Effects of n–3 Polyunsaturated Fatty Acids and Vitamin E on Colonic Mucosal Leukotriene Generation, Lipid Peroxidation, and Microcirculation in Rats with Experimental Colitis

Digestion ◽  
2001 ◽  
Vol 63 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Toshiaki Shimizu ◽  
Jun Igarashi ◽  
Yoshikzu Ohtuka ◽  
Satoshi Oguchi ◽  
Kazunari Kaneko ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Vitamin E ◽  
Polyunsaturated Fatty Acids ◽  
Experimental Colitis

262 EFFECT OF SYSTEMIC ANTIOXIDANT TREATMENT IN BOS TAURUS TAURUS BULLS UNDER HEAT STRESS AND SUPPLEMENTED WITH POLYUNSATURATED FATTY ACIDS

2015 ◽  
Vol 27 (1) ◽  
pp. 220
Author(s):  
E. Gualtieri de Andrade Perez ◽  
J. Diego di Agostini Losano ◽  
A. Dalmazzo ◽  
M. Nichi ◽  
V. Hyppolito Barnabe
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Heat Stress ◽  
Vitamin E ◽  
Polyunsaturated Fatty Acids ◽  
Bos Taurus ◽  
Antioxidant Treatment ◽  
Acute Heat Stress

One reason for lower fertility of European bulls in tropical regions is a higher rate of oxidative stress caused by increased production of reactive oxygen species (ROS) not compensated by antioxidant protection. In that regard, sperm are extremely susceptible to oxidative stress due to a high concentration of polyunsaturated fatty acids (PUFA) in their plasma membranes. However, the presence of these PUFA is fundamental for sperm to be fertile and resistant to cold shock. Thus, treatments that suppress oxidation may increase productivity of these animals. This study aimed to evaluate the most damaging ROS for European bulls subjected to heat stress and to determine a possible antioxidant-targeted treatment. In a second step, we sought to verify the efficiency of the interaction between a diet rich in PUFA and a targeted antioxidant treatment on the quality of ejaculated and epididymal sperm in European bulls subjected to testicular heat stress. Four Bos taurus bulls were subjected to scrotal insulation for 5 days, with semen collection (electroejaculation) 60 days after insulation. Semen from each bull was divided into 4 aliquots and subjected to 4 ROS-generating systems: superoxide anion (xanthine/xanthine oxidase), hydrogen peroxide, hydroxyl radical (ascorbate + ferrous sulfate), and malondialdehyde (MDA; lipid peroxidation product). Samples were incubated for 1 h and assessed by computerized sperm analysis (CASA); eosin/nigrosin (membrane integrity); fast-green/Bengal rose (acrosome integrity); 3,3′ diaminobenzidine (mitochondrial activity); sperm chromatin structure assay (DNA fragmentation); and thiobarbituric acid reactive substances (lipid peroxidation). Overall, MDA had the most deleterious effects on semen quality of Bos taurus bulls subjected to acute heat stress. Thereafter, 16 bulls were subjected to testicular insulation and allocated into 4 groups: control (n = 4; given mineral oil; placebo); vitamin E (n = 4, given 5 mL of Monovin® every 13 days); PUFA (n = 4; given 4 kg day–1 Megalac®); and PUFA+vitamin E (n = 4; combination of PUFA and vitamin E treatment groups). Semen was collected on the day of installation of the insulation, on the day it was removed, and 30 and 60 days later. Overall, vitamin E reduced heat stress-induced damage to sperm DNA and mitochondria, but only in samples collected from the epididymis. Similarly, the combination of vitamin E and PUFA supplementation improved sperm motility patterns. Therefore, a combined antioxidant treatment (vitamin E and PUFA) may reduce damage to sperm caused by acute heat stress in European bulls. However, this treatment may be more effective if instituted before heat stress.

Vitamin E Content and Estimated Need in German Infant and Follow-On Formulas With and Without Long-Chain Polyunsaturated Fatty Acids (LC-PUFA) Enrichment

2014 ◽  
Vol 62 (41) ◽  
pp. 10153-10161 ◽  
Author(s):  
Madlen Stimming ◽  
Christina M. Mesch ◽  
Mathilde Kersting ◽  
Hermann Kalhoff ◽  
Hans Demmelmair ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Polyunsaturated Fatty Acids ◽  
Long Chain

scholarly journals Effect of polyunsaturated fatty acids and antioxidants on lipid peroxidation in tissue cultures.

