Atomic force microscopy characterization of Xenopus laevis oocyte plasma membrane

2006 ◽  
Vol 69 (10) ◽  
pp. 826-834 ◽  
Author(s):  
F. Orsini ◽  
M. Santacroce ◽  
C. Perego ◽  
C. Lenardi ◽  
M. Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
Xenopus Laevis ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Characterization ◽  
Atomic Force Microscopy Characterization

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

Atomic force microscopy characterization of stable faces in cubic C60 and hexagonal C60, 2CCl4 single crystals

1994 ◽  
Vol 179 (3) ◽  
pp. 595-605 ◽  
Author(s):  
Bineta Keita ◽  
Louis Nadjo ◽  
René Céolin ◽  
Viatcheslav Agafonov ◽  
Daniel André ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Crystals ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Characterization ◽  
Atomic Force Microscopy Characterization

scholarly journals Atomic force microscopy characterization of collagen ‘nanostraws’ in human costal cartilage

Micron ◽  
2013 ◽  
Vol 44 ◽  
pp. 483-487 ◽  
Author(s):  
M. Stacey ◽  
D. Dutta ◽  
W. Cao ◽  
A. Asmar ◽  
H. Elsayed-Ali ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Costal Cartilage ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Characterization ◽  
Atomic Force Microscopy Characterization
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