Tissue-Specific Expression of Human Angiotensin II AT1 and AT2 Receptors and Cellular Localization of Subtype mRNAs in Adult Human Renal Cortex Using in situ Hybridization

Nephron ◽  
1998 ◽  
Vol 80 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Hiroaki Matsubara ◽  
Takeshi Sugaya ◽  
Satoshi Murasawa ◽  
Yoshihisa Nozawa ◽  
Yasukiyo Mori ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Angiotensin Ii ◽  
Cellular Localization ◽  
Renal Cortex ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Adult Human ◽  
At2 Receptors

Tissue-specific expression of PPAR mRNAs in diabetic rats and divergent effects of cilostazol

2008 ◽  
Vol 86 (7) ◽  
pp. 465-471 ◽  
Author(s):  
Furong Wang ◽  
Ling Gao ◽  
Bendi Gong ◽  
Jianting Hu ◽  
Mei Li ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Renal Cortex ◽  
Expression Patterns ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Peroxisome Proliferator ◽  
Specific Expression ◽  
Camp Content ◽  
Tissue Specific ◽  
Tissue Specific Expression

Cilostazol and ligands of peroxisome proliferator-activated receptors (PPARs) have been effectively used to alleviate diabetic complications, but the common and tissue-specific expression patterns of PPARs in different tissues in diabetic patients and those treated with cilostazol have not been reported. Here, we aimed to assess the effects of diabetes and cilostazol on mRNA expression of PPARα and PPARγ in the aorta, renal cortex, and retina of diabetic rats treated with cilostazol for 8 weeks. PPARα mRNA expression showed uniform downregulation in all these tissues in diabetic rats, and this effect was reversed by cilostazol treatment. Surprisingly, PPARγ mRNA expression was reduced in the renal cortex and retina, yet increased in the aorta of diabetic rats, although cilostazol still reversed these changes. Interestingly, cilostazol, a well-known phosphodiesterase 3 inhibitor and cAMP elevator, augmented cAMP content only in the aorta, but showed no significant effects in the renal cortex of diabetic rats. In conclusion, mRNA expression of PPARs is tissue-specific in diabetes and may be differently affected by cilostazol, possibly because of its tissue-specific effects on cAMP content.

Tandem duplication of the fabp1b gene and subsequent divergence of the tissue-specific distribution of fabp1b.1 and fabp1b.2 transcripts in zebrafish (Danio rerio)

Genome ◽  
2009 ◽  
Vol 52 (12) ◽  
pp. 985-992 ◽  
Author(s):  
Santhosh Karanth ◽  
Eileen M. Denovan-Wright ◽  
Christine Thisse ◽  
Bernard Thisse ◽  
Jonathan M. Wright
Keyword(s):  
In Situ Hybridization ◽  
Linkage Group ◽  
Tandem Duplication ◽  
Duplication Event ◽  
Rt Pcr ◽  
Specific Expression ◽  
Fatty Acid Binding ◽  
Tissue Specific ◽  
Specific Distribution

We describe a fatty acid-binding protein 1 (fabp1b.2) gene and its tissue-specific expression in zebrafish embryos and adults. The 3.5 kb zebrafish fabp1b.2 gene is the paralog of the previously described zebrafish fabp1a and fabp1b genes. Using the LN54 radiation hybrid mapping panel, we assigned the zebrafish fabp1b.2 gene to linkage group 8, the same linkage group to which fabp1b.1 was mapped. fabp1b.1 and fabp1b.2 appear to have arisen by a tandem duplication event. Whole-mount in situ hybridization of a riboprobe to embryos and larvae detected fabp1b.2 transcripts in the diencephalon and as spots in the periphery of the yolk sac. In adult zebrafish, in situ hybridization revealed fabp1b.2 transcripts in the anterior intestine and skin, and reverse transcription PCR (RT-PCR) detected fabp1b.2 transcripts in the intestine, brain, heart, ovary, skin, and eye. By contrast, fabp1b.1 transcripts were detected by RT-PCR in the liver, intestine, heart, testis, ovary, and gills. The tissue-specific distribution of transcripts for the tandemly duplicated fabp1b.1 and fabp1b.2 genes in adult tissues and during development suggests that the duplicated fabp1b genes of zebrafish have acquired additional functions compared with the ancestral fabp1 gene, i.e., by neofunctionalization. Furthermore, these functions were subsequently divided between fabp1b.1 and fabp1b.2 owing to subfunctionalization.

scholarly journals Tissue-specific expression of two Artemia franciscana sarco/endoplasmic reticulum Ca-ATPase isoforms.

1996 ◽  
Vol 44 (4) ◽  
pp. 321-325 ◽  
Author(s):  
R Escalante ◽  
L Sastre
Keyword(s):  
Endoplasmic Reticulum ◽  
Artemia Franciscana ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Tissue Sections ◽  
Evolutionarily Conserved ◽  
Enzyme Isoforms ◽  
The One

The sarco/endoplasmic reticulum Ca-ATPase (SERCA) gene from Artemia franciscana is transcribed into two mRNAs that code for two different enzyme isoforms. We investigated the tissue-specific expression of each mRNA by in situ hybridization of larval tissue sections. One of the isoforms is expressed in the muscle fibers of the appendages. The other isoform is generally expressed throughout all tissues of the larvae. The tissue distribution of these two isoforms is very similar to the one described for the two homologous isoforms generated from the vertebrate SERCA 2 gene, and shows the evolutionarily conserved nature of their tissue-specific expression.

Cloning, cellular localization, genomic organization, and tissue-specific expression of the TGFβ1-inducible SMAP-5 gene

Gene ◽  
2005 ◽  
Vol 351 ◽  
pp. 119-130 ◽  
Author(s):  
Katrin Stolle ◽  
Michael Schnoor ◽  
Georg Fuellen ◽  
Michael Spitzer ◽  
Thomas Engel ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Genomic Organization ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

Tissue-specific expression patterns of nuclear receptors

2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression
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