Megakaryocyte Colony-Stimulating Factor and Burst-Promoting Activity in LPS-Treated Mouse Spleen Cell-Conditioned Medium

Pathobiology ◽  
1988 ◽  
Vol 56 (5) ◽  
pp. 245-253
Author(s):  
Masakuni Sugimoto ◽  
Yoshihisa Wakabayashi ◽  
Shun-ichi Hirose ◽  
Martin J. Murphy, Jr
Keyword(s):  
Spleen Cell ◽  
Conditioned Medium ◽  
Treated Mouse ◽  
Mouse Spleen ◽  
Colony Stimulating Factor ◽  
Mouse Spleen Cell ◽  
Stimulating Factor ◽  
Cell Conditioned Medium

scholarly journals Selective stimulation by mouse spleen cell conditioned medium of human eosinophil colony formation

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 999-1005
Author(s):  
D Metcalf ◽  
RL Cutler ◽  
NA Nicola
Keyword(s):  
Spleen Cell ◽  
Conditioned Medium ◽  
Colony Formation ◽  
Culture System ◽  
Mouse Spleen ◽  
Mouse Spleen Cell ◽  
Human Eosinophil ◽  
Eosinophil Colonies ◽  
Cell Conditioned Medium ◽  
Marrow Cells

Stimulation of unfractionated or nonadherent human marrow cells in agar culture by pokeweed-mitogen-stimulated BALB/c mouse spleen cell conditioned medium (SCM) led, in most cultures, to the exclusive formation of eosinophil colonies. The culture system exhibited linearity of eosinophil colony formation with varying numbers of cells cultured, and the absolute numbers and size of SCM-stimulated eosinophil colonies approximated those in cultures stimulated by human placental conditioned medium. The active factor in SCM for human eosinophil colony formation was not clearly separable from the factors stimulating granulocyte-macrophage and eosinophil colony formation by mouse marrow cells on ammonium sulfate and phenyl boronate chromatography, but was of larger size than the mouse-active factors and separable from them by phenyl sepharose chromatography. This selective culture system for eosinophil colony formation should be of value for studies on human eosinophil progenitor and maturing cell populations in a variety of disease states.

scholarly journals Selective stimulation by mouse spleen cell conditioned medium of human eosinophil colony formation

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 999-1005 ◽  
Author(s):  
D Metcalf ◽  
RL Cutler ◽  
NA Nicola
Keyword(s):  
Spleen Cell ◽  
Conditioned Medium ◽  
Colony Formation ◽  
Culture System ◽  
Mouse Spleen ◽  
Mouse Spleen Cell ◽  
Human Eosinophil ◽  
Eosinophil Colonies ◽  
Cell Conditioned Medium ◽  
Marrow Cells

Abstract Stimulation of unfractionated or nonadherent human marrow cells in agar culture by pokeweed-mitogen-stimulated BALB/c mouse spleen cell conditioned medium (SCM) led, in most cultures, to the exclusive formation of eosinophil colonies. The culture system exhibited linearity of eosinophil colony formation with varying numbers of cells cultured, and the absolute numbers and size of SCM-stimulated eosinophil colonies approximated those in cultures stimulated by human placental conditioned medium. The active factor in SCM for human eosinophil colony formation was not clearly separable from the factors stimulating granulocyte-macrophage and eosinophil colony formation by mouse marrow cells on ammonium sulfate and phenyl boronate chromatography, but was of larger size than the mouse-active factors and separable from them by phenyl sepharose chromatography. This selective culture system for eosinophil colony formation should be of value for studies on human eosinophil progenitor and maturing cell populations in a variety of disease states.

scholarly journals Eosinophil activation by colony-stimulating factor in man: metabolic effects and analysis by flow cytometry

Blood ◽  
1983 ◽  
Vol 61 (6) ◽  
pp. 1232-1241
Author(s):  
MA Vadas ◽  
G Varigos ◽  
N Nicola ◽  
S Pincus ◽  
A Dessein ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Protein Synthesis ◽  
Conditioned Medium ◽  
Superoxide Production ◽  
Metabolic Effects ◽  
Mouse Spleen ◽  
Mouse Lung ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

Substantial increases in the killing capacity of human eosinophils after in vitro incubation with human placental conditioned medium (HPCM), a standard source of colony-stimulating factor (CSF), have recently been described. In this article, the interaction between HPCM and purified human eosinophils is analyzed by flow cytometry and by effects on iodination, superoxide production, and protein synthesis. HPCM increased the intensity of natural eosinophil autofluorescence (aFlu) (460 nm) after the absorption of ultraviolet light (360 nm) in a manner that was both time and dose dependent. Measured in arbitrary units, eosinophil aFlu was 72 +/- 7.3 (arithmetic mean +/- SEM) and 121 +/- 3.2 after 18-hr incubations in the absence or presence of HPCM, respectively. The activity in HPCM responsible for these changes cochromatographed on Ultrogel AcA44 columns with CSF and with the less hydrophobic variant of CSF (CSF-alpha) on phenyl Sepharose. Mouse spleen, but not mouse lung, conditioned medium was also active on human eosinophils in this assay. Both CSF-alpha and mouse spleen conditioned medium also contain eosinophil colony-stimulating activity (CSA), whereas inactive CSFs with no effect on mature eosinophils, CSF-beta, and mouse lung conditioned medium also lack eosinophil CSA. CSF-alpha stimulated superoxide production of resting eosinophils (from 0.03 +/- 0.03 to 0.47 +/- 0.08 nmole cytochrome-c reduced/10(5) eosinophils) and of eosinophils incubated with preopsonized zymosan (from 0.15 +/- 0.06 to 0.73 +/- 0.07). It also stimulated iodination by resting eosinophils (from 0.76 +/- 0.16 to 2.60 +/- 0.72 nmoles l/10(7) eosinophils/hr) and of eosinophils incubated with preopsonized zymosan (from 7.52 +/- 2.08 to 29.8 +/- 1.32). In contrast, CSF-beta was inactive in these assays. CSF-alpha also stimulated, between 2- and 15-fold, the new protein synthesis of eosinophils. Thus, substances that stimulate the differentiation of progenitor cells into eosinophils also interact with peripheral mature eosinophils, and the activation of postmitotic cells may be a physiologic role of CSF-like molecules.

