Effect of Spleen-Cell-Conditioned Medium on [3H]-Choline Uptake by Retinal Cells in vitro Is Mediated by IL-2

2000 ◽  
Vol 7 (4) ◽  
pp. 195-207 ◽  
Author(s):  
Alfred Sholl-Franco ◽  
Patrícia M.B. Marques ◽  
Roberto Paes-de-Carvalho ◽  
Elizabeth G. de Araujo
Keyword(s):  
Spleen Cell ◽  
Conditioned Medium ◽  
Choline Uptake ◽  
Retinal Cells ◽  
Cell Conditioned Medium

Presence of multipotential hemopoietic cells in teratocarcinoma cultures

Development ◽  
1981 ◽  
Vol 61 (1) ◽  
pp. 51-59
Author(s):  
Claude A. Cudennec ◽  
Gregory R. Johnson
Keyword(s):  
Red Blood Cells ◽  
Spleen Cell ◽  
Conditioned Medium ◽  
Cell Population ◽  
Mouse Embryo ◽  
Blood Cells ◽  
Pokeweed Mitogen ◽  
Hemopoietic Cell ◽  
Cell Conditioned Medium

Previous investigations have shown that the teratocarcinoma line PCC3/A/1 is able to differentiate in vitro to produce red blood cells of the primitive hemopoietic cell population of the mouse embryo. The present paper demonstrates the presence in the same cultures of multipotential cells capable of giving rise to colonies containing erythrocytes, macrophages, and megakaryocytes when stimulated by pokeweed-mitogen spleen-cell conditioned medium.

scholarly journals Effects of sciatic-conditioned medium on neonatal rat retinal cells in vitro

1998 ◽  
Vol 31 (11) ◽  
pp. 1439-1442 ◽  
Author(s):  
P.M.M. Torres ◽  
C.V.V. Guilarducci ◽  
E.G. Araujo
Keyword(s):  
Conditioned Medium ◽  
Neonatal Rat ◽  
Retinal Cells

scholarly journals Bisphenol A Altersβ-hCG and MIF Release by Human Placenta: AnIn VitroStudy to Understand the Role of Endometrial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
C. Mannelli ◽  
F. Ietta ◽  
C. Carotenuto ◽  
R. Romagnoli ◽  
A. Z. Szostek ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Conditioned Medium ◽  
Human Placenta ◽  
Environmental Chemicals ◽  
Endometrial Cells ◽  
Direct Exposure ◽  
And Control ◽  
Cell Conditioned Medium

A proper fetomaternal immune-endocrine cross-talk in pregnancy is fundamental for reproductive success. This might be unbalanced by exposure to environmental chemicals, such as bisphenol A (BPA). As fetoplacental contamination with BPA originates from the maternal compartment, this study investigated the role of the endometrium in BPA effects on the placenta. To this end,in vitrodecidualized stromal cells were exposed to BPA 1 nM, and their conditioned medium (diluted 1 : 2) was used on chorionic villous explants from human placenta. Parallel cultures of placental explants were directly exposed to 0.5 nM BPA while, control cultures were exposed to the vehicle (EtOH 0.1%). After 24–48 h, culture medium from BPA-treated and control cultures was assayed for concentration of hormone human Chorionic Gonadotropin (β-hCG) and cytokine Macrophage Migration Inhibitory Factor (MIF). The results showed that direct exposure to BPA stimulated the release of both MIF andβ-hCG. These effects were abolished/diminished in placental cultures exposed to endometrial cell-conditioned medium. GM-MS analysis revealed that endometrial cells retain BPA, thus reducing the availability of this chemical for the placenta. The data obtained highlight the importance ofin vitromodels including the maternal component in reproducing the effects of environmental chemicals on human fetus/placenta.

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

scholarly journals Inhibition against CFU-C and CFU-E colony formation by soluble factor(s) derived from hairy cells

Blood ◽  
1989 ◽  
Vol 73 (4) ◽  
pp. 907-913 ◽  
Author(s):  
N Taniguchi ◽  
H Kuratsune ◽  
A Kanamaru ◽  
Y Tokumine ◽  
S Tagawa ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Colony Formation ◽  
Inhibitory Effect ◽  
Lymphocytic Leukemia ◽  
Hairy Cell ◽  
Cell Leukemia ◽  
Hairy Cells ◽  
Cell Conditioned Medium ◽  
Normal Controls

Abstract The inhibitory effect by hairy cell conditioned medium (HCCM) on the growth of granulocyte and erythrocyte colony forming cells was studied in vitro. The percent inhibition of CFU-C formation by HCCM from four hairy cell leukemia (HCL) patients ranged from 36% to 76%, while no inhibition was observed with conditioned medium (CM) obtained from three B-cell chronic lymphocytic leukemia (B-CLL) patients nor from two normal controls. HCCM inhibited specially the growth of rG-CSF responding stem cells. The hairy cell-derived colony inhibitory factor from HCCM was nondialyzable, fairly stable to heat treatment, and trypsin sensitive. Its maximal inhibitory activity against granulopoiesis was observed in the fractions of 5,000 to 6,000 daltons. Moreover HCCM inhibited CFU-E colony formation but not BFU-E. These results indicate that hairy cells produce a factor that inhibits granulopoiesis and erythropoiesis in vitro. This factor may play a role in neutropenia and anemia observed in HCL.

Megakaryocyte Colony-Stimulating Factor and Burst-Promoting Activity in LPS-Treated Mouse Spleen Cell-Conditioned Medium

Pathobiology ◽  
1988 ◽  
Vol 56 (5) ◽  
pp. 245-253
Author(s):  
Masakuni Sugimoto ◽  
Yoshihisa Wakabayashi ◽  
Shun-ichi Hirose ◽  
Martin J. Murphy, Jr
Keyword(s):  
Spleen Cell ◽  
Conditioned Medium ◽  
Treated Mouse ◽  
Mouse Spleen ◽  
Colony Stimulating Factor ◽  
Mouse Spleen Cell ◽  
Stimulating Factor ◽  
Cell Conditioned Medium
Sign in / Sign up

Export Citation Format

Share Document