virtually all sarcoids, sarcoid cell lines and in vitro-transformed equine fibroblasts. To ascertain whether E5 behaves in equine cells as it does in bovine cells, we introduced the E5 open reading frame into fetal equine fibroblasts (EqPalF). As observed in primary bovine fibroblasts (BoPalF), E5 by itself could not immortalize EqPalF and an immortalizing gene, such as human telomerase (hTERT/hT), was required for the cells to survive selection. The EqPalF-hT-1E5 cells were morphologically transformed, elongated with many pseudopodia and capable of forming foci. Equine major histocompatibility complex class I (MHC I) was inhibited in these cells at least at two levels: transcription of MHC I heavy chain was inhibited and the MHC I complex was retained in the Golgi apparatus and prevented from reaching the cell surface. We conclude that, as in bovine cells and tumours, E5 is a player in the transformation of equine cells and the induction of sarcoids, and a potential major cause of MHC I downregulation and hence poor immune clearance of tumour cells.

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