scholarly journals Plasma Membrane Plasticity of Xenopus laevis Oocyte Imaged with Atomic Force Microscopy

2000 ◽  
Vol 10 (1-2) ◽  
pp. 99-107 ◽  
Author(s):  
Hermann Schillers ◽  
Timm Danker ◽  
Hans-Joachim Schnittler ◽  
Florian Lang ◽  
Hans Oberleithner
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
Xenopus Laevis ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

Observing Xenopus laevis oocyte plasma membrane by atomic force microscopy

Methods ◽  
2010 ◽  
Vol 51 (1) ◽  
pp. 106-113 ◽  
Author(s):  
Francesco Orsini ◽  
Massimo Santacroce ◽  
Paolo Arosio ◽  
Vellea Franca Sacchi
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
Xenopus Laevis ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Single-Molecule Atomic Force Microscopy Reveals Clustering of the Yeast Plasma-Membrane Sensor Wsc1

PLoS ONE ◽  
2010 ◽  
Vol 5 (6) ◽  
pp. e11104 ◽  
Author(s):  
Jürgen J. Heinisch ◽  
Vincent Dupres ◽  
Sabrina Wilk ◽  
Arne Jendretzki ◽  
Yves F. Dufrêne
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
Single Molecule ◽  
Membrane Sensor ◽  
Force Microscopy ◽  
Yeast Plasma Membrane ◽  
Atomic Force

Atomic force microscopy imaging ofXenopus laevis oocyte plasma membrane purified by ultracentrifugation

2008 ◽  
Vol 71 (6) ◽  
pp. 397-402 ◽  
Author(s):  
Massimo Santacroce ◽  
Francesco Orsini ◽  
Stefania Anna Mari ◽  
Massimo Marinone ◽  
Cristina Lenardi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
Microscopy Imaging ◽  
Force Microscopy ◽  
Atomic Force ◽  
Atomic Force Microscopy Imaging
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