Perinuclear accumulation of hepatitis A virus proteins, RNA, and particles and ultrastructural alterations in infected cells

2001 ◽  
Vol 146 (12) ◽  
pp. 2291-2307 ◽  
Author(s):  
M. H. F. Klinger ◽  
R. Kämmerer ◽  
B. Hornei ◽  
V. Gauss-Müller
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Ultrastructural Alterations ◽  
Infected Cells ◽  
Virus Proteins

scholarly journals Solubility as a limiting factor for expression of hepatitis A virus proteins in insect cell-baculovirus system

2016 ◽  
Vol 111 (8) ◽  
pp. 535-538
Author(s):  
Haroldo Cid da Silva Junior ◽  
Cristiane Pinheiro Pestana ◽  
Ricardo Galler ◽  
Marco Alberto Medeiros
Keyword(s):  
Insect Cell ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Limiting Factor ◽  
Baculovirus System ◽  
Virus Proteins

scholarly journals Heme Oxygenase-1 Exerts Antiviral Activity against Hepatitis A Virus In Vitro

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1229
Author(s):  
Dong-Hwi Kim ◽  
Hee-Seop Ahn ◽  
Hyeon-Jeong Go ◽  
Da-Yoon Kim ◽  
Jae-Hyeong Kim ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Protein Expression ◽  
Heme Oxygenase ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Health Concern ◽  
Heme Oxygenase 1 ◽  
Infected Cells ◽  
In Cells

Hepatitis A virus (HAV), the causative pathogen of hepatitis A, induces severe acute liver injuries in humans and is a serious public health concern worldwide. However, appropriate therapeutics have not yet been developed. The enzyme heme oxygenase-1 (HO-1) exerts antiviral activities in cells infected with several viruses including hepatitis B and C viruses. In this study, we demonstrated for the first time the suppression of virus replication by HO-1 in cells infected with HAV. Hemin (HO-1 inducer) induced HO-1 mRNA and protein expression, as expected, and below 50 mM, dose-dependently reduced the viral RNA and proteins in the HAV-infected cells without cytotoxicity. Additionally, HO-1 protein overexpression using a protein expression vector suppressed HAV replication. Although ZnPP-9, an HO-1 inhibitor, did not affect HAV replication, it significantly inhibited hemin-induced antiviral activity in HAV-infected cells. Additionally, FeCl3, CORM-3, biliverdin, and the HO-1 inducers andrographolide and CoPP inhibited HAV replication in the HAV-infected cells; andrographolide and CoPP exhibited a dose-dependent effect. In conclusion, these results suggest that HO-1 effectively suppresses HAV infection in vitro, and its enzymatic products appear to exert antiviral activity. We expect that these results could contribute to the development of a new antiviral drug for HAV.

scholarly journals Detection of Hepatitis A Virus by Using a Combined Cell Culture-Molecular Beacon Assay

2008 ◽  
Vol 74 (7) ◽  
pp. 2239-2243 ◽  
Author(s):  
Hsiao-Yun Yeh ◽  
Yu-Chen Hwang ◽  
Marylynn V. Yates ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Cell Culture ◽  
Hepatitis A ◽  
Molecular Beacon ◽  
Hepatitis A Virus ◽  
Mammalian Cell Culture ◽  
Fluorescence Signal ◽  
Cytopathic Effects ◽  
Infected Cells ◽  
Fetal Rhesus Monkey Kidney ◽  
Fluorescent Cells

ABSTRACT Rapid and efficient methods for the detection and quantification of infectious viruses are required for public health risk assessment. Current methods to detect infectious viruses are based on mammalian cell culture and rely on the production of visible cytopathic effects (CPE). For hepatitis A virus (HAV), viral replication in cell culture has been reported to be nonlytic and relatively slow. It may take more than 1 week to reach the maximum production and subsequent visualization of CPE. A molecular beacon (MB), H1, specifically targeting a 20-bp 5′ noncoding region of HAV, was designed and synthesized. MB H1 was introduced into fixed and permeabilized fetal rhesus monkey kidney (FRhK-4) cells infected with HAV strain HM-175. Upon hybridizing with the viral mRNA, fluorescent cells were visualized easily under a fluorescence microscope. Discernible fluorescence was detected only in infected cells by using the specific MB H1. A nonspecific MB, which was not complementary to the viral RNA sequence, produced no visible fluorescence signal. This MB-based fluorescence assay enabled the direct counting of fluorescent cells and could achieve a detection limit of 1 PFU at 6 h postinfection, demonstrating a significant improvement in viral quantification over current infectivity assays.

Prediction of Secondary Structure, Spatial Organization and Distribution of Antigenic Determinants for Hepatitis A Virus Proteins

1987 ◽  
Vol 5 (2) ◽  
pp. 447-458 ◽  
Author(s):  
Vadim V. Mesyanzhinov ◽  
Elena N. Peletskaya ◽  
Viktor M. Zhdanov ◽  
Alexander V. Efimov ◽  
Alexey V. Finkelstein ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Hepatitis A ◽  
Spatial Organization ◽  
Hepatitis A Virus ◽  
Antigenic Determinants ◽  
Prediction Of Secondary Structure ◽  
Virus Proteins

scholarly journals CXCL10 is produced in hepatitis A virus-infected cells in an IRF3-dependent but IFN-independent manner

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Pil Soo Sung ◽  
Seon-Hui Hong ◽  
Jeewon Lee ◽  
Su-Hyung Park ◽  
Seung Kew Yoon ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Independent Manner ◽  
Infected Cells

Hepatitis A Virus Proteins

Intervirology ◽  
1999 ◽  
Vol 42 (2-3) ◽  
pp. 63-68 ◽  
Author(s):  
Atsuko Totsuka ◽  
Yasuo Moritsugu
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Proteins

scholarly journals Membrane Permeability Induced by Hepatitis A Virus Proteins 2B and 2BC and Proteolytic Processing of HAV 2BC

Virology ◽  
1998 ◽  
Vol 252 (1) ◽  
pp. 218-227 ◽  
Author(s):  
Monika Jecht ◽  
Christian Probst ◽  
Verena Gauss-Müller
Keyword(s):  
Membrane Permeability ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Proteolytic Processing ◽  
Virus Proteins

Mutational modifications of hepatitis A virus proteins 2B and 2C described for cell culture-adapted and attenuated virus are present in wild-type virus populations

2014 ◽  
Vol 159 (10) ◽  
pp. 2699-2704 ◽  
Author(s):  
Rebecca Weilandt ◽  
Dajana Paulmann ◽  
Kore Schlottau ◽  
Angelika Vallbracht ◽  
Andreas Dotzauer
Keyword(s):  
Cell Culture ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Wild Type Virus ◽  
Type Virus ◽  
Wild Type ◽  
Attenuated Virus ◽  
Virus Proteins
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