Gap Junction-Mediated Coupling in the Postnatal Anterior Subventricular Zone

2000 ◽  
Vol 22 (1-2) ◽  
pp. 34-43 ◽  
Author(s):  
João R.L. Menezes ◽  
Maira M. Fróes ◽  
Vivaldo Moura Neto ◽  
Roberto Lent
Keyword(s):  
Gap Junction ◽  
Subventricular Zone ◽  
Anterior Subventricular Zone

Spatiotemporal selectivity of response to Notch1 signals in mammalian forebrain precursors

Development ◽  
2001 ◽  
Vol 128 (5) ◽  
pp. 689-702 ◽  
Author(s):  
C.B. Chambers ◽  
Y. Peng ◽  
H. Nguyen ◽  
N. Gaiano ◽  
G. Fishell ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Cell Fate ◽  
Subventricular Zone ◽  
Cellular Proliferation ◽  
Precursor Cells ◽  
Temporal Cues ◽  
Short And Long Term ◽  
Cortical Regions ◽  
Anterior Subventricular Zone

The olfactory bulb, neocortex and archicortex arise from a common pool of progenitors in the dorsal telencephalon. We studied the consequences of supplying excess Notch1 signal in vivo on the cellular and regional destinies of telencephalic precursors using bicistronic replication defective retroviruses. After ventricular injections mid-neurogenesis (E14.5), activated Notch1 retrovirus markedly inhibited the generation of neurons from telencephalic precursors, delayed the emergence of cells from the subventricular zone (SVZ), and produced an augmentation of glial progeny in the neo- and archicortex. However, activated Notch1 had a distinct effect on the progenitors of the olfactory bulb, markedly reducing the numbers of cells of any type that migrated there. To elucidate the mechanism of the cell fate changes elicited by Notch1 signals in the cortical regions, short- and long-term cultures of E14.5 telencephalic progenitors were examined. These studies reveal that activated Notch1 elicits a cessation of proliferation that coincides with an inhibition of the generation of neurons. Later, during gliogenesis, activated Notch1 triggers a rapid cellular proliferation with a significant increase in the generation of cells expressing GFAP. To examine the generation of cells destined for the olfactory bulb, we used stereotaxic injections into the early postnatal anterior subventricular zone (SVZa). We observed that precursors of the olfactory bulb responded to Notch signals by remaining quiescent and failing to give rise to differentiated progeny of any type, unlike cortical precursor cells, which generated glia instead of neurons. These data show that forebrain precursors vary in their response to Notch signals according to spatial and temporal cues, and that Notch signals influence the composition of forebrain regions by modulating the rate of proliferation of neural precursor cells.

scholarly journals Diffusion MRI changes in the anterior subventricular zone following chemoradiation in glioblastoma with posterior ventricular involvement

2020 ◽  
Vol 147 (3) ◽  
pp. 643-652
Author(s):  
Nicholas Cho ◽  
Chencai Wang ◽  
Catalina Raymond ◽  
Tania Kaprealian ◽  
Matthew Ji ◽  
...  
Keyword(s):  
Subventricular Zone ◽  
Diffusion Mri ◽  
Mri Changes ◽  
Anterior Subventricular Zone

Initial stages of radial glia astrocytic transformation in the early postnatal anterior subventricular zone

2002 ◽  
Vol 52 (3) ◽  
pp. 251-265 ◽  
Author(s):  
José A. J. Alves ◽  
Patrick Barone ◽  
Simone Engelender ◽  
Maira M. Fróes ◽  
João R. L. Menezes
Keyword(s):  
Subventricular Zone ◽  
Radial Glia ◽  
Anterior Subventricular Zone

Potassium Currents in Precursor Cells Isolated From the Anterior Subventricular Zone of the Neonatal Rat Forebrain

1999 ◽  
Vol 81 (1) ◽  
pp. 95-102 ◽  
Author(s):  
R. R. Stewart ◽  
T. Zigova ◽  
M. B. Luskin
Keyword(s):  
Subventricular Zone ◽  
Action Potentials ◽  
Potassium Current ◽  
Potassium Currents ◽  
Precursor Cells ◽  
Neonatal Rat ◽  
Delayed Rectifier ◽  
Activation Time ◽  
Rat Forebrain ◽  
Anterior Subventricular Zone

Stewart, R. R., T. Zigova, and M. B. Luskin. Potassium currents in precursor cells isolated from the anterior subventricular zone of the neonatal rat forebrain. J. Neurophysiol. 81: 95–102, 1999. The progenitor cells from the anterior part of the neonatal subventricular zone, the SVZa, are unusual in that, although they undergo division, they have a neuronal phenotype. To characterize the electrophysiological properties of the SVZa precursor cells, recordings were made of potassium and sodium currents from SVZa cells that were removed from postnatal day 0–1 rats and cultured for 1 day. The properties of the delayed rectifier and A-type potassium currents were described by classical Hodgkin and Huxley analyses of activation and inactivation. In addition, cells were assessed under current clamp for their ability to generate action potentials. The A-type potassium current ( I K(A)) was completely inactivated at a holding potential of −50 mV. The remaining potassium current resembled the delayed rectifier current ( I K(DR)) in that it was blocked by tetraethylammonium (TEA; IC50 4.1 mM) and activated and inactivated slowly compared with I K(A). The conductance-voltage ( G- V) curve revealed that G increased continuously from 0.2 nS at −40 mV to a peak of 2.6 nS at +10 or +20 mV, and then decreased for voltages above +30 mV. Activation time constants were largest at −40 mV (∼11 ms) and smallest at 100 mV (∼1.5 ms). The properties of I K(A) were studied in the presence of 20 mM TEA, to block I K(DR), and from a holding potential of −15 mV, to inactivate both I K(DR) and I K(A). I K(A) was then allowed to recover from inactivation to negative potentials during 200- to 800-ms pulses. Recovery from inactivation was fastest at −130 mV (∼21 ms) and slowest at −90 mV (∼135 ms). Inactivation was voltage independent from −60 to +60 mV with a time constant of ∼15 ms. At steady state, I K(A) was half inactivated at −90 mV. G K(A) increased from 0.2 nS at −60 mV to a peak of 2.4 nS at +40 mV. Finally, the activation time constants ranged from ∼1.9 ms at −50 mV to 0.7 ms at +60 mV. The properties of I K(A) resembled those of I K(A) found in differentiating cerebellar granule neurons. Most SVZa cells had sodium currents (28/32 cells). However, in current clamp 11 of 12 cells were incapable of generating action potentials from voltages of −30 to −100 mV, suggesting that the available current densities were too low to support excitability.

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