Growth Hormone Releasing Hormone Expression During Postnatal Development in Growth Hormone-Deficient Ames Dwarf Mice:mRNA in situ Hybridization

1998 ◽  
Vol 68 (3) ◽  
pp. 201-209 ◽  
Author(s):  
David L. Hurley ◽  
Beth E.F. Wee ◽  
Carol J. Phelps
Keyword(s):  
Growth Hormone ◽  
In Situ Hybridization ◽  
Postnatal Development ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Ames Dwarf

Identification of target cells for growth hormone's action in the arcuate nucleus

1995 ◽  
Vol 269 (4) ◽  
pp. E716-E722
Author(s):  
K. A. Burton ◽  
E. B. Kabigting ◽  
R. A. Steiner ◽  
D. K. Clifton
Keyword(s):  
Growth Hormone ◽  
In Situ Hybridization ◽  
Arcuate Nucleus ◽  
Feedback Mechanism ◽  
Target Cells ◽  
Growth Hormone Releasing Hormone ◽  
Short Loop ◽  
Loop Feedback ◽  
The Brain

Growth hormone (GH) participates in the regulation of its own secretion by acting through a short-loop feedback mechanism to regulate the synthesis and secretion of somatostatin (SS) and growth hormone-releasing hormone (GHRH). The mechanism of GH's action in certain peripheral targets involves the induction of c-fos. Similarly, we hypothesized that GH induces the expression of c-fos mRNA in SS and GHRH neurons in the hypothalamus. Using in situ hybridization, we observed a significant induction of c-fos mRNA in the arcuate nucleus of human GH-treated compared with control animals. Contrary to our hypothesis, only 11% of GHRH mRNA-containing and 5% of SS mRNA-containing neurons colabeled for c-fos mRNA. These findings indicate that GH feedback on the hypothalamus includes the induction of c-fos mRNA primarily in neurons other than GHRH and SS in the arcuate nucleus and suggest that these unidentified neurons located in the arcuate nucleus are directly involved in transducing the effects of GH in the brain.

Growth hormone-releasing hormone peptide and mRNA are overexpressed in GH-deficient Ames dwarf mice.

Endocrinology ◽  
1993 ◽  
Vol 133 (6) ◽  
pp. 3034-3037 ◽  
Author(s):  
C J Phelps ◽  
H Dalcik ◽  
H Endo ◽  
F Talamantes ◽  
D L Hurley
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Ames Dwarf Mice ◽  
Ames Dwarf ◽  
Dwarf Mice

Association of pituitary adenoma with neuronal christoma: A TEM study

1994 ◽  
Vol 52 ◽  
pp. 232-233
Author(s):  
Eva Horvath ◽  
Kalman Kovacs ◽  
B. W. Scheithauer ◽  
R. V. Lloyd ◽  
H. S. Smyth
Keyword(s):  
Growth Hormone ◽  
Pituitary Adenoma ◽  
Pituitary Tumor ◽  
Nervous Tissue ◽  
Alternative Explanation ◽  
Growth Hormone Releasing Hormone ◽  
Cell Hyperplasia ◽  
Releasing Hormone ◽  
Secretory Neurons

The association of a pituitary adenoma with nervous tissue consisting of neuron-like cells and neuropil is a rare abnormality. In the majority of cases, the pituitary tumor is a chromophobic adenoma, accompanied by acromegaly. Histology reveals widely variable proportions of endocrine and nervous tissue in alternating or intermingled patterns. The lesion is perceived as a composite one consisting of two histogenetically distinct parts. It has been suggested that the neuronal component, morphologically similar to secretory neurons of the hypothalamus, may initiate adenoma formation by releasing stimulatory substances. Immunoreactivity for growth hormone releasing hormone (GRH) in the neuronal component of some cases supported this view, whereas other findings such as consistent lack of growth hormone (GH) cell hyperplasia in the lesions called for alternative explanation.Fifteen tumors consisting of a pituitary adenoma and a neuronal component have been collected over a 20 yr. period. Acromegaly was present in 11 patients, was equivocal in one, and absent in 3.

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