Pharmacological Analysis of Neurogenic, Sympathetic Responses Mediated through Alpha-1-, Alpha-2-Adrenergic and Purinergic Receptors in the Dog Saphenous Vein

Pharmacology ◽  
2000 ◽  
Vol 60 (4) ◽  
pp. 188-194 ◽  
Author(s):  
Yasuko Hiraoka ◽  
Takanobu Taniguchi ◽  
Masafumi Oshita ◽  
Ikunobu Muramatsu
Keyword(s):  
Saphenous Vein ◽  
Purinergic Receptors ◽  
Dog Saphenous Vein ◽  
Pharmacological Analysis ◽  
Sympathetic Responses

Relationship between dog saphenous vein reactivity and Na content

1988 ◽  
Vol 255 (4) ◽  
pp. H860-H865
Author(s):  
V. Berczi ◽  
G. Simon
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Saphenous Vein ◽  
Dna Content ◽  
Mode Of Action ◽  
Venous Blood ◽  
Dog Saphenous Vein ◽  
Wide Range ◽  
Venous Wall ◽  
Stimulation Of

The physiological significance of the wide range of spontaneous variation in the total Na content of the dog saphenous vein (SV) was investigated. The SV of pentobarbital-anesthetized male mongrel dogs was perfused in vitro with the dogs' own venous blood, and its reactivity to acetylcholine (ACh) and norepinephrine (NE) was measured. The contralateral SV was removed for measurements of total and intracellular (Li exchange at 4 degrees C) Na and K content, DNA content, and muscle width. Reactivity to ACh correlated directly with total and extracellular SV Na content, and reactivity to NE correlated directly with total and intracellular K content. Reactivity to NE was unrelated to ACh reactivity, plasma NE concentration, or venous wall DNA content or muscle width. ACh-mediated venoconstriction was approximately 10 times more sensitive to inhibition by amiloride, an inhibitor of Na-entry pathways, than NE-mediated venoconstriction. The finding that extracellular Na content is a marker of reactivity to ACh is compatible with experimental evidence that the mode of action of ACh may be the stimulation of Na influx. The positive correlation between the K content and reactivity of veins to NE suggests that there is a link between intracellular K content and the release of Ca from the sarcoplasmic reticulum in response to NE.

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