scholarly journals Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor.

1976 ◽  
Vol 143 (3) ◽  
pp. 631-647 ◽  
Author(s):  
E R Stanley ◽  
M Cifone ◽  
P M Heard ◽  
V Defendi
Keyword(s):  
Growth Factor ◽  
Conditioned Medium ◽  
Peritoneal Macrophages ◽  
Partial Purification ◽  
Colony Stimulating Factor ◽  
L Cell ◽  
Antigenic Properties ◽  
Cell Medium ◽  
Stimulating Factor ◽  
Cell Conditioned Medium

The activities of a colony-stimulating factor (CSF), which stimulates granulocyte-macrophage colony formation by mouse hemopoietic cells, and macrophage growth factor (MGF), which stimulates proliferation of activated peritoneal macrophages, have been demonstrated by various criteria to reside in the same molecular species. These criteria include occurrence in various sources and copurification of the activities in mouse L-cell-conditioned medium as well as the biological, physicochemical, and antigenic properties of the activities of L-cell-conditioned medium. CSF and MGF activities of L-cell-conditioned medium are ascribable to a glycoprotein of mol wt approximately 60,000 which migrates electrophoretically with alpha-globulin. Human urinary CSF, which also possesses MGF activity, has similar properties and can be neutralized by antiserum to highly purified L-cell medium CSF. A procedure is described for the partial purification of material from L-cell medium that has activity at 1 ng/ml in both MGF and CSF assays.

scholarly journals Purification of two forms of colony-stimulating factor from mouse L-cell-conditioned medium.

1985 ◽  
Vol 260 (29) ◽  
pp. 16004-16011 ◽  
Author(s):  
A W Burgess ◽  
D Metcalf ◽  
I J Kozka ◽  
R J Simpson ◽  
G Vairo ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Colony Stimulating Factor ◽  
L Cell ◽  
Stimulating Factor ◽  
Cell Conditioned Medium

scholarly journals Eosinophil activation by colony-stimulating factor in man: metabolic effects and analysis by flow cytometry

Blood ◽  
1983 ◽  
Vol 61 (6) ◽  
pp. 1232-1241 ◽  
Author(s):  
MA Vadas ◽  
G Varigos ◽  
N Nicola ◽  
S Pincus ◽  
A Dessein ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Protein Synthesis ◽  
Conditioned Medium ◽  
Superoxide Production ◽  
Metabolic Effects ◽  
Mouse Spleen ◽  
Mouse Lung ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

Abstract Substantial increases in the killing capacity of human eosinophils after in vitro incubation with human placental conditioned medium (HPCM), a standard source of colony-stimulating factor (CSF), have recently been described. In this article, the interaction between HPCM and purified human eosinophils is analyzed by flow cytometry and by effects on iodination, superoxide production, and protein synthesis. HPCM increased the intensity of natural eosinophil autofluorescence (aFlu) (460 nm) after the absorption of ultraviolet light (360 nm) in a manner that was both time and dose dependent. Measured in arbitrary units, eosinophil aFlu was 72 +/- 7.3 (arithmetic mean +/- SEM) and 121 +/- 3.2 after 18-hr incubations in the absence or presence of HPCM, respectively. The activity in HPCM responsible for these changes cochromatographed on Ultrogel AcA44 columns with CSF and with the less hydrophobic variant of CSF (CSF-alpha) on phenyl Sepharose. Mouse spleen, but not mouse lung, conditioned medium was also active on human eosinophils in this assay. Both CSF-alpha and mouse spleen conditioned medium also contain eosinophil colony-stimulating activity (CSA), whereas inactive CSFs with no effect on mature eosinophils, CSF-beta, and mouse lung conditioned medium also lack eosinophil CSA. CSF-alpha stimulated superoxide production of resting eosinophils (from 0.03 +/- 0.03 to 0.47 +/- 0.08 nmole cytochrome-c reduced/10(5) eosinophils) and of eosinophils incubated with preopsonized zymosan (from 0.15 +/- 0.06 to 0.73 +/- 0.07). It also stimulated iodination by resting eosinophils (from 0.76 +/- 0.16 to 2.60 +/- 0.72 nmoles l/10(7) eosinophils/hr) and of eosinophils incubated with preopsonized zymosan (from 7.52 +/- 2.08 to 29.8 +/- 1.32). In contrast, CSF-beta was inactive in these assays. CSF-alpha also stimulated, between 2- and 15-fold, the new protein synthesis of eosinophils. Thus, substances that stimulate the differentiation of progenitor cells into eosinophils also interact with peripheral mature eosinophils, and the activation of postmitotic cells may be a physiologic role of CSF-like molecules.

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