1981 ◽  
Vol 22 (5) ◽  
pp. 763-769
Author(s):  
V C Gavino ◽  
J S Miller ◽  
S O Ikharebha ◽  
G E Milo ◽  
D G Cornwell
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Tissue Cultures

scholarly journals Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3089
Author(s):  
Lukas M. Müller-Wirtz ◽  
Daniel Kiefer ◽  
Sven Ruffing ◽  
Timo Brausch ◽  
Tobias Hüppe ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Breathing Circuit ◽  
Aliphatic Aldehydes ◽  
Mechanically Ventilated ◽  
Non Invasive ◽  
Plastic Parts ◽  
Ventilated Patients

Exhaled aliphatic aldehydes were proposed as non-invasive biomarkers to detect increased lipid peroxidation in various diseases. As a prelude to clinical application of the multicapillary column–ion mobility spectrometry for the evaluation of aldehyde exhalation, we, therefore: (1) identified the most abundant volatile aliphatic aldehydes originating from in vitro oxidation of various polyunsaturated fatty acids; (2) evaluated emittance of aldehydes from plastic parts of the breathing circuit; (3) conducted a pilot study for in vivo quantification of exhaled aldehydes in mechanically ventilated patients. Pentanal, hexanal, heptanal, and nonanal were quantifiable in the headspace of oxidizing polyunsaturated fatty acids, with pentanal and hexanal predominating. Plastic parts of the breathing circuit emitted hexanal, octanal, nonanal, and decanal, whereby nonanal and decanal were ubiquitous and pentanal or heptanal not being detected. Only pentanal was quantifiable in breath of mechanically ventilated surgical patients with a mean exhaled concentration of 13 ± 5 ppb. An explorative analysis suggested that pentanal exhalation is associated with mechanical power—a measure for the invasiveness of mechanical ventilation. In conclusion, exhaled pentanal is a promising non-invasive biomarker for lipid peroxidation inducing pathologies, and should be evaluated in future clinical studies, particularly for detection of lung injury.

Thiyl radical attack on polyunsaturated fatty acids: A possible route to lipid peroxidation

1989 ◽  
Vol 161 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Christian Schöneich ◽  
Klaus-Dieter Asmus ◽  
Uwe Dillinger ◽  
Franz v. Bruchhausen
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Thiyl Radical ◽  
Radical Attack

scholarly journals Electronic and Tobacco Cigarettes Alter Polyunsaturated Fatty Acids and Oxidative Biomarkers

2021 ◽  
Author(s):  
Rajat Gupta ◽  
Yan Lin ◽  
Karla Luna ◽  
Anjali Logue ◽  
Alexander J Yoon ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Cardiovascular Risk ◽  
Polyunsaturated Fatty Acids ◽  
Low Density Lipoprotein ◽  
Plasma Concentrations ◽  
Multivariable Analysis ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Heme Oxygenase 1

Rationale: Chronic electronic cigarette (EC) users exhibit a higher susceptibility of low-density lipoprotein (LDL) to undergo oxidation as compared to non-user controls. However, there is a paucity of data regarding EC effects on lipid peroxidation in the blood and their relationship to cardiovascular risk. Objective: To test the hypothesis that chronic (≥1 year) EC use exerts intermediate effects on plasma lipid peroxidation and/or antioxidant defense compared to chronic tobacco cigarette (TC) smoking. Methods and Results: We enrolled EC-users (n=32), TC-smokers (n=29) and non-users (n=45), with mean ages of 28.3, 27.8 and 27.4 years, respectively. Plasma concentrations of free polyunsaturated fatty acids and oxidized metabolites were assessed by mass spectrometry. Total antioxidant capacity (TAC), concentrations of glutathione, bilirubin, heme oxygenase-1 (HO-1), and functional activity of paraoxonase1 (PON1) were determined by colorimetric and enzymatic assays. Multivariable analysis was performed using classification models for segregating participants based on biomarker profiles. Plasma arachidonic acid (AA) concentration was higher in TC-smokers but lower in EC-users, together with linoleic acid (LA) concentration, as compared to TC-smokers and non-users (p<0.05). Oxidized LA metabolites (9- and 13-hydroxyoctadecadienoic acid (HODE)) were lower in EC-users and TC-smokers as compared to non-users (p<0.001). Consistently, TAC and bilirubin were elevated in EC-users and TC-smokers as compared to non-users (p<0.05). Of interest, plasma HO-1 concentration was higher in TC-smokers as compared to non-users (p=0.01) with intermediate levels in EC-users. Multivariable analysis identified 5 biomarkers (13-HODE, LA, 9-HODE, 12-hydroxyeicosatetraenoic acid (HETE), AA) that discriminated EC-users from TC-smokers and non-users with an accuracy of 73.4%. Conclusions: Chronic use of EC induces common (i.e. lower 9- and/or 13-HODEs and higher TAC and bilirubin) as well as differential effects (i.e. altered AA and LA concentrations) to those induced by TC, along with intermediate plasma HO-1 concentration, suggesting that EC, likewise TC smoke, could impact cardiovascular risk.

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