Ki-67 Immunostaining of Normal Human Epidermis: Comparison with 3H-Thymidine Labelling and PCNA Immunostaining

Dermatology ◽  
1998 ◽  
Vol 197 (2) ◽  
pp. 123-126 ◽  
Author(s):  
M. Heenen ◽  
S. Thiriar ◽  
J.-C. Noël ◽  
P. Galand
Keyword(s):  
Human Epidermis ◽  
Ki 67 ◽  
Thymidine Labelling ◽  
Normal Human

Collagenolytic-like activity of normal human epidermis

1971 ◽  
Vol 242 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Kays H. Kaidbey ◽  
Amal K. Kurban ◽  
Krikor Tenekjian
Keyword(s):  
Human Epidermis ◽  
Normal Human

Polo-like kinase expression in normal human endometrium during the menstrual cycle

2000 ◽  
Vol 12 (2) ◽  
pp. 59 ◽  
Author(s):  
Noriyuki Takai ◽  
Tami Miyazaki ◽  
Isao Miyakawa ◽  
Ryoji Hamanaka
Keyword(s):  
Menstrual Cycle ◽  
Stromal Cells ◽  
Cellular Proliferation ◽  
Secretory Phase ◽  
Ki 67 ◽  
Normal Endometrium ◽  
Proliferative Phase ◽  
Normal Human ◽  
Late Secretory Phase

The enzyme, polo-like kinase (PLK), is a mammalian serine/threonine kinase involved in cell cycle regulation. A great deal of evidence regarding the role of PLK in the cell cycle has been obtained through studies of cultured cells, though little is known about its function or even expression in vivo. The endometrium undergoes rapid proliferation and differentiation under ovarian steroid hormone control during the 28-day cycle. Thus, normal endometrium provides an excellent model in which to study the hormone dependency of PLK expression. In the present study, we examined the features of PLK expression in 20 samples of normal human endometrium during the menstrual cycle. The expression of Ki-67 and proliferating cell nuclear antigen (PCNA) were also examined as markers of proliferation. Immunohistochemical studies showed that PLK staining was detected in the basement membrane of many endometrial glands, stromal cells, and some endothelial cells. The number of PLK-positive endometrial gland cells was significantly higher in the late proliferative phase (19.16% 4.98%) and the early secretory phase (19.28% 4.99%) than in the early proliferative phase (2.60% 2.33%) or the late secretory phase (5.76% 2.16%) (P<0.0001). PLK expression seemed to be correlated with the expression of Ki-67 and PCNA in many endometrial glands and stromal cells particularly in the late proliferative phase, reflecting a role of PLK in cellular proliferation. Nevertheless, in the early secretory phase, at which point the expression of Ki-67 and PCNA decreased in endometrial glands, PLK was strongly expressed. This finding suggests that PLK may have some post-mitotic functions in certain specialized cell types. Although the highest expression of PLK was observed in the late proliferative and the early secretory phases, the expression drastically decreased in the late secretory phase. These findings, taken together, indicate that the expression of PLK in normal endometrium fluctuates over the course of the menstrual cycle, suggesting in turn that PLK is associated with hormone-dependent cellular proliferation and that hormone functions may be involved in its regulation.

Localization and Characterization of the Interleukin 1 Immunoreactive Pool (IL-1 alpha and beta Forms) in Normal Human Epidermis

1989 ◽  
Vol 92 (6) ◽  
pp. 809-816 ◽  
Author(s):  
Liliane Didierjean ◽  
Denis Salomon ◽  
Yves Mérot ◽  
Georges Siegenthaler ◽  
Jean-Hilaire Saurat ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Human Epidermis ◽  
Normal Human
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