Tissue specific expression of the Drosophila Adh gene: a comparison of in situ hybridization and immunocytochemistry

Genetica ◽  
1991 ◽  
Vol 84 (2) ◽  
pp. 95-100 ◽  
Author(s):  
S. M. Anderson ◽  
M. R. Brown ◽  
J. F. McDonald
Keyword(s):  
In Situ Hybridization ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Adh Gene ◽  
Drosophila Adh

Tandem duplication of the fabp1b gene and subsequent divergence of the tissue-specific distribution of fabp1b.1 and fabp1b.2 transcripts in zebrafish (Danio rerio)

Genome ◽  
2009 ◽  
Vol 52 (12) ◽  
pp. 985-992 ◽  
Author(s):  
Santhosh Karanth ◽  
Eileen M. Denovan-Wright ◽  
Christine Thisse ◽  
Bernard Thisse ◽  
Jonathan M. Wright
Keyword(s):  
In Situ Hybridization ◽  
Linkage Group ◽  
Tandem Duplication ◽  
Duplication Event ◽  
Rt Pcr ◽  
Specific Expression ◽  
Fatty Acid Binding ◽  
Tissue Specific ◽  
Specific Distribution

We describe a fatty acid-binding protein 1 (fabp1b.2) gene and its tissue-specific expression in zebrafish embryos and adults. The 3.5 kb zebrafish fabp1b.2 gene is the paralog of the previously described zebrafish fabp1a and fabp1b genes. Using the LN54 radiation hybrid mapping panel, we assigned the zebrafish fabp1b.2 gene to linkage group 8, the same linkage group to which fabp1b.1 was mapped. fabp1b.1 and fabp1b.2 appear to have arisen by a tandem duplication event. Whole-mount in situ hybridization of a riboprobe to embryos and larvae detected fabp1b.2 transcripts in the diencephalon and as spots in the periphery of the yolk sac. In adult zebrafish, in situ hybridization revealed fabp1b.2 transcripts in the anterior intestine and skin, and reverse transcription PCR (RT-PCR) detected fabp1b.2 transcripts in the intestine, brain, heart, ovary, skin, and eye. By contrast, fabp1b.1 transcripts were detected by RT-PCR in the liver, intestine, heart, testis, ovary, and gills. The tissue-specific distribution of transcripts for the tandemly duplicated fabp1b.1 and fabp1b.2 genes in adult tissues and during development suggests that the duplicated fabp1b genes of zebrafish have acquired additional functions compared with the ancestral fabp1 gene, i.e., by neofunctionalization. Furthermore, these functions were subsequently divided between fabp1b.1 and fabp1b.2 owing to subfunctionalization.

scholarly journals Tissue-specific expression of two Artemia franciscana sarco/endoplasmic reticulum Ca-ATPase isoforms.

1996 ◽  
Vol 44 (4) ◽  
pp. 321-325 ◽  
Author(s):  
R Escalante ◽  
L Sastre
Keyword(s):  
Endoplasmic Reticulum ◽  
Artemia Franciscana ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Tissue Sections ◽  
Evolutionarily Conserved ◽  
Enzyme Isoforms ◽  
The One

The sarco/endoplasmic reticulum Ca-ATPase (SERCA) gene from Artemia franciscana is transcribed into two mRNAs that code for two different enzyme isoforms. We investigated the tissue-specific expression of each mRNA by in situ hybridization of larval tissue sections. One of the isoforms is expressed in the muscle fibers of the appendages. The other isoform is generally expressed throughout all tissues of the larvae. The tissue distribution of these two isoforms is very similar to the one described for the two homologous isoforms generated from the vertebrate SERCA 2 gene, and shows the evolutionarily conserved nature of their tissue-specific expression.

The in situ localization of Adh transcripts in Drosophila species reveals evolved regulatory differences in spatially restricted expression

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 984-991
Author(s):  
Gogineni Ranganayakulu
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Transcriptional Activity ◽  
Fat Body ◽  
Interspecific Variation ◽  
Specific Expression ◽  
Temporal Aspects ◽  
Drosophila Adh

Spatial and temporal aspects of Adh expression were examined during oogenesis and embryogenesis of Drosophila melanogaster, D. simulans, and D. virilis by in situ hybridization. In stage 14 and 15 embryos, differences in zygotic expression of Adh in the primordia of the gastric caecae of D. simulans and in the fat body of D. virilis were observed. These zygotic differences appear to be transient because Adh expression is seen in the gastric caecae of stage 16 embryos of D. simulans and in the fat body of stage 17 embryos of D. virilis. Analysis of D. melanogaster × D. simulans hybrids revealed that the parental difference for transcriptional activity of Adh in the primordia of the gastric caecae is under dominant control. These results provide the basis for exploring evolved regulatory differences in Adh expression during oogenesis and embryogenesis of Drosophila, which are until now unexplored. The potential of in situ hybridization in analyzing evolved regulatory differences in gene expression is briefly discussed.Key words: Drosophila, Adh, tissue-specific expression, interspecific variation, in situ hybridization.

Tissue-specific expression patterns of nuclear receptors

2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression
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