Hepatitis A Virus Proteins

Intervirology ◽  
1999 ◽  
Vol 42 (2-3) ◽  
pp. 63-68 ◽  
Author(s):  
Atsuko Totsuka ◽  
Yasuo Moritsugu
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Proteins

scholarly journals Solubility as a limiting factor for expression of hepatitis A virus proteins in insect cell-baculovirus system

2016 ◽  
Vol 111 (8) ◽  
pp. 535-538
Author(s):  
Haroldo Cid da Silva Junior ◽  
Cristiane Pinheiro Pestana ◽  
Ricardo Galler ◽  
Marco Alberto Medeiros
Keyword(s):  
Insect Cell ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Limiting Factor ◽  
Baculovirus System ◽  
Virus Proteins

Prediction of Secondary Structure, Spatial Organization and Distribution of Antigenic Determinants for Hepatitis A Virus Proteins

1987 ◽  
Vol 5 (2) ◽  
pp. 447-458 ◽  
Author(s):  
Vadim V. Mesyanzhinov ◽  
Elena N. Peletskaya ◽  
Viktor M. Zhdanov ◽  
Alexander V. Efimov ◽  
Alexey V. Finkelstein ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Hepatitis A ◽  
Spatial Organization ◽  
Hepatitis A Virus ◽  
Antigenic Determinants ◽  
Prediction Of Secondary Structure ◽  
Virus Proteins

scholarly journals Membrane Permeability Induced by Hepatitis A Virus Proteins 2B and 2BC and Proteolytic Processing of HAV 2BC

Virology ◽  
1998 ◽  
Vol 252 (1) ◽  
pp. 218-227 ◽  
Author(s):  
Monika Jecht ◽  
Christian Probst ◽  
Verena Gauss-Müller
Keyword(s):  
Membrane Permeability ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Proteolytic Processing ◽  
Virus Proteins

Mutational modifications of hepatitis A virus proteins 2B and 2C described for cell culture-adapted and attenuated virus are present in wild-type virus populations

2014 ◽  
Vol 159 (10) ◽  
pp. 2699-2704 ◽  
Author(s):  
Rebecca Weilandt ◽  
Dajana Paulmann ◽  
Kore Schlottau ◽  
Angelika Vallbracht ◽  
Andreas Dotzauer
Keyword(s):  
Cell Culture ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Wild Type Virus ◽  
Type Virus ◽  
Wild Type ◽  
Attenuated Virus ◽  
Virus Proteins

Perinuclear accumulation of hepatitis A virus proteins, RNA, and particles and ultrastructural alterations in infected cells

2001 ◽  
Vol 146 (12) ◽  
pp. 2291-2307 ◽  
Author(s):  
M. H. F. Klinger ◽  
R. Kämmerer ◽  
B. Hornei ◽  
V. Gauss-Müller
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Ultrastructural Alterations ◽  
Infected Cells ◽  
Virus Proteins

Detection of hepatitis A virus proteins in infected BS-C-1 cells

Virology ◽  
1991 ◽  
Vol 185 (1) ◽  
pp. 411-418 ◽  
Author(s):  
Wanda S. Updike ◽  
Michael Tesar ◽  
Ellie Ehrenfeld
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Proteins

Cellular localization and effects of ectopically expressed hepatitis A virus proteins 2B, 2C, 3A and their intermediates 2BC, 3AB and 3ABC

2015 ◽  
Vol 161 (4) ◽  
pp. 851-865 ◽  
Author(s):  
Nicole Seggewiß ◽  
Hedi Verena Kruse ◽  
Rebecca Weilandt ◽  
Erna Domsgen ◽  
Andreas Dotzauer ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Proteins

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Hepatitis A Antigen in Liver, Bile and Stool

1975 ◽  
Vol 33 ◽  
pp. 340-341
Author(s):  
D.R. Jackson ◽  
J.H. Hoofnagle ◽  
A.N. Schulman ◽  
J.L. Dienstag ◽  
R.H. Purcell ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Liver Enzymes ◽  
Hepatitis A Virus ◽  
Type A ◽  
Initial Study ◽  
Virus Like Particle ◽  
Elevated Liver Enzymes ◽  
Ag Particles ◽  
Ag Particle ◽  
Human Stool

Using immune electron microscopy Feinstone et. al. demonstrated the presence of a 27 nm virus-like particle in acute-phase stools of patients with viral hepatitis, type A, These hepatitis A antigen (HA Ag) particles were aggregated by convalescent serum from patients with type A hepatitis but not by pre-infection serum. Subsequently Dienstag et. al. and Maynard et. al. produced acute hepatitis in chimpanzees by inoculation with human stool containing HA Ag. During the early acute disease, virus like particles antigenically, morphologically and biophysically identical to the human HA Ag particle were found in chimpanzee stool. Recently Hilleman et. al. have described similar particles in liver and serum of marmosets infected with hepatitis A virus (HAV). We have investigated liver, bile and stool from chimpanzees and marmosets experimentally infected with HAV. In an initial study, a chimpanzee (no.785) inoculated with HA Ag-containing stool developed elevated liver enzymes 21 days after exposure.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